Article
Chemistry, Analytical
Liya Zhou, Xitong Zhou, Congli Zhao, Yihang Liu, Yaoxuan Li, Li Ma, Ying He, Yanjun Jiang, Jing Gao
Summary: Mo2C/NCS, with excellent electron conduction channels and active sites, was used to fabricate AChE biosensors for OPs detection, showing wide linear ranges and outstanding detection limits.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Kai Niu, Yuying Zhang, Jiping Chen, Xianbo Lu
Summary: Covalent organic frameworks (COFs) are attractive materials for energy storage, catalysis, sorption, and sensing due to their porosity, stability, and tunable structure. However, their low conductivity limits their application in electrochemical sensing. In this study, an aza-fused x-conjugated COFs with carbonyl groups (COF1) was synthesized and used as electrode materials in electrochemical sensing for the first time. The COF1-based biosensor showed improved current response compared to the electrode without COF1, demonstrating the enhancing effect of carbonyl groups on signal detection.
Article
Biophysics
Kai Niu, Zicheng Zuo, Xianbo Lu, Lili Zou, Jiping Chen
Summary: In this study, a Cu@GDY nanocomposite was synthesized and used to fabricate an AChE biosensor for rapid detection of organophosphorus pesticides. The Cu@GDY nanocomposite exhibited excellent catalytic activity and the ability to regulate enzyme inhibition kinetics, resulting in amplified electrochemical signal and reduced oxidation potential for the pesticides. The biosensor achieved sensitive detection with high reproducibility and stability.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Shuling Xu, Min Li, Xia Li, Yanhui Jiang, Linlin Yu, Yue Zhao, Liyuan Wen, Qingwang Xue
Summary: In this study, a biosensor based on Ag/Au bimetallic nanoparticles was developed for simple and sensitive in situ detection of organophosphorus pesticide residues in food. The combination of AChE and CHO, along with the use of Ag/Au bimetallic nanoprobes as SERS signal probes, allowed for high sensitivity and stability. The method does not require complicated pretreatments and employs readily available reagents, showing great potential for in situ detection of organophosphorus pesticides in the field of food safety.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Chunming He, Ru Yan, Qingwang Xue, Shuxian Li, Huaisheng Wang
Summary: In this work, polyaniline (PANI) and overoxidized polyaniline (oPANI) nanorods doped by phytic acid (PA) were prepared as non-enzymatic malathion sensors. PA doping had a significant influence on the structural and morphological properties, conductivity, and detection performance of the electrode materials. PA-PANI exhibited high affinity towards malathion, hindering the redox reaction of PANI molecules, while PA-oPANI showed good catalytic performance, enhancing the electron transfer process. Both enzyme-free electrochemical sensors exhibited excellent stability, anti-interference ability, recovery in real samples, and wide linear ranges with low detection limits, indicating their potential as malathion sensors. Further exploration is needed for the reproducibility of the PA-oPANI sensor through anodic polarity processing.
JOURNAL OF MATERIALS SCIENCE
(2023)
Article
Chemistry, Analytical
Huzeyfe Celik, Saniye Soylemez
Summary: A novel conductive polymer biosensor based on Poly(BT) and AChE was developed for the determination of paraoxon. The biosensor showed good detection sensitivity and linear range, and was effectively used for pesticide detection in milk and tap water samples.
Review
Chemistry, Applied
Utkarsh Jain, Kirti Saxena, Vinita Hooda, Sapna Balayan, Amar Pal Singh, Mayukh Tikadar, Nidhi Chauhan
Summary: The use of organophosphates and carbamates pesticides poses a threat to human health and the environment, necessitating the monitoring of pesticide residues in food and soil for sensitive detection. Electrochemical biosensors offer a solution to overcome the limitations of conventional detection techniques, providing higher sensitivity, faster detection, reliability, and ease of use for pesticide detection.
