Article
Chemistry, Analytical
Benjamin Charron, Alexandre Delorme, Caroline Dubois, Maryam Hojjat Jodaylami, Jean-Francois Masson
Summary: This study reveals the significant impact of human serum albumin (HSA) on the response and binding constants of sensing assays, while bovine serum albumin (BSA) does not show the same effect. The results also highlight the importance of the matrix protein/analyte/receptor interactions in determining the assay response.
Article
Fisheries
Nia Toshkova, Violeta Zhelyazkova, Sune Justesen, Jordan D. Dimitrov
Summary: Recently, studies have found that bats can harbor emerging pathogens without showing signs of infection, possibly due to their unique immune system. This study used surface plasmon resonance technology to investigate the interaction of IgG antibodies from three bat species with human and mouse FcRn. The results showed that bat IgG can bind to both human and mouse FcRn, with different affinities. This confirms the conservation of IgG-FcRn interaction and highlights the importance of the FcRn IgG salvaging system in bats.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Leila Khalili, Gholamreza Dehghan, Ali Akbar Moosavi-Movahedi, Yeojoon Yoon, Alireza Khataee
Summary: This study systematically characterized the binding of ASK and BSA to understand the pharmaceutics properties of the ligand and the conformational changes of BSA upon the addition of ASK. The results indicated a temperature-dependent binding constant between ASK and BSA. Thermodynamic analysis revealed viable hydrophobic and hydrogen bonds in the moderate-affinity complex formation. CD spectroscopy showed changes in the secondary structure of BSA, with an increased content of alpha-helices upon the addition of ASK. AFM and DLS techniques demonstrated slight conformational shrinkage and the formation of large particles upon ASK binding. Molecular modeling suggested allosteric modulation of BSA structure in the vicinity of the tryptophan residue. Overall, the results of ASK-BSA interaction have significant implications for expanding the bio-applications of shikonin.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Agriculture, Dairy & Animal Science
Kasey M. Schalich, Olivia M. Reiff, Blake T. Nguyen, Cassandra L. Lamb, Cecilia R. Mondoza, Vimal Selvaraj
Summary: Neonatal calf survival and health rely on sufficient IgG intake and passive immunity transfer. Colostrum accounts for only 25% of total IgG production, with early transition milk potentially contributing more IgG than colostrum.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Alexey M. Khalenkov, Malgorzata G. Norton, Dorothy E. Scott
Summary: We developed a method using Surface Plasmon Resonance (SPR) technology for screening and quantifying anti-influenza antibodies. The method showed a concentration-dependent inhibition of influenza hemagglutinin binding by specific antibodies in human plasma or intravenous immunoglobulin (IGIV) products. The SPR assay results correlated well with the conventional hemagglutination inhibition (HAI) assay results. This method can be used to identify high titer units in a high-throughput and time-saving manner.
Article
Chemistry, Analytical
Huiqing Yang, Qianqian Hou, Caifeng Ding
Summary: An antifouling sensing surface was constructed by crosslinking two-dimensional nanomaterial MXene with denatured bovine serum albumin (BSA), and a highly selective antifouling electrochemical biosensor was built. The biosensor, with a large specific surface area and good electrical conductivity, exhibited significantly enhanced sensitivity. Compared with BSA, the hydrogel formed through the peptide chain obtained from BSA provided a better antifouling sensing surface. The prepared biosensor quantitatively detects the concentration of IgG in complex human serum with high sensitivity.
Article
Biochemistry & Molecular Biology
Mojtaba Azari-Anpar, Kambiz Jahanbin, Pascal Degraeve, Farideh Tabatabaei Yazdi, Isabelle Adt, Nadia Oulahal, Didier Le Cerf
Summary: This paper describes the structural elucidation of Leuconostoc mesenteroides P35 exopolysaccharide (EPS-LM) produced by a goat cheese isolate. The analysis included determination of optical rotation degree, macromolecular characterization, sugar units and methylation analyses, FT-IR, 1D NMR spectroscopy, and 2D NMR spectroscopy. EPS-LM was found to be a high molecular weight dextran composed of D-glucose units with α(1 → 6) linkages and α(1 → 3) branches. The interaction between EPS-LM and bovine serum albumin was investigated using surface plasmon resonance, revealing the involvement of van der Waals and hydrogen binding forces. The findings suggest potential applications of Ln. mesenteroides P35α-D-glucan in the biopolymer, medical, and food industries.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Dairy & Animal Science
Anna Antonella Spina, Carlotta Ceniti, Francesca Trimboli, Domenico Britti, Vincenzo Lopreiato
Summary: This study evaluated MilkoScan FT-plus as a rapid tool for assessing colostrum quality by indirectly measuring immunoglobulin content through protein content measurement, focusing on bovine and ovine colostrum. The results showed good agreement between MilkoScan FT-plus and the reference method for immunoglobulin concentration measurement in colostrum.
