期刊
ANALYTICA CHIMICA ACTA
卷 652, 期 1-2, 页码 231-233出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2009.03.038
关键词
DNA extraction; Carrier RNA; Silica monoliths; Microfluidics
资金
- EPSRC [EP/D040930]
- Engineering and Physical Sciences Research Council [EP/D040930/1] Funding Source: researchfish
- EPSRC [EP/D040930/1] Funding Source: UKRI
DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25 ng) compared to the absence of RNA (5 ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications. (C) 2009 Elsevier B.V. All rights reserved.
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