期刊
ANALYST
卷 138, 期 1, 页码 263-268出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an36398d
关键词
-
资金
- National Basic Research Program of China (973 Program) [2011CB707703]
- NSF of China [21075068]
- Tianjin Natural Science Foundation [11JCZDJC22200]
- Fundamental Research Funds for the Central Universities
- National Basic Research Program of China (973 Program) [2011CB707703]
- NSF of China [21075068]
- Tianjin Natural Science Foundation [11JCZDJC22200]
- Fundamental Research Funds for the Central Universities
An electrochemiluminescent (ECL) lead biosensor was developed based on GR-5 lead-dependent DNAzyme for lead recognition and intercalated ruthenium tris(1,10-phenanthroline) (Ru(phen)(3)(2+)) as the ECL probe. The thiol-modified substrate was first immobilized on the surface of the gold electrode via gold-sulfur self-assembly. Subsequently, the hybridization of DNAzyme and its substrate and the automatic intercalation of Ru(phen)(3)(2+) proceeded. Intercalated Ru(phen)(3)(2+) can transfer electrons through double-stranded DNA to the electrode and its electrochemiluminescence was excited with a potential step using tripropylamine as the coreactant. In the presence of lead, the substrate cleaves at the scissile ribo-adenine into two fragments. The dissociation of DNAzyme occurs, leading to the releasing of intercalated Ru(phen)(3)(2+) accompanied by a decrease in the intensity of electrochemiluminescence. A quantity of lead can be calculated from this decrease. The biosensor is highly sensitive and specific, along with an ultra-low limit of detection of 0.9 pM and a dynamic range from 2 to 1000 pM. It enables analysis of trace amounts of lead in serum samples. The combination of the intercalated-Ru(phen)(3)(2+) ECL probe and the cofactor-dependent DNAzyme may push the performance of cofactor-sensing tactics to the extreme.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据