期刊
ANALYTICA CHIMICA ACTA
卷 731, 期 -, 页码 1-10出版社
ELSEVIER
DOI: 10.1016/j.aca.2012.04.010
关键词
Multidimensional liquid chromatography; Qualitative analysis; Quantitative analysis; Proteomics
资金
- National Basic Research Program of China [2012CB910604]
- National Nature Science Foundation [20935004, 21105099]
- Doctoral Fund of Dalian Institute of Chemical Physics [S201104]
- Creative Research Group Project by NSFC [21021004]
- Innovation method research of MOST [2010IM030500]
- Knowledge Innovation Project of Chinese Academy of Sciences
With the acceleration of proteome research, increasing attention has been paid to multidimensional liquid chromatography-mass spectrometry (MDLC-MS) clue to its high peak capacity and separation efficiency. Recently, many efforts have been put to improve MDLC-based strategies including top-down and bottom-up to enable highly sensitive qualitative and quantitative analysis of proteins, as well as accelerate the whole analytical procedure. Integrated platforms with combination of sample pretreatment, multidimensional separations and identification were also developed to achieve high throughput and sensitive detection of proteomes, facilitating highly accurate and reproducible quantification. This review summarized the recent advances of such techniques and their applications in qualitative and quantitative analysis of proteomes. (C) 2012 Elsevier B.V. All rights reserved.
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