期刊
ANALYTICA CHIMICA ACTA
卷 673, 期 2, 页码 133-138出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2010.05.034
关键词
Electrochemical biosensor; Signal amplification; Enzyme; Au nanoparticles
资金
- National Natural Science Foundation of China [20875052]
- Natural Science Foundation of Shandong Province [JQ200805]
- Educational Administration of Shandong Province [J08LC09]
- Outstanding Adult-Young Scientific Research Encouraging Foundation of Shandong Province [2008BS05009]
Multiplex electrochemical detection of two DNA target sequences in one sample using enzyme-functionalized Au nanoparticles (AuNPs) as catalytic labels for was proposed. This DNA sensor was fabricated using a sandwich detection strategy, involving two kinds of capture probes DNA immobilized on glassy carbon electrode (GCE), and hybridization with target DNA sequences, which further hybridized with the reporter DNA loaded on the AuNPs. The AuNP contained two kinds of DNA sequences, one was complementary to the target DNA, while the other was noncomplementary to the target. The noncomplementary sequences were linked with horseradish peroxidase (HRP) and alkaline phosphatase (ALP), respectively. Enhanced detection sensitivity was obtained where the AuNPs carriers increased the amount of enzyme molecules per hybridization. Electrochemical signals were generated from the enzymatic products produced from the substrates catalyzed by HRP and ALP. Under optimal conditions, a 33-mer sequence could be quantified over the ranges from 1.5 x 10(-13) to 5.0 x 10(-12) M with a detection limit of 1.0 x 10(-13) M using HRP-AuNP as labels, and a 33-mer sequence could be quantified over the ranges from 4.5 x 10(-11) M to 1.0 x 10(-9) M with a detection limit of 1.2 x 10(-11) M using ALP-AuNP as labels. (c) 2010 Elsevier B.V. All rights reserved.
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