Article
Biochemical Research Methods
Ruben R. G. Soares, Ahmad S. Akhtar, Ines F. Pinto, Noa Lapins, Donal Barrett, Gustaf Sandh, Xiushan Yin, Vicent Pelechano, Aman Russom
Summary: This study aimed to develop a cost-effective viral load detection system for point-of-care COVID-19 diagnostics. By developing an integrated modular centrifugