Article
Biochemistry & Molecular Biology
Lauren M. Doolittle, Katherine Binzel, Katherine E. Nolan, Kelsey Craig, Lucia E. Rosas, Matthew C. Bernier, Lisa M. Joseph, Parker S. Woods, Michael Knopp, Ian C. Davis
Summary: This study found that inhibition of phosphatidylcholine synthesis affects the mitochondrial function of alveolar type II (ATII) epithelial cells in mice infected with influenza virus. Administration of the phosphatidylcholine precursor cytidine 5'-diphosphocholine (CDP-choline) improves this mitochondrial dysfunction.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Georgios Vasilopoulos, Roman Moser, Jonas Petersen, Meriyem Aktas, Franz Narberhaus
Summary: Bacterial membranes are primarily composed of phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and cardiolipin (CL). Some bacteria also produce phosphatidylcholine (PC), the hallmark of eukaryotic membranes. The biosynthesis pathways for PC and CL in bacteria have been studied, revealing enzyme activities involved in these processes.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Materials Science, Multidisciplinary
Fanyong Yan, Yueyan Zang, Jingxia Xu, Shan Guan, Li Chen, Jingru Sun
Summary: In this study, highly stable blue luminescent sulfur quantum dots (beta-SQDs) were synthesized rapidly using sublimated sulfur as the sulfur source and beta-cyclodextrin as the passivator. The incorporation of beta-cyclodextrin regulated the size and dispersion of beta-SQDs, enhancing their luminescence properties. The beta-SQDs showed satisfactory accuracy and recovery in the detection of real samples, indicating their potential role in environmental analysis.
MATERIALS RESEARCH BULLETIN
(2022)
Article
Biochemistry & Molecular Biology
Lini Huo, Xiaochen Liu, Yogini Jaiswal, Hao Xu, Rui Chen, Rumei Lu, Yaqin Nong, Leonard Williams, Yan Liang, Zhiruo Jia
Summary: We designed and synthesized a series of novel acridine-triazole and acridine-thiadiazole derivatives. These compounds showed high levels of antitumor activity against gastric cancer cells and bladder cancer cells, while exhibiting low toxicity to normal liver cells. Moreover, their pharmacological mechanisms and effects on cell cycle and apoptosis were evaluated. Furthermore, these compounds demonstrated low cytotoxicity to zebrafish but strong inhibition of the formation of zebrafish sub-intestinal veins, suggesting potential clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Karen G. Joao, Romeu A. Videira, Fatima Paiva-Martins, Patricia Valentao, David M. Pereira, Paula B. Andrade
Summary: Reversible acetylcholinesterase inhibitors were synthesized and screened, showing competitive inhibition of AChE in vitro. Among them, HO-C-16 emerged as the most potent inhibitor with high selectivity and affinity for AChE. These esters displayed no toxicity on neuronal cells.
Article
Chemistry, Applied
Olesia G. Kulyk, Olga S. Kolosova, Rostyslav P. Svoiakov, Dmytro Kobzev, Iryna Hovor, Ivanna M. Kraievska, Edward Sanin, Alexander Krivoshey, Zenoviy Yu Tkachuk, Anatoliy L. Tatarets
Summary: Novel homodimeric dyes based on the acridine orange chromophore show improved resistance and less inhibitory effect in qPCR compared to traditional nucleic acid labelling dyes. These dyes are suitable for high-resolution melt curve analysis and routine qPCR at a broad range of concentrations.
