期刊
ANALYTICA CHIMICA ACTA
卷 622, 期 1-2, 页码 104-110出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2008.05.044
关键词
electrochemical DNA sensor; label-free detection; poly(indole-5-carboxylic acid); hybridization
An electrochemical method to directly detect DNA hybridization was developed on the basis of a new conductive polymer, which was, polymerized on the glassy carbon electrode with an indole monomer having a carboxyl group, indole-5-carboxylic acid (ICA). Hybridization with complementary, non- complementary and one-base mismatched DNA targets was studied by cyclic voltarnmetry (CV). Results showed a significant decrease in the current upon addition of complementary target. The change in peak current that was used as an index of sensor response was found to be linear with target concentration in the range of 3.34 x 10-9 to 1.06 x 10-1 M. The detection limit was 1.0 nM. (c) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据