Article
Chemistry, Analytical
Hiroyuki Terashima, Yui Mutoh, Sen-ichi Aizawa, Atsushi Taga, Ikko Mikami, Yutaka Itabashi, Kaname Tsutsumiuchi, Atsushi Yamamoto, Shuji Kodama
Summary: In this study, the enantiomers of abscisic acid were separated using HPLC with a phenyl column and a chiral mobile phase containing gamma-cyclodextrin. The optimum mobile phase conditions were determined to be 0.8% gamma-cyclodextrin, 4% acetonitrile, and 20 mM phosphate buffer at pH 6.0. It was observed that (R)-abscisic acid was eluted earlier than (S)-abscisic acid, suggesting a stronger interaction between (R)-abscisic acid and gamma-cyclodextrin. The proposed method successfully enantioseparated (S)-abscisic acid in a honey sample.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Engineering, Chemical
Huan Luo, Xiaoping Bai, Huixian Liu, Xin Qiu, Jianqiu Chen, Yibing Ji
Summary: Membrane-based chiral separation has been extensively studied, but traditional chiral membranes face a trade-off between permeability and selectivity. Recently, a new type of chiral covalent organic framework (CCOF) membranes have shown great promise. In this study, CCOF membranes were developed for the enantioseparation of chiral drugs. The results demonstrate that these membranes exhibit excellent chiral separation performance and stability, and show potential for industrial applications.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Chemistry, Physical
Maria Asghar, Ahmed Lakhani, Misbah Asif, Nadeem S. Sheikh, Muhammad Ali Hashmi, Ralf Ludwig, Hassan H. Hammud, Khurshid Ayub
Summary: In this study, CC2 cages were used to discriminate amino acids based on their chirality. The results showed that amino acids were physisorbed in the central cavity of the cage. Proline exhibited the strongest interaction with the cage and the highest chiral discrimination energy of 2.78 kcal/mol. The analysis also revealed that the S enantiomer had the maximum interactions in each case.
JOURNAL OF PHYSICAL CHEMISTRY A
(2023)
Article
Engineering, Chemical
Xin Qiu, Wenbei Chen, Yuting Chen, Jian Ke, Yibing Ji, Jianqiu Chen
Summary: In this study, three different chiral ionic liquids were synthesized and used to fabricate a chiral composite membrane for enantioseparation. The filtration conditions were optimized to improve the separation effect of the membrane. Molecular simulation was also conducted to study the separation mechanism and recognition mechanism of different chiral molecules. The prepared chiral membrane showed excellent enantioseparation performance, providing new insights for the design of chiral membranes.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Xuehua Wang, Jiaqi Wu, Xue Liu, Xin Qiu, Liqin Cao, Yibing Ji
Summary: Beta-cyclodextrin covalent organic frameworks (beta-CD COFs) with uniformly distributed chiral recognition sites and good chemical stability have shown great potential in enantioseparation. In this study, two novel functional beta-CD COFs were constructed through post-modification, and they exhibited excellent selectivity and repeatability in chiral separation. The mechanism of chiral recognition and enhanced enantioselectivity of functional beta-CD COFs were further demonstrated.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemical Research Methods
Meng Li, Xingjie Guo, Xin Di, Zhen Jiang
Summary: A novel beta-cyclodextrin derivative chiral stationary phase (EDACD) was synthesized and evaluated for enantioselective performance with antiinflammatory drugs and flavonoids. Factors affecting enantioseparation were investigated, showing promising results with adjustments in mobile phase composition and buffers. Molecular docking study indicated that hydrogen bonding and inclusion complexation played a crucial role in chiral recognition, suggesting a wider application potential for EDACD in chiral compound analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Luhuan Tang, Yanyan Chen, Mengru Wu, Tingting Tang, Yaqi Yao
Summary: In this study, the enantiomeric separation of furanocoumarins and dihydroflavones compounds was systematically investigated using four different polysaccharide-type chiral stationary phases. The effect of alcohol modifiers and alcohol content on enantiomeric separation was evaluated. Baseline separation was achieved for all eight compounds, with resolutions ranging from 1.52 to 23.11. Among the four chiral columns, Chiralpak IG exhibited the most universal and best enantioseparation ability. Steric hindrance, hydrogen bonding, and π-π interaction played major roles in chiral recognition on Chiralpak IG. This work provided a comprehensive analysis of the separation methods and reported new active chiral ingredients of traditional Chinese medicine, offering insights into the enantioseparation of furanocoumarins and dihydroflavones on chiral stationary phases.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Bao Tan Nguyen, Yoon Jung Choi, Kyeong Ho Kim, Gyu Yong Song, Hyung Min Kim, Jong Seong Kang