Article
Chemistry, Analytical
Sung-Bae Kim, Genta Kamiya, Tadaomi Furuta, Nobuo Kitada, Shojiro A. Maki
Summary: Synthetic coelenterazine (CTZ) indicators were developed to quantitatively study human and bovine serum albumins (HSA and BSA) with high specificity. The CTZ indicators emit luminescence with specific modifications, and their colors and peak positions can determine the origins and mixing levels of albumins. The study also found that serum albumins catalyze the CTZ indicators and this catalytic reaction can be efficiently inhibited by specific inhibitors.
Article
Biochemistry & Molecular Biology
Ralf Weiskirchen, Sarah K. Schroeder, Sabine Weiskirchen, Eva Miriam Buhl, Bodo Melnik
Summary: Extracellular vesicles, such as exosomes, are small bilayered biovesicles released by cells, enriched with biologically active components. Milk exosomes play a key role in infant growth and development, while exosomes derived from different cell types have regenerative and immunomodulatory properties. Isolation protocols for milk exosomes allow the purification of highly enriched fractions, expressing typical exosomal protein markers.
Article
Chemistry, Physical
Dai Cheng, Tingting Zhao, Jiaping Zhou, Congying Yao, Yu Xi
Summary: This study investigates the impact of chlorogenic acid and its metabolites on the interaction between cadmium and bovine serum albumin through multispectral analysis, surface plasmon resonance, and docking studies.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Analytical
Pengfei Zhang, Rui Wang, Zijian Wan, Xinyu Zhou, Guangzhong Ma, Jayeeta Kolay, Jiapei Jiang, Shaopeng Wang
Summary: The article introduces a total internal reflection-based evanescent scattering microscopy technique that can detect single proteins without labeling and provides a high-precision measurement method for protein binding kinetics.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Soocheol Kim, Jin Hwa Ryu, Hoesung Yang, Kyuwon Han, Hyunseok Kim, Kwangsoo Cho, Soyoung Park, Sang Gi Hong, Kangbok Lee
Summary: This paper presents a spectrometer-based surface plasmon resonance imaging (SPRi) technique without mechanical scanning. A polarized broadband light source illuminates an object via a gold-coated prism, and the reflected light is spatially modulated by a digital mirror device (DMD) and measured with a spectrometer. Reflectance spectral images are reconstructed using the Hadamard transform (HT), and a refractive index (RI) map is visualized by analyzing the resonance peak shift at each image pixel. The method is demonstrated to have high resolution, sensitivity, and dynamic detection range, with experimental results validating the simulations.
Article
Chemistry, Multidisciplinary
Chien-Hsing Chen, Chang-Yue Chiang, Chin-Wei Wu, Chien-Tsung Wang, Lai-Kwan Chau
Summary: A novel fiber optic particle plasmon resonance biosensing platform for immunoglobulin G (IgG) detection was developed using a gold nanoparticle/graphene oxide composite as a signal amplification element. The sensor showed good analytical performance with a detection limit of 0.038 ng/mL and a linear relationship between sensor response and analyte concentrations. The sensitivity was attributed to the synergistic effect between graphene oxide and gold nanoparticles, with good reproducibility demonstrated for IgG detection.
Article
Immunology
Julie Van Coillie, Morten A. Schulz, Arthur E. H. Bentlage, Noortje de Haan, Zilu Ye, Dionne M. Geerdes, Wim J. E. van Esch, Lise Hafkenscheid, Rebecca L. Miller, Yoshiki Narimatsu, Sergey Y. Vakhrushev, Zhang Yang, Gestur Vidarsson, Henrik Clausen
Summary: Immunoglobulins G and their Fc gamma receptors play crucial roles in the immune system, and the N-glycan in IgG1 affects their interaction. This study genetically engineered N-glycan structures to investigate the role of N-glycosylation in IgG1:Fc gamma RIIIa binding. The findings demonstrate that afucosylated IgG1 binds with the highest affinity to oligomannose Fc gamma RIIIa.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Yisbel Eloisa Marin, Veronica Toccafondo, Philippe Velha, Simona Scarano, Stefano Tirelli, Alessandro Nottola, Yoonah Jeong, Hyunpyo Jeon, Sanghun Kim, Maria Minunni, Fabrizio Di Pasquale, Claudio J. Oton
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2019)
Article
Chemistry, Analytical
Annalisa Scroccarello, Flavio Della Pelle, Emiliano Fratini, Giovanni Ferraro, Simona Scarano, Pasquale Palladino, Dario Compagnone
Article
Chemistry, Analytical
Mariagrazia Lettieri, Pasquale Palladino, Simona Scarano, Maria Minunni
Summary: CuNCs exhibit unique optical properties, allowing sensitive detection of HSA. The method shows good sensitivity and reproducibility in serum and urine samples, offering a single protocol for both matrices.