Article
Electrochemistry
Changhua Chen, Jianping Zhou, Zhilei Li, Yan Xu, Tongtong Ran, Junhao Gen
Summary: This study presents a wearable biosensor for on-site monitoring of pesticide presence on crop surfaces. The biosensor shows excellent selectivity and recognition for methyl parathion and successfully detects it within a linear range of 1 ppb to 2 ppm. The combination of electrospinning and screen printing processes makes this sensor straightforward, sensitive, and efficient.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Zhenfeng Lin, Xin Liu, Yangzi Li, Changxiang Li, Liu Yang, Keke Ma, Zhenwei Zhang, Huayu Huang
Summary: Two-dimensional Mo2C/Mo2N composites were successfully synthesized and used for fabricating a biosensor with ultra-low detection limit for CPF. The biosensor showed satisfactory recoveries for CPF in real samples with acceptable relative standard deviations.
Article
Environmental Sciences
Danial Abdollahdokht, Gholamreza Asadikaram, Moslem Abolhassani, Hossein Pourghadamyari, Mojtaba Abbasi-Jorjandi, Sanaz Faramarz, Mohammad Hadi Nematollahi
Summary: The study evaluated the exposure of organophosphate pesticides (OPPs) and organochlorine pesticides (OCPs) in farmworkers' children aged 6 to 11 years in Jiroft city in southeastern Iran, finding harmful effects on the participants.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Spectroscopy
Yingfeng Qin, Gaojie Ye, Hao Liang, Ming Li, Jingjin Zhao
Summary: A novel amplified fluorescence polarization biosensor was developed for highly sensitive detection of organophosphorus pesticides using MnO2 nanosheets as the signal enhancer. The method efficiently suppresses AChE activity and decomposes MnO2 nanosheets to enhance FP signal, tremendously improving the sensitivity of detection for OPs such as diazinon.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Barbara B. Gerbelli, Pedro L. O. Filho, Bruna Cortez, Pedro T. Sodre, Mauricio D. Coutinho-Neto, Ian W. Hamley, Jani Seitsonen, Wendel A. Alves
Summary: The use of lipopeptide sequences as biomimetic microenvironments of acetylcholinesterase (AChE) has shown higher catalytic activities than natural enzymes in water solution, potentially valuable for pesticide detection.
NANOSCALE ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Rani, Faiz Ali, Mian Muhammad, Behisht Ara, Aftab Ali Shah
Summary: A simple and sensitive fluorescent sensor for chlortoluron detection was developed using fluorescent carbon dots synthesized via a hydrothermal protocol. The interaction between fructose carbon dots and Fe(iii) resulted in a fluorescent metastable state, which showed remarkable fluorescence quenching at 454 nm, further quenching occurred upon the addition of chlortoluron. The sensor exhibited a concentration range of 0.2-5.0 μg mL(-1) for chlortoluron detection, with a limit of detection of 0.0467 μg mL(-1), a limit of quantification of 0.14 μg mL(-1), and a relative standard deviation of 0.568%. The sensor also showed selective and specific recognition towards chlortoluron in real samples, with recoveries ranging from 95% to 104.3% in soil, water, and wheat samples.
Article
Multidisciplinary Sciences
Niloofar Mahmoudi, Fataneh Fatemi, Moones Rahmandoust, Fateme Mirzajani, Seyed Omid Ranaei Siadat
Summary: This study presents a fluorometric method using carbon quantum dots (CQDs) as a signal reporter for screening acetylcholinesterase (AChE) and its inhibitors. Different types of CQDs were synthesized and their fluorescence quenching properties were observed when exposed to various quenching ions. S-doped CQDs were found to be the most suitable fluorescent probe. The designed sensor showed low detection limits for malathion and chlorpyrifos.
Article
Chemistry, Applied
Ruijie Fu, Yiwen Wang, Yanlin Liu, Haoran Liu, Qiyang Zhao, Yaohai Zhang, Chengqiu Wang, Zhixia Li, Bining Jiao, Yue He
Summary: In this study, a sensitive fluorescent assay for detection of organophosphorus pesticides (OPs) based on activating the CRISPR-Cas12a system is proposed. By utilizing the catalytic properties of acetylcholinesterase (AChE) and DNAzyme, the fluorescence signal is amplified for OPs detection. The method shows low detection limits and holds great potential for application in agricultural products.
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)