Effects of interaction of folic acid with uranium (VI) and basic triphenylmethane dyes on resonance Rayleigh scattering spectra and their analytical applications

In pH 4.2-4.8 HAc-NaAc buffer solution, folic acid (FA) could react with uranium (VI) to form a 2:1 anionic chelate which further reacted with some basic triphenylmethane dyes (BTPMD) such as Ethyl Violet (EV), Methyl Violet (MV) and Crystal Violet (CV) to form 1:2 ion-association complexes. As a result, not only the absorption spectra were changed, but also the intensities of resonance Rayleigh scattering (RRS) were enhanced greatly and the new RRS spectra were observed. The maximum RRS wavelengths were located at 328 nm for EV system, 32S nm for MV system and 328 nm for CV system. The fading degree (Delta A) and RRS intensities (Delta I) of three systems were different. Under given conditions, the Delta A and Delta I were all directly proportional to the concentration of FA. The linear ranges and the detection limits of RRS methods were 0.0039-5.0 mu g mL(-1) and 1.2 ng mL(-1) for EV system, 0.0073-4.0 mu g mL(-1) and 2.2 ng mL(-1) for MV system, 0.014-3.5 mu g mL(-1) and 4.7 ng mL(-1) for CV system. The RRS methods exhibited higher sensitivity, so they are more suitable for the determination of trace FA. The optimum conditions, the influencing factors and the effects of coexisting substances on the reaction were investigated. The method can be applied to the determination of FA in serum and urine samples with satisfactory results. The structure of the ternary ion-association complex and the reaction mechanism were discussed in this work. (C) 2008 Elsevier B.V. All rights reserved.