期刊
ANALYST
卷 139, 期 1, 页码 299-308出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an01840g
关键词
-
资金
- National Natural Science Foundation of the People's Republic of China [31171700, 31101296]
- National High Technology Research and Development Program of China (National 863 Program of China) [2012AA101604]
- Natural Science Foundation of Shandong Province [ZR2010DQ025]
- Shandong Province Higher Educational Science and Technology Program [J10LB14]
An electrochemical aptasensor was developed for the detection of kanamycin based on the synergistic contributions of chitosan-gold nanoparticles (CS-AuNPs), graphene-gold nanoparticles (GR-AuNPs) and multi-walled carbon nanotubes-cobalt phthalocyanine (MWCNTs-CoPc) nanocomposites. The aptasensor was prepared by sequentially dripping CS-AuNPs, GR-AuNPs and MWCNTs-CoPc nanocomposites onto a gold electrode (GE) surface. During the above process, these nanomaterials showed a remarkable synergistic effect towards the aptasensor. CS-AuNPs, GR-AuNPs and MWCNTs-CoPc as the nanocomposites mediator improved electron relay during the entire electron transfer process and the aptasensor response speed. The electrochemical properties of the modified processes were characterized by cyclic voltammetry (CV). The morphologies of the nanocomposites were characterized by scanning electron microscopy (SEM). The experimental conditions such as the concentration of the aptamer, the time, temperature and the pH were optimized. Based on the synergistic contributions of CS-AuNPs, GR-AuNPs and MWCNTs-CoPc nanocomposites, the proposed aptasensor displayed high sensitivity, high specificity, a low detection limit (5.8 x 10(-9) M) (S/N = 3) and excellent stability. It was successfully applied to the detection of kanamycin in real milk spiked samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据