Article
Biophysics
Thais P. Pivetta, Quirina Ferreira, Tania Vieira, Jorge C. Silva, Sandra Simo, Paulo A. Ribeiro, Maria Raposo
Summary: Photodynamic therapy utilizes photosensitizer molecules for the treatment of diseases, but stability and encapsulation efficiency are influenced by lipid composition. Some lipid compositions can enhance stability of liposomes, while Acridine Orange has greater affinity for vesicles. Different liposomes have varied effects on cytotoxicity and phototoxicity in tumor cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Spectroscopy
Dina M. Beltukova, Victor P. Belik, Bogdan V. Semak, Irina V. Semenova, Andrey G. Smolin, Oleg S. Vasyutinskii
Summary: The experimental and theoretical studies of excited state dynamics in two alkyl derivatives of fluorescein, MitoFluo and C-8-Fl in solutions with liposomes were presented. The results showed that both types of liposomes significantly reduced the fluorescence quantum yield and caused a red shift of fluorescence maximum compared to pure fluorescein derivatives in solutions. The analysis of anisotropic fluorescence decay allowed for isolation of the contributions of fluorescein derivatives free in solution from those embedded in liposomes, suggesting that MitoFluo interacts more effectively with liposomes than C-8-Fl.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Hulya Bayir, John J. Maguire, Enrique Cadenas
Summary: Professor Valerian Kagan has made significant contributions in the field of redox biology, particularly in redox lipidomics and the identification of signaling pathways mediated by oxidatively modified phospholipids, which play essential roles in coordinating metabolism and physiology.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Chemistry, Analytical
Juan C. Stockert, Alfonso Blazquez-Castro
Summary: Many fluorophores, including acridine orange, exhibit orthochromatic vs. metachromatic behavior depending on their concentration and binding mode to cellular substrates. This article revisits the differential acridine orange fluorescence in selected biological examples, which can be used to identify single-stranded or double-stranded nucleic acids. The authors propose a potential molecular mechanism involving the degree of overlap of excited state orbitals.
Article
Biochemistry & Molecular Biology
Gemma Di Pompo, Katsuyuki Kusuzaki, Marco Ponzetti, Vito Ferdinando Leone, Nicola Baldini, Sofia Avnet
Summary: This study explores the use of acridine orange radiodynamic therapy (AO-RDT) as a targeted anticancer treatment for bone metastasis. The results demonstrate that AO-RDT is selectively toxic to carcinoma cells, effectively inhibits tumor expansion and tumor-induced bone resorption, without affecting osteoclasts.
Article
Polymer Science
Antonio D'Angelo, Luigi Vertuccio, Cristina Leonelli, Mohammad I. M. Alzeer, Michelina Catauro
Summary: The feasibility of encapsulating the potentially toxic acridine orange (AO) dye in a metakaolin based geopolymer while maintaining its structural integrity was investigated. Various analytical techniques were used to determine the structural, chemical, and mechanical stabilities of the final products, as well as the integrity and stability of the dye inside the geopolymer. The results showed that the addition of AO did not affect the thermal and mechanical properties of the geopolymer, and the dye remained stable within the matrix.
Review
Cell Biology
Alaumy Joshi, Travis H. Richard, Vishal M. Gohil
Summary: Studies on mitochondrial phospholipids have revealed their crucial role in mitochondrial bioenergetics and human health. The composition and function of these phospholipids have been highly conserved, and recent advances in understanding their transport mechanisms have further enhanced our understanding of mitochondrial phospholipid homeostasis. Additionally, disruptions in mitochondrial phospholipid biosynthesis have been linked to various genetic diseases, and promising therapeutic strategies are being developed for these disorders.
JOURNAL OF CELL SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
JunLi Yang, CanHua Li, YunHan Yang, Jie Gu, QiuLian Bao, JiaJia Lu, LiJiao Su, LiJuan Yang
Summary: In this study, three types of citric-acid-crosslinked beta-cyclodextrin adsorbents with different mass ratios were synthesized and investigated for their application in removing cationic dye Acridine Orange from water. The adsorbents were characterized and analyzed, and the effects of various factors on the adsorption performance were researched. The results showed that the adsorbents had high theoretical adsorption capacity and excellent recyclability, making them promising for environmentally friendly and industrial development.
Article
Spectroscopy
Lyudmila O. Kostjukova, Svetlana Leontieva, Victor V. Kostjukov
Summary: In this study, TD-DFT theory was used to obtain vibronic absorption spectra of AO dye in an aqueous solution, with the X3LYP/6-31G(d,p) level of theory showing the best agreement with experiment. Additionally, vibronic coupling and Boltzmann distribution were found to play a significant role in the absorption spectra of AO.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)