Article
Biochemical Research Methods
Danting Liu, Shu Yang, Kanisha Kavdia, Jeffrey M. Sifford, Zhiping Wu, Boer Xie, Zhen Wang, Vishwajeeth R. Pagala, Hong Wang, Kaiwen Yu, Kaushik Kumar Dey, Anthony A. High, Geidy E. Serrano, Thomas G. Beach, Junmin Peng
Summary: A streamlined protocol has been developed to quantify over 9000 proteins with 0.5 μg of protein using 16-plex TMT coupled with LC/LC-MS/MS. Comparison with the standard protocol in 16 human brain tissue samples showed that the microgram-scale protocol had adequate sensitivity and reproducibility to detect differentially expressed proteins in disease-related pathways. This newly developed method holds promise for deep proteomic analysis at sub-microgram levels in biological and clinical samples.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Nutrition & Dietetics
Sarah Brajkovic, Nils Rugen, Carlos Agius, Nicola Berner, Stephan Eckert, Amirhossein Sakhteman, Claus Schwechheimer, Bernhard Kuster
Summary: Plants are crucial for sustainable global food supply, but the understanding of crop proteomes is limited. The presence of secondary metabolites in crop plants has hindered proteomic analyses, requiring individually optimized protein extraction protocols. In this study, a universal protein extraction protocol combined with an automated SP3 protocol and optimized micro-LC-MS/MS conditions was developed for high-quality proteomic analysis of crop plants. The workflow was successfully applied to analyze the proteomes of mature tomato fruits, demonstrating its robustness and potential for large-scale projects mapping crop tissue proteomes.
Article
Biochemical Research Methods
Dahang Yu, Zhe Wang, Kellye A. Cupp-Sutton, Yanting Guo, Qiang Kou, Kenneth Smith, Xiaowen Liu, Si Wu
Summary: A top-down TMT MS platform for confidently identifying and quantifying low molecular weight intact proteoforms in complex biological samples was developed by combining filter-SEC technique with high-performance size exclusion chromatography. This technique enables high-throughput analysis of intact proteoforms from Escherichia coli cell lysates, representing the first high-throughput TMT labeling-based, quantitative, top-down MS analysis suitable for complex biological samples.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Elsa Lundanes, Tuula A. Nyman, Frode S. Berven, Steven Ray Wilson
Summary: Single-cell analysis provides in-depth insights into diseases and diagnostics, with liquid chromatography playing a crucial role. The development of novel sample preparation techniques and improvements in mass spectrometry are enhancing the promise of single-cell analysis. However, technical challenges and the need for higher throughput and robustness may lead to the reinvention of alternative nano LC column formats.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Yoapoing Shi, Dipendu Mandal, Zhenbin Zhang, Yufen Zhao
Summary: We have developed a new protocol for determining D/L amino acids using a chiral phosphinate derivatization. (RP)-L-Menthyl phenylphosphinate successfully labeled eighteen pairs of amino acids except for Cys, and the chirality of amino acids was discriminated by 31P NMR. Seventeen pairs of amino acids were separated in 45 minutes using a C18 column, with resolution values ranging from 2.01 to 10.76. The lowest limit of detection was 10 pM in parallel reaction monitoring, highlighting the potential of chiral phosphine oxides in future chiral metabolomics.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Iva Michalus, Tran Van Nguyen, Jitka Viktorova, Pavel Cejnar, Jiri Santrucek, Stepanka Kuckova, Petra Sazelova, Vaclav Kasicka, Radovan Hynek
Summary: This study aimed to verify whether a method based on direct in-matrix protein digestion could be suitable for discriminating different pathological states of bone cell models cultivated in vitro. Using liquid chromatography-tandem mass spectrometry analysis of protein digestion products, the study successfully identified protein differences among different pathological states. Therefore, this method appears to be a promising tool for routine proteomic characterization of in vitro human bone models with induced different pathological states.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Miguel Angel Galvan-Morales
Summary: Proteomics in respiratory allergic diseases offers a wide range of techniques and programs for diagnosis, treatment, and immunotherapy. The main focus is on blocking cell diapedesis, modifying and blocking paratopes and epitopes, and inhibiting FceRI high-affinity receptors to prevent IgE binding. However, there is still a need for identifying allergens and cross-reactions through structural and epitope identification. The use of proteomics remains crucial for diagnostics and controlled immunotherapy systems. It is proposed to utilize this vision for treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Michaela Tesarova, Tereza Bouskova, Pavel Cejnar, Jiri Santrucek, Lenka Peterkova, Zdenek Fik, Petra Sazelova, Vaclav Kasicka, Radovan Hynek
Summary: Vestibular schwannoma is the most common benign neoplasm of the cerebellopontine angle. This study demonstrates the potential of in-sample protein digestion combined with LC-MS/MS analysis for fast and routine characterization of vestibular schwannoma at the molecular level.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Nutrition & Dietetics
Ilijana Begcevic Brkovic, Benedikt Zohrer, Markus Scholz, Madlen Reinicke, Julia Dittrich, Surab Kamalsada, Ronny Baber, Frank Beutner, Andrej Teren, Christoph Engel, Kerstin Wirkner, Holger Thiele, Markus Loeffler, Steffi G. Riedel-Heller, Uta Ceglarek
Summary: This study developed a mass spectrometry-based assay for identifying apoE phenotypes and profiling apolipoproteins. The assay showed good agreement with genotyping methods and revealed associations between apoE isoforms and plasma lipid levels in patients with cardiovascular disease. The study also confirmed differences in apoE concentration between subjects with mild cognitive impairment and controls.
Article
Chemistry, Analytical
Bert Vankeerberghen, Jeff Op de Beeck, Gert Desmet
Summary: Due to their dimensions, micropillar array columns are ideal for high-efficiency liquid chromatography separations in proteomics. The micropillar array concept has room to progress in terms of size reduction, which can lead to even higher-efficiency separations and applications. A comparison between first-generation (Gen 1) and second-generation (Gen 2) micropillar array columns on a chip showed that reducing the pillar and interpillar size did not affect the theoretical separation potential, resulting in significant reductions in analysis time.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Huan Sun, Suresh Poudel, David Vanderwall, Dong Geun Lee, Yuxin Li, Junmin Peng
Summary: Tandem mass tag (TMT) mass spectrometry is a popular labeling strategy for protein profiling. In this study, a 29-plex TMT method was developed and validated using pooled samples, demonstrating its ability to correct for noise and restore expected ratio values.
Article
Plant Sciences
Xiaojie Liu, Jonas Blomme, Kenny A. Bogaert, Sofie D'hondt, Thomas Wichard, Dieter Deforce, Filip Van Nieuwerburgh, Olivier De Clerck
Summary: This study analyzed the gametogenesis of the green seaweed Ulva mutabilis and identified a conserved RWP-RK transcription factor that plays a key role in sexual reproduction induction. Several potential master regulators of gametogenesis were also identified.
Article
Genetics & Heredity
Olivier Tytgat, Sonja Skevin, Dieter Deforce, Filip Van Nieuwerburgh
Summary: Nanopore sequencing for forensic purposes, combined with advanced basecallers and analysis methods, resulted in significantly improved STR genotyping accuracy compared to previous studies, while also achieving high accuracy in iso-allele detection.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2022)
Article
Spectroscopy
Peter Vandenabeele, Catarina Pereira Miguel, Anastasia Rousaki, Silvia Bottura Scardina, Mathilda Larsson-Coutinho, Miriam Pressato, Antonio Candeias
Summary: Raman spectroscopy is widely used in the analysis of art objects due to its non-destructive nature and the availability of mobile instruments. However, the stable positioning required by Raman spectroscopy limits its accessibility to every spot of large artifacts. Therefore, exploring the use of zoom lenses for Raman investigation at different working distances is valuable.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Multidisciplinary Sciences
Lien Provez, Bart Van Puyvelde, Laura Corveleyn, Nina Demeulemeester, Sigrid Verhelst, Beatrice Lintermans, Simon Daled, Juliette Roels, Lieven Clement, Lennart Martens, Dieter Deforce, Pieter Van Vlierberghe, Maarten Dhaenens
Summary: Omics studies are ideal for generating hypotheses on health and disease. While epigenomics focuses mainly on DNA methylation using sequencing technologies, the study of histone posttranslational modifications (hPTMs) using mass spectrometry is lagging behind. This article presents a comprehensive mass spectrometry-based preprocessed hPTM atlas, providing a valuable resource for exploring histone analysis and epidrug development.
Article
Chemistry, Analytical
Bart Van Puyvelde, Katleen Van Uytfanghe, Laurence Van Oudenhove, Ralf Gabriels, Tessa Van Royen, Arne Matthys, Morteza Razavi, Richard Yip, Terry Pearson, Nicolas Drouin, Jan Claereboudt, Dominic Foley, Robert Wardle, Kevin Wyndham, Thomas Hankemeier, Donald Jones, Xavier Saelens, Geert Martens, Christophe P. Stove, Dieter Deforce, Lennart Martens, Johannes P. C. Vissers, N. Leigh Anderson, Maarten Dhaenens
Summary: A new detection method called Cov2MS is developed using LC-MS and SISCAPA technology to enrich the nucleocapsid protein of SARS-CoV-2 in patient samples. This method shows high sensitivity and multiplexing capability, and can be used with various matrices. It is automatable and can process a large number of samples per day.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Magy Sallam, Mohamed Mysara, Mohammed Abderrafi Benotmane, Anne P. G. Crijns, Daan Spoor, Filip Van Nieuwerburgh, Dieter Deforce, Sarah Baatout, Pieter-Jan Guns, An Aerts, Raghda Ramadan
Summary: This study investigates DNA methylation in heart-irradiated rats and radiotherapy-treated breast cancer patients. It finds DNA methylation alterations in the blood of rats and breast cancer patients receiving radiation treatment. Additionally, certain genes' methylation levels show correlations with cardiovascular markers. This study provides clues for understanding the pathophysiology of RICVD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Steven Vermeulen, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. K. van der Veer, Dieter Deforce, Maarten Dhaenens, Jan de Boer
Summary: This study investigates how surface topography influences nuclear morphology, histone modifications, and gene expression. The authors find that micro-topographies result in a loss of histone acetylation and nucleoli abundance, as well as a reduction in gene expression associated with chromosome organization. They also show that micro-topographies can influence the proliferation and phenotype of mesenchymal stem cells, and this effect is reversible.
Article
Biochemistry & Molecular Biology
Annelies Raes, Eline Wydooghe, Krishna Chaitanya Pavani, Osvaldo Bogado Pascottini, Katleen Van Steendam, Maarten Dhaenens, Annekatrien Boel, Sonia Heras, Bjorn Heindryckx, Luc Peelman, Dieter Deforce, Filip Van Nieuwerburgh, Geert Opsomer, Ann Van Soom, Katrien Smits
Summary: While animal models have shown that culturing embryos in groups improves blastocyst yield and quality, the identity of the responsible embryotrophins remains largely unknown. This study identified cathepsin-L as an important embryotrophin in bovine in vitro embryo production, and demonstrated that supplementation of cathepsin-L significantly improved blastocyst development and quality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Willem Desmedt, Maarten Ameye, Osvaldo Filipe, Evelien De Waele, Filip Van Nieuwerburgh, Dieter Deforce, Lieven Van Meulebroek, Lynn Vanhaecke, Tina Kyndt, Monica Hofte, Kris Audenaert
Summary: This study showed that Z-3-hexenyl acetate-induced resistance (Z-3-HAC-IR) in rice can effectively protect against rice blast, sheath blight, and brown spot diseases, while also increasing rice yield.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Cell Biology
Benjamin Cappe, Mike Vadi, Eliza Sack, Ludivine Wacheul, Bruno Verstraeten, Sara Dufour, Julien Franck, Wei Xie, Francis Impens, An Hendrix, Denis L. J. Lafontaine, Peter Vandenabeele, Franck B. Riquet
Summary: Formation of extracellular vesicles (EVs) plays a crucial role in cell-to-cell communication, and there are differences in the biogenesis and composition of EVs during different types of cell death processes.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Art
Andrea Acevedo-Mejia, Mafalda Costa, Peter Vandenabeele, Luis Dias, Jose C. Frade, Milene Gil
Summary: This study analyzes the deteriorated green paint layers from mural paintings at the Maritime Station of Alcantara in Lisbon, Portugal. The analysis identifies the composition of the green pigments and the decay agents causing the visible degradation. The main cause of the deterioration is believed to be calcium sulphates, which are found to be widespread in all the analyzed samples.
INTERNATIONAL JOURNAL OF CONSERVATION SCIENCE
(2023)
Article
Food Science & Technology
Assia Saltykova, Julien Van Braekel, Nina Papazova, Marie-Alice Fraiture, Dieter Deforce, Kevin Vanneste, Sigrid C. J. De Keersmaecker, Nancy H. Roosens
Summary: The increasing number and diversity of GMOs require the development of advanced methods for their detection. This study presents a sequence-based database for annotated sequences of GM events, enabling the use of NGS technology for routine GMO detection and identification.
FOOD CHEMISTRY: MOLECULAR SCIENCES
(2022)
Correction
Mathematical & Computational Biology
Marie Deckers, Julien Van Braekel, Kevin Vanneste, Dieter Deforce, Marie-Alice Fraiture, Nancy H. C. Roosens
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2022)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
