Article
Chemistry, Applied
Xianlu Lei, Xinxin Xu, Li Wang, Liqiang Liu, Hua Kuang, Liguang Xu, Chuanlai Xu
Summary: In this study, a sensitive monoclonal antibody (mAb) 1B5 against eugenols was prepared and used for the development of a paper-based lateral-flow immunoassay (LFIA) using Eu-fluorescent microspheres sensor. The LFIA showed promising results for qualitative and quantitative detection of eugenols within 10 minutes. The recovery test and detection in fish samples demonstrated the reliability of the LFIA in real samples.
Article
Engineering, Environmental
Lin Luo, Shi-Qi Lin, Zhuo-Yu Wu, Hong Wang, Zi-Jian Chen, Hao Deng, Yu-Dong Shen, Wen-Feng Zhang, Hong-Tao Lei, Zhen-Lin Xu
Summary: A nanobody-alkaline phosphatase fusion protein-based fluorescent ELISA method was developed for highly sensitive detection of fenitrothion (FN) residue in food. The detection limit of this method was 0.14 ng mL-1, which is 25-fold lower than that of conventional colorimetric ELISAs.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Wenjie Jing, Shan Qiang, Zejun Jia, Qi Hao Shi, Xuan Meng, Mincong Yu, Hongyan Ma, Kai Zhao, Yujie Dai
Summary: Traditional methods for detecting organophosphorus pesticides are costly and complex, and also require expensive instruments. A nanozyme sensor array based on Ag2O nanospheres was developed to achieve simultaneous discrimination and efficient detection of a variety of OPs without the participation of natural enzymes. This work not only provides a convenient and high-efficiency method to identify OPs, but it also advances food safety and environmental monitoring.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Biotechnology & Applied Microbiology
Clare Mills, Katrina Campbell
Summary: There is an increasing demand for rapid and affordable in-field screening methods for low molecular weight (LMW) compound detection. This review examines the recent resurgence of using anti-idiotypes (Ab2s) and the potential role of phage display and nanobodies in improving LMW compound immunoassays.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Xianlu Lei, Lingling Guo, Liguang Xu, Hua Kuang, Chuanlai Xu, Liqiang Liu
Summary: In this study, an ultra-sensitive mAb 4D7 was prepared for the detection of fluoroquinolone residues in fish and milk. The developed immunochromatographic strip showed rapid and sensitive detection performance, with detection ranges of 0.027-5.5 μg kg(-1) and high recovery rates.
Article
Chemistry, Analytical
Chunhui Lu, Si Li, Wei Jiang, Qi Liu, Xiaomei Wang, Chen Yang, Quan Wang
Summary: A rapid and sensitive fluorescence-based immunochromatographic test was developed to quantitatively determine chloramphenicol levels in three food products. The method demonstrated high detection sensitivity and specificity, and could be completed for field detection within 30 minutes.
ANALYTICAL METHODS
(2022)
Article
Food Science & Technology
Lingyuan Xu, A. M. Abd El-Aty, Jae-Han Shim, Jong-Bang Eun, Xingmei Lei, Jing Zhao, Xiuyuan Zhang, Xueyan Cui, Yongxin She, Fen Jin, Lufei Zheng, Jing Wang, Maojun Jin, Bruce D. Hammock
Summary: This study developed a design and synthetic protocol for preparing highly sensitive and specific ATR monoclonal antibodies, which exhibited high specificity, sensitivity, and affinity.
Article
Spectroscopy
Yingfeng Qin, Gaojie Ye, Hao Liang, Ming Li, Jingjin Zhao
Summary: A novel amplified fluorescence polarization biosensor was developed for highly sensitive detection of organophosphorus pesticides using MnO2 nanosheets as the signal enhancer. The method efficiently suppresses AChE activity and decomposes MnO2 nanosheets to enhance FP signal, tremendously improving the sensitivity of detection for OPs such as diazinon.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Immunology
Lauren M. Walker, Andrea R. Shiakolas, Rohit Venkat, Zhaojing Ariel Liu, Steven Wall, Nagarajan Raju, Kelsey A. Pilewski, Ian Setliff, Amyn A. Murji, Rebecca Gillespie, Nigel A. Makoah, Masaru Kanekiyo, Mark Connors, Lynn Morris, Ivelin S. Georgiev
Summary: The development of novel technologies for discovering human monoclonal antibodies has been extremely valuable in combating infectious diseases. LIBRA-seq with epitope mapping is a next-generation sequencing technology that can determine residue-level epitopes for thousands of single B cells simultaneously, making it an efficient tool for high-throughput identification of antibodies against specific antigen epitopes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Yingjie Zhang, Changfei Duan, Qing Li, Yuchen Bai, Baolei Dong, Yingying Tang, Min He, Chenqi Hao, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: Polymyxin B and polymyxin E are last-line antibiotics used to treat infections caused by multidrug-resistant Gram-negative pathogens. A fluorescence polarization immunoassay method was developed for rapid screening of polymyxin B and polymyxin E in human serum, with high sensitivity and uniform specificity. The method showed efficient, accurate, and sensitive detection of polymyxins.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Yingjie Zhang, Changfei Duan, Qing Li, Yuchen Bai, Baolei Dong, Yingying Tang, Min He, Chenqi Hao, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: A fluorescence polarization immunoassay (FPIA) with high sensitivity and uniform specificity for rapid screening of polymyxin B (PMB) and polymyxin E (PME) in human serum was developed. The FPIA method demonstrated efficiency, accuracy, and sensitivity for the detection of polymyxins in human serum.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Shan Luo, Ruichen Peng, Ying Wang, Xianjun Liu, Jiali Ren, Wang Li, Ying Xiong, Sili Yi, Qian Wen
Summary: A series of near-infrared fluorescent probes were designed to monitor the presence of organophosphorus pesticides in food and live cells. The probes released a near-infrared fluorophore through catalytic hydrolysis of a carboxylic ester bond in the presence of carboxylesterase. Probe 1 exhibited excellent sensitivity and allowed for visualization of organophosphorus in biological systems. This study presents a promising strategy for tracking pesticide residues in food and biological systems.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Pimiao Zheng, Tao Peng, Jianyi Wang, Jing Zhang, Zile Wang, Yanfang Zhang, Zhenhui Ren, Sihan Wang, Haiyang Jiang
Summary: An ultrasensitive and rapid fluorescent immunoassay based on a broad-spectrum monoclonal antibody was developed to detect pyrrolizidine alkaloids in honey samples. By conjugating fluorescent gold nanoclusters with monoclonal antibodies, a qualitative and quantitative lateral flow immunoassay was established for the determination of four PAs in honey within 14 minutes.
Article
Chemistry, Analytical
Yuxiang Wu, Qi Fan, Yinuo Chen, Xia Sun, Guoqing Shi
Summary: The study successfully developed a sensitive monoclonal antibody for the simultaneous detection of carbofuran and its analogues, and compared the sensitivity of ELISA and LFIA methods for carbofuran detection. The results showed that ELISA method exhibited higher sensitivity. Additionally, both methods showed low cross-reactivity for other carbamate pesticides.
Article
Chemistry, Analytical
Shuang He, Demei Liang, Jincheng Xiong, Zile Wang, Pimiao Zheng, Huixia Zhang, Zhenhui Ren, Haiyang Jiang
Summary: In this study, a sensitive and rapid fluorescence polarization immunoassay (FPIA) method was developed for the screening of eight glucocorticoids (GCs) in beef samples. The method showed good correlation with liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method provides a time-saving and high-throughput tool for the simultaneous screening of GCs in beef, and contributes to food safety analysis.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)