Article
Chemistry, Analytical
Francesca Torrini, Lapo Renai, Simona Scarano, Massimo Del Bubba, Pasquale Palladino, Maria Minunni
Summary: This study examined the color development of common anthocyanins and anthocyanin-rich extracts using zinc-anthocyanin complexes as molecular probes. It was found that the absorbance in the blue region increased in the presence of excess zinc ions at acidic pH for certain derivatives, possibly due to base stabilization. The assay conditions were applied to natural extracts and demonstrated the effectiveness of this colorimetric method for quantifying anthocyanins.
Article
Chemistry, Analytical
Federica Battaglia, Francesca Bonelli, Micaela Sgorbini, Luigi Intorre, Maria Minunni, Simona Scarano, Valentina Meucci
Summary: In this study, a new sandwich-type immunoenzymatic assay based on a molecularly imprinted polymer (MIP) was developed for the determination of procalcitonin (PCT) in veterinary species. This method offers a promising analytical tool for the detection of disease biomarkers in veterinary patients, where validated assays are currently lacking.
ANALYTICAL METHODS
(2022)
Article
Biophysics
Francesca Torrini, Giada Goletta, Pasquale Palladino, Simona Scarano, Maria Minunni
Summary: Polycatecholamines (pCAs)-based molecularly imprinted polymers (MIPs) are a new generation of biocompatible ligand/receptor mimetics. Lysine (K) residues were found to effectively trigger the epitope imprinting process into a polynorepinephrine (PNE) matrix. Utilizing the N-3K tag strategy, excellent sensitivity (0.31 +/- 0.04 ng mL-1) and repeatability (avCV% = 4.5) were achieved in human serum samples. This strategy provides new insights for epitope design and tagging when native epitopes have low imprinting capabilities.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Francesca Torrini, Federica Battaglia, Pasquale Palladino, Simona Scarano, Maria Minunni
Summary: This study reports the development of a novel mimetic based on polynorepinephrine for IgG detection using label-free and real-time Surface Plasmon Resonance (SPR) sensing. The designed platform exhibited excellent analytical performances and stability for detecting IgG antibodies, making it a promising tool for serological point-of-care testing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Mariagrazia Lettieri, Michele Spinelli, Laura Caponi, Simona Scarano, Pasquale Palladino, Angela Amoresano, Maria Minunni
Summary: We developed a simple colorimetric assay for catecholamine detection in human urine, demonstrating the time-dependent formation and molecular weight of melanochrome (MC) and 5,6-indolequinone (IQ) derived from levodopa (LD), dopamine (DA), and norepinephrine (NE) oxidation. This assay can quantitatively detect LD and DA in urine, showing potential applicability in therapeutic drug monitoring and clinical chemistry.
Article
Chemistry, Analytical
Mariagrazia Lettieri, Simona Scarano, Laura Caponi, Andrea Bertolini, Alessandro Saba, Pasquale Palladino, Maria Minunni
Summary: We developed a simple and low-cost assay for copper in urine using a serotonin-derived fluorophore. The assay showed good reproducibility and low detection limits in buffer and artificial urine, and demonstrated excellent analytical performances in human urine samples. This is the first example of copper ion detection exploiting the fluorescence quenching of a biopolymer, offering a potential diagnostic tool for copper-dependent diseases.
Article
Chemistry, Analytical
Pasquale Palladino, Alberto Rainetti, Mariagrazia Lettieri, Giuseppe Pieraccini, Simona Scarano, Maria Minunni
Summary: This study reports a simple and inexpensive colorimetric method for quantifying carbidopa in anti-Parkinson drugs. The method is based on the condensation reaction between the hydrazine group from carbidopa and selected aldehydes in acidified hydroalcoholic solution. The results obtained by LC-MS analysis confirm the reliability of the method. The use of indole-3-carbaldehyde as a selective reagent provides better analytical performance compared to previous methods based on vanillin.
Article
Biotechnology & Applied Microbiology
Mariagrazia Lettieri, Pasquale Palladino, Simona Scarano, Maria Minunni
Summary: CuNCs offer advantages such as stability, ease of fabrication, affordability, unique photoluminescent properties, and high biocompatibility, making them a promising nanomaterial for various detection strategies. They demonstrate high sensitivity in different detection platforms, particularly in complex biological matrices.
SENSORS AND ACTUATORS REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Gina Elena Giacomazzo, Pasquale Palladino, Cristina Gellini, Gianluca Salerno, Veronica Baldoneschi, Alessandro Feis, Simona Scarano, Maria Minunni, Barbara Richichi
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)