Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection

A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2 M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively A linear response was observed at plasma levels ranged between 0.3 and 30 mu g mL(-1) for imipenem and 1.5 and 20 mu g mL(-1) for rifampicin. The detection limits were 0.07 mu g mL(-1) and 0.47 mu g mL(-1) for imipenem and rifampicin, respectively The method was applied to the determination of both compounds in mouse plasma samples. (c) 2007 Elsevier B.V. All rights reserved.