期刊
ANALYTICA CHIMICA ACTA
卷 634, 期 1, 页码 44-48出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2008.12.005
关键词
EcoRI endonuclease; Site-specific; DNA cleavage; Label-free; Electrochemical impedance; Cyclic voltammetry
资金
- National Natural Science Foundation of China [20705017]
Cyclic voltammetry (CV) combined with electrochemical impedance spectroscopy (EIS) were proposed to monitor the site-specific DNA cleavage by EcoRI endonuclease without using external label. The alteration of CV and EIS signal demonstrated that double-strands (dsDNA) contain recognition sequence was cleaved by EcoRI endonuclease. Real-time monitoring indicated that the dsDNA was cleaved by EcoRI more than 90% after 2 h of enzyme digestion time. Control experiment showed that the DNA cleavage by EcoRI endonuclease is site-specific for DNA sequence. Experimental results demonstrated that the efficiency of EcoRI cleavage was highly dependent on the concentration of EcoRI concentration in the range from 0.04 to 0.4 U mu L(-1) with one almost linear relationship. (C) 2008 Elsevier B.V. All rights reserved.
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