期刊
ANALYTICA CHIMICA ACTA
卷 666, 期 1-2, 页码 97-101出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2010.03.060
关键词
Biosensors; Multiplexed immunoassay; Protein; Immunoglobulin; Gold nanoparticle; Amperometry
资金
- National Natural Science Foundation of China [20821063, 90713015, 20875044, 20845005]
- Department of Health of Jiangsu Province [RC2007069]
- Natural Science Foundation of Jiangsu [BK2008014]
This work proposed a simple, sensitive and low-cost multiplexed immunoassay by combining a disposable chip with gold nanoparticle (AuNP) as an electrochemical label. The immunosensors array as the disposable chip was firstly prepared by immobilizing capture antibodies on different screen printed carbon working electrodes by passive adsorption. With a sandwich mode, the analytes were then bound to the corresponding capture antibodies for further capture of the gold nanoparticle labeled antibodies. Gold nanoparticles were finally electrooxidized in 0.1 M HCl to produce AuCl4- for differential pulse voltammetric detection. Using human IgG and goat IgG as model targets, under optimal conditions this method achieved linear ranges from 5.0 to 500 and 5.0 to 400 mg L-1 with limits of detection of 1.1 and 1.6 ng mL(-1), respectively. This method eliminated completely electrochemical crosstalk between adjacent immunosensors due to the strong adsorption of the AuCl4- on the printed carbon surfaces. The immunosensors array showed acceptable precision, reproducibility and stability, and could be readily applied to multianalyte determination for clinical diagnostics. (C) 2010 Elsevier B.V. All rights reserved.
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