Summary: Plant-based bioactive substances are commonly used to treat inflammation due to their low toxicity and cost-effectiveness. This study developed a simple and effective method for chiral separation of decursinol and its derivatives, which have anti-cancer and anti-inflammatory properties. By using different polysaccharide-based chiral stationary phases (CSPs) and alcohol modifiers, the researchers achieved baseline separation and compared the chiral separation abilities of each column. Molecular docking simulations were conducted to understand the chiral recognition mechanism, and the results showed that hydrogen bonds, π-π interactions, and hydrophobic interactions played important roles. Overall, this study has practical implications for optimizing chiral separation techniques in the pharmaceutical and clinical industries.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Huiyun Qiu, Haiping Xiang, Mengyi Wen, Songlin Chen, Junchao Zhu, Shengqiang Tong
Summary: This study successfully used synthesized sulfobutyl ether-β-cyclodextrin (SBE-β-CD) as a chiral selector in countercurrent chromatography (CCC) to separate the enantiomers of Ketoconazole and Voriconazole. Two biphasic solvent systems were selected, and the influence factors of SBE-β-CD were investigated. Under optimized conditions, a high enantioseparation factor and peak resolution were achieved, and the purity of the separated stereoisomers reached 98.5%. Molecular docking was employed to investigate the formation of inclusion complexes. © 2023 Elsevier B.V. All rights reserved.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Engineering, Chemical
Xiong Liu, Shuhuan Chen, Yu Ma, Wenjie Xiao
Summary: The study demonstrates the considerable ability of the chiral ferrocenyl diphosphine ligand Mandyphos-Pd in separating 3-Chlorophenylglycine enantiomers, with significant influences of concentration and pH on enantioselectivity. The highest performance factor was achieved at pH 7.8 and Mandyphos-Pd concentration 1.2 mmol.L-1, with a possible recognition mechanism discussed based on experimental results.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Analytical
Weihao Bao, Chenning Zhang, Ming Yang, Ding Nan, Tingliang Liu, Xingjie Guo, Linlin Fang
Summary: In this study, an anionic carboxymethyl-beta-cyclodextrin (CM-beta-CD) silica hybrid monolithic column was prepared and optimized for chiral separations of ten racemic compounds, demonstrating satisfactory separation selectivity. Molecular modeling was used to elucidate the mechanism of chiral recognition. This novel chiral stationary phase shows promise for further analysis of chiral samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Lixia Chen, Xiangjun Li, Tingting Tian, Sen Yang, Huiqun Wang
Summary: In this study, a simple and efficient method for separating baclofen enantiomers by capillary electrophoresis was established. Molecular modeling and density functional theory were used to simulate and investigate the chiral resolution mechanism of electrophoresis, and the calculated intermolecular forces were directly presented by visualization software. Furthermore, the theoretical and experimental electronic circular dichroism (ECD) spectra of ionized baclofen were compared to determine the configuration of the dominant enantiomer in the nonracemic mixture.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Engineering, Chemical
Wenbei Chen, Huixian Liu, Yuting Chen, Jian Ke, Xin Qiu, Jianqiu Chen, Yibing Ji
Summary: Membrane separation technology has great potential for producing pure drugs, but there are challenges in terms of chiral membranes. To address these challenges, a chiral functional material was used to prepare a stainless steel cellulose acetate membrane. The optimized membrane showed improved enantioselectivity and solute permeation flux for racemic tryptophan, as well as successful enantiomeric separation of other racemates.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Engineering, Chemical
Xin Qiu, Jian Ke, Wenbei Chen, Huixian Liu, Xiaoping Bai, Yibing Ji, Jianqiu Chen
Summary: With the development of the pharmaceutical field, there is a growing demand for single enantiomer drugs. Chiral membrane separation technology has great potential in enantioseparation due to its simple preparation, continuous operation, and scalability.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Xinling Lu, Yuan Li, Jitong Yang, Yong Wang
Summary: This study proposes a concept of quasi-dual-chiral-channel enantioseparation platform, which can achieve wide spectrum chiral resolution and provides valuable insights for the rational design of versatile enantioseparation materials.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)