Review
Chemistry, Multidisciplinary
Stanzin Lzaod, Tanmay Dutta
Summary: Antioxidants play an important role in food and their detection and quantification are crucial for protecting the body from oxidative damage. Biosensors, especially those using oxidoreductase enzymes, have emerged as a simple, fast, and economical method for the determination of antioxidants. This review discusses the different types of oxidoreductases utilized in biosensors for the detection of phenolic antioxidants in various food and beverage items. It also highlights alternative transduction systems and recent advances in enzyme immobilization and surface modification. Overall, it provides an overview of the latest developments in biosensor technology for phenolic antioxidant analysis in foodstuffs.
Article
Chemistry, Physical
Santiago Marzini Irranca, Sergio D. Garcia Schejtman, Anabella P. Rosso, Eduardo A. Coronado, Marisa Martinelli
Summary: In this study, a simple and convenient strategy for the synthesis of hybrid nanogels using chitosan and tannic acid with the incorporation of MNPs is described. The results show that these hybrid nanogels have the same peroxidase-like activity as bare MNPs, indicating their potential use in nanocatalytic medicine with improved biocompatibility and biodistribution.
Article
Spectroscopy
M. Muthu Meenakshi, Gowri Annasamy, Mugesh Sankaranarayanan
Summary: The study proposes a highly sensitive technique for the trace amount detection of pesticidal residues in centella powder. A graphene oxide gold (GO-Au) nanocomposite coated parafilm is developed as a substrate to facilitate dual surface enhanced Raman signal. The parafilm coated with GO-Au nanocomposites shows better Raman signal enhancement and can detect chlorpyrifos down to 0.1 ppm in centella herbal powder samples. This parafilm-based GO-Au SERS substrate can be used as a screening tool in quality control of herbal product manufacturing sectors.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Optics
Rawaa A. Faris, Sarah Kadhim Al-Hayali, Abdul Hadi Al-Janabi
Summary: This study demonstrated a multi-wavelength continuous-wave erbium-doped fiber laser using a highly nonlinear nanocomposite film, utilizing the four-wave mixing effect to increase the number of lasing wavelengths and intensity-dependent gain. The results showed good wavelength stability and improvement in output power, indicating the potential application of the prepared nanocomposite in multi-wavelength fiber lasers.
OPTICS COMMUNICATIONS
(2021)
Article
Environmental Sciences
Elham Pournamdari, Leila Niknam
Summary: A resonance Rayleigh scattering technique-based sensing method was developed for detecting Bentazone residual in water samples. The experiment results showed that the method had a very low detection limit and the chitosan-capped gold nanoparticles performed well as a sensor.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2023)
Article
Energy & Fuels
Adam Smolinski, Marek Stempin, Natalia Howaniec
Summary: In this paper, the content of chemical elements in waste was determined using ICP-OES and WDXRF techniques. To overcome the limitations of expensive and time-consuming analyses, a quick and inexpensive method for preliminary identification of the chemical composition of tested samples was developed. The samples were prepared as durable tablets for X-ray measurements. The limits of quantification were determined, and the uncertainty of the results was estimated by comparing them with a calibration method using standards and certified reference materials.
Article
Chemistry, Analytical
Vanessa Roman-Pizarro, Umair Gulzar, Juan Manuel Fernandez-Romero, Agustina Gomez-Hens
Article
Chemistry, Analytical
Angela Ecija-Arenas, Vanesa Roman-Pizarro, Juan Manuel Fernandez-Romero, Agustina Gomez-Hens
Article
Chemistry, Applied
Vanessa Roman-Pizarro, Juan Manuel Fernandez-Romero, Agustina Gomez-Hens
Article
Food Science & Technology
V. Roman-Pizarro, A. Gomez-Hens, J. M. Fernandez-Romero
FOOD ANALYTICAL METHODS
(2018)
Article
Agriculture, Multidisciplinary
Vanessa Roman-Pizarro, Juan Manuel Fernandez-Romero, Agusbna Gomez-Hens
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2014)
Article
Chemistry, Analytical
Marina Sierra-Rodero, Juan Manuel Fernandez-Romero, Agustina Gomez-Hens
Article
Chemistry, Analytical
Vanesa Roman-Pizarro, Manuel Ramirez-Gutierrez, Agustina Gomez-Hens, Juan Manuel Fernandez-Romero
Article
Chemistry, Analytical
Angela Ecija-Arenas, Vanesa Roman-Pizarro, Juan Manuel Fernandez-Romero
Article
Biochemical Research Methods
Angela Ecija-Arenas, Vanesa Roman-Pizarro, Juan Manuel Fernandez-Romero
Summary: Liposomes were separated and characterized using AF4 coupled with MALS, showing their potential for identifying liposomes based on the encapsulated material. Different liposome suspensions were prepared for analytical purposes, with each type of liposome having unique characteristics identified through various analytical methods.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Angela Ecija-Arenas, Vanesa Roman-Pizarro, Juan Manuel Fernandez-Romero
Summary: A method for monitoring the efficiency of separating hybrid magnetoliposomes using multiphase density gradient centrifugation coupled with a continuous flow system is described. Different h-MLs suspensions containing hydrophobic magnetic gold nanoparticles and various fluorophores were synthesized and separated using the MDGC system. The separation efficiency was tested and different fractions were identified for each separated h-MLs sample.
Article
Chemistry, Analytical
Angela Ecija-Arenas, Eva-Maria Kirchner, Thomas Hirsch, Juan Manuel Fernandez-Romero
Summary: A biosensor utilizing specific aptamers in a sensitive microzone for affinity reaction to detect kanamycin residues in agri-food samples has been developed. The sensor, based on SPR measurements and utilizing CVD graphene for immobilization of aptamers, showed excellent performance with a detection limit of 285 nmol L-1. The method demonstrated good recovery values and selectivity when applied to milk samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Agriculture, Multidisciplinary
Angela Ecija-Arenas, Vanesa Roman-Pizarro, Juan Manuel Fernandez-Romero
Summary: A new microfluidic approach using hybrid magnetoliposomes has been developed to determine aminoglycoside antibiotic residues in food, achieving high sensitivity and accuracy.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Vanesa Roman-Pizarro, Alba Maria Carrion-Escudero, Angela Ecija-Arenas, Juan Manuel Fernandez-Romero
Summary: A stopped-flow microfluidic system has been developed for monitoring glutathione peroxidase (GPx) activity and evaluating potential inhibitors. The system integrates a microfluidic chip, a magnetically retained enzyme microreactor (MRE mu R), and a remote luminescence detection using a focused bifurcated fiber optic bundle (BFOB) connected to a conventional spectrofluorometer detector. The method is based on the competitive oxidative chemical reactions between glutathione (GSH), homovanillic acid (HVA), and hydrogen peroxide in the presence of magnetically retained GPx-MNPs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Angela Ecija-Arenas, Antonio Zafra-Poyato, Juan Manuel Fernandez-Romero
Summary: A stopped-flow microfluidic fluorimetric biosensor has been developed to monitor alkaline phosphatase (ALP) activity and evaluate potential inhibitors. The biosensor integrates a magnetically retained enzyme microreactor (MRE mu R) in the reaction/detection zone of a microfluidic chip. The analytical signal is based on the fluorescence decrease observed in the dephosphorylation reaction of the substrate caused by sulfonamides. Evaluation of the method showed promising results with high recovery values.
Article
Chemistry, Analytical
Vanesa Roman-Pizarro, Angela Ecija-Arenas, Juan M. Fernandez-Romero
Summary: This study introduces a microfluidic-based biosensor for the determination of total cholesterol in serum. The biosensor integrates the reaction/detection zone of a microfluidic chip with a magnetically retained enzyme microreactor (MRE mu R) and uses remote fluorometric detection through a bifurcated fiber-optic bundle (BFOB) connected to a conventional spectrofluorometer. The method involves the enzymatic hydrolysis and oxidation of cholesterol at the microscale using immobilized enzymes on magnetic nanoparticles (MNPs). The biosensor demonstrates a wide dynamic range, high precision, and good correlation with other methods for cholesterol determination in serum.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)