Article
Instruments & Instrumentation
Alina Syring, Zunhao Wang, Julia Molle, Hendrik Keese, Stefan Wundrack, Rainer Stosch, Tobias Voss
Summary: The three-dimensional morphology of polymeric microsphere layers reconstructed using confocal Raman microscopy revealed that refraction of the Raman laser beam at the curved surface of the spheres broadens the focus volume inside the spheres. The measured depth profiles are shifted and broadened, and the Raman signal of the underlying substrate is already observed above the microsphere layer. Ray-optical simulations successfully modeled the results.
APPLIED SPECTROSCOPY
(2022)
Article
Anatomy & Morphology
Heitor Hussni Casarin, Vicente Silva Mattos, Jarbas Caiado de Castro Neto, Michelle Alexandra Chinelatti
Summary: The study evaluated the chemical and morphological changes of femtosecond laser-irradiated enamel using Raman spectroscopy, CLSM, and SEM. Results showed a significant decrease in carbonate content and carbonate/phosphate ratio in enamel after laser irradiation, without altering its structure and morphology.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Review
Engineering, Multidisciplinary
Chang-Soo Kim, Hongki Yoo
Summary: Confocal microscopy utilizes a confocal aperture to eliminate out-of-focus blur, enabling high spatial resolution and contrast for precise three-dimensional reconstruction and measurement of microscopic samples. However, one limitation is its speed in 3D surface metrology applications.
MEASUREMENT SCIENCE AND TECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Alvaro Teijeira, Itziar Migueliz, Saray Garasa, Vaios Karanikas, Carlos Luri, Asunta Cirella, Irene Olivera, Marta Canamero, Maite Alvarez, Maria C. Ochoa, Ana Rouzaut, Maria E. Rodriguez-Ruiz, Miguel F. Sanmamed, Christian Klein, Pablo Umana, Mariano Ponz, Marina Bacac, Ignacio Melero
Summary: In this study, the performance of the CEA-CD3 T cell bispecific antibody cibisatamab in three-dimensional tumor organoids cocultured with T cells was investigated using time-lapse confocal microscopy. The results showed that the killing of tumor cells was dependent on the levels of surface CEA expression, and the higher affinity CEACAM5-CD3 bispecific antibody remained active on low CEA expressing organoids. Additionally, the coculture of tumor organoids, autologous fibroblasts, and T cells demonstrated a costimulatory effect of anti-FAP-4-1BBL antibody, leading to enhanced tumor cell killing.
Article
Plant Sciences
Mayya Razgonova, Yulia Zinchenko, Konstantin Pikula, Lyudmila Tekutyeva, Oksana Son, Alexander Zakharenko, Tatiana Kalenik, Kirill Golokhvast
Summary: This study analyzed the phytochemical substances in grains of Zea mays L. and found that anthocyanins are mainly located in the aleurone layer. A total of 56 compounds, including 30 polyphenols, were identified using tandem mass spectrometry. This method provides an efficient and rapid analysis of anthocyanins distribution in different plants, which is important for food plant breeding.
Article
Plant Sciences
Jan Knoblauch, Rainer Waadt, Asaph B. Cousins, Hans-Henning Kunz
Summary: Researchers have established three microscopy protocols to determine the in situ volume of chloroplasts using chlorophyll fluorescence, embedded CFP markers, and calculations from serial block-face scanning electron microscopy. The obtained data were validated by comparing them with mutant lines. The study also found that the size of guard cell plastids is not governed by the same division machinery as mesophyll plastids.
Article
Biotechnology & Applied Microbiology
Simin Masoudi, Mark Willcox
Summary: The aim of this study was to evaluate a new digestion method to quantify protein deposition on contact lenses. The results showed that immobilized trypsin can effectively digest protein deposits from the surface of contact lenses, and the ability to analyze surface protein concentrations may help in understanding the impact of surface deposition on clinical outcomes.
Review
Biochemical Research Methods
Tariq Sami Haddad, Peter Friedl, Navid Farahani, Darren Treanor, Inti Zlobec, Iris Nagtegaal
Summary: The authors discuss three-dimensional imaging pipelines for analyzing archival patient specimens, emphasizing the importance of utilizing these techniques. Advancements in new technologies have improved our ability to visualize tissues with subcellular resolution. Additionally, archival tissues are an overlooked resource that can provide valuable information with the appropriate methods.
Article
Optics
Chang-Soo Kim, Junyoung Kim, Hongki Yoo
Summary: The high-speed color 3D imaging method based on patterned illumination with a negative pinhole array overcomes the limitations of traditional pinhole arrays for brighter color images. Additionally, the proposed 3D image processing algorithm using 2D cross-correlation and multiple filtering masks significantly enhances the lateral resolution of color acquisition. This high-speed color 3D measurement technology with a negative pinhole array is demonstrated to be a useful tool for rapid and accurate non-contact measurements in various fields.
Review
Urology & Nephrology
Rui Santos, Max Burgi, Jose Maria Mateos, Alessandro Luciani, Johannes Loffing
Summary: The kidney is a complex organ responsible for maintaining water, ion, and solute balance. The nephron, composed of the renal corpuscle and tubular segments, is the functional unit of the kidney. The tissue architecture of the kidney plays a crucial role in its proper function, and kidney disease is often associated with structural abnormalities. Traditional microscopic analysis of 2-dimensional tissue sections lacks important 3-dimensional spatial information. Recent advancements in tissue preparation and imaging technologies, combined with machine-learning algorithms, offer new options for large-scale automated analysis of kidney structure and function.
KIDNEY INTERNATIONAL
(2022)
Article
Chemistry, Analytical
Carol Korzeniewski, M. Eric Peterson, P. Jay Kitt, D. Shelley Minteer, M. Joel Harris
Summary: Confocal Raman microscopy was used to quantify redox species near the electrode surface under potentiostatic control. Species diffusing into the confocal probe volume were detected, identified, and quantified based on their Raman scattering frequencies and intensities.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Tiziano Marzo, Giarita Ferraro, Lorena Maria Cucci, Alessandro Pratesi, Orjan Hansson, Cristina Satriano, Antonello Merlino, Diego La Mendola
Summary: The study shows that the anti-cancer drug oxaliplatin efficiently binds angiogenin, inhibiting its promoting effects on cell proliferation and migration. This suggests angiogenin as a potential target of oxaliplatin, indicating a new mechanism for the anti-neoplastic activity of this platinum drug and opening avenues for novel approaches in combined anti-cancer anti-angiogenic therapy.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaoping Liang, Shengxi Cao, Peisi Xie, Xudong Hu, Yusheng Lin, Jiehua Liang, Shengqi Zhang, Bai Xian, Hong Cao, Tiangang Luan, Zongwei Cai
Summary: A three-dimensional matrix-assisted laser desorption/ionization mass spectrometry imaging (3D MALDI-MSI) approach was developed for the first time to analyze whole-body zebrafish and identify altered lipids in a zebrafish model of Niemann-Pick disease type Cl (NPC1). The 3D fish model provided comprehensive information on the distribution of lipids in various organs, revealing significant alterations in sphingolipids and phospholipids in NPC1 mutant fish compared to wild type. This whole-body 3D MALDI-MSI approach unveiled unique lipid signatures for different NPC1-affected organs, offering insights into the link between impaired lipid storage and clinical symptoms.
ANALYTICAL CHEMISTRY
(2021)
Article
Computer Science, Artificial Intelligence
Steve Kench, Samuel J. Cooper
Summary: SliceGAN is a generative approach that constructs complex 3D images from 2D image examples, making it particularly useful for studying microstructured materials. It leverages high-quality 2D imaging techniques to create 3D datasets, eliminating the need for challenging 3D training data. This approach demonstrates promising potential for design optimization, with the ability to generate high-fidelity 3D datasets using a single representative 2D image.
NATURE MACHINE INTELLIGENCE
(2021)
Article
Chemistry, Multidisciplinary
Jingfan Wang, Xing Zhao, Da Li, Ya Wen, Weihao Wang, Bin Wang, Xiaoxuan Xu, Hua Bai, Weiwei Liu
Summary: This paper introduces a three-dimensional measurement system based on Autostereoscopy-Raman Spectrometry (ARS) for quality control of multilayer glass structure, manufacture and assembly of precision optical components, production process control of liquid crystal panel, and analyzing equipment for biomedical applications, with high accuracy in spatial localization and material identification within sub-micron levels.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemical Research Methods
Timothy P. Cleland, Elena R. Schroeter, Leonid Zamdborg, Wenxia Zheng, Ji Eun Lee, John C. Tran, Marshall Bern, Michael B. Duncan, Valerie S. Lebleu, Dorothy R. Ahlf, Paul M. Thomas, Raghu Kalluri, Neil L. Kelleher, Mary H. Schweitzer
JOURNAL OF PROTEOME RESEARCH
(2015)
Article
Chemistry, Analytical
Adaikkalam Vellaichamy, John C. Tran, Adam D. Catherman, Ji Eun Lee, John F. Kellie, Steve M. M. Sweet, Leonid Zamdborg, Paul M. Thomas, Dorothy R. Ahlf, Kenneth R. Durbin, Gary A. Valaskovic, Neil L. Kelleher
ANALYTICAL CHEMISTRY
(2010)
Article
Chemistry, Analytical
Jeremiah D. Tipton, John C. Tran, Adam D. Catherman, Dorothy R. Ahlf, Kenneth R. Durbin, Ji Eun Lee, John F. Kellie, Neil L. Kelleher, Christopher L. Hendrickson, Alan G. Marshall
ANALYTICAL CHEMISTRY
(2012)
Review
Biochemistry & Molecular Biology
Dorothy R. Ahlf, Paul M. Thomas, Neil L. Kelleher
CURRENT OPINION IN CHEMICAL BIOLOGY
(2013)
Review
Biochemistry & Molecular Biology
Jeremiah D. Tipton, John C. Tran, Adam D. Catherman, Dorothy R. Ahlf, Kenneth R. Durbin, Neil L. Kelleher
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemical Research Methods
Dorothy R. Ahlf, Philip D. Compton, John C. Tran, Bryan P. Early, Paul M. Thomas, Neil L. Kelleher
JOURNAL OF PROTEOME RESEARCH
(2012)
Article
Biochemical Research Methods
Ji Eun Lee, John F. Kellie, John C. Tran, Jeremiah D. Tipton, Adam D. Catherman, Haylee M. Thomas, Dorothy R. Ahlf, Kenneth R. Durbin, Adaikkalam Vellaichamy, Ioanna Ntai, Alan G. Marshall, Neil L. Kelleher
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2009)
Article
Biochemical Research Methods
Adam D. Catherman, Kenneth R. Durbin, Dorothy R. Ahlf, Bryan P. Early, Ryan T. Fellers, John C. Tran, Paul M. Thomas, Neil L. Kelleher
MOLECULAR & CELLULAR PROTEOMICS
(2013)
Article
Multidisciplinary Sciences
John C. Tran, Leonid Zamdborg, Dorothy R. Ahlf, Ji Eun Lee, Adam D. Catherman, Kenneth R. Durbin, Jeremiah D. Tipton, Adaikkalam Vellaichamy, John F. Kellie, Mingxi Li, Cong Wu, Steve M. M. Sweet, Bryan P. Early, Nertila Siuti, Richard D. LeDuc, Philip D. Compton, Paul M. Thomas, Neil L. Kelleher
Article
Biochemical Research Methods
Cong Wu, John C. Tran, Leonid Zamdborg, Kenneth R. Durbin, Mingxi Li, Dorothy R. Ahlf, Bryan P. Early, Paul M. Thomas, Jonathan V. Sweedler, Neil L. Kelleher
Article
Multidisciplinary Sciences
Dorothy R. Ahlf Wheatcraft, Xin Liu, Amanda B. Hummon
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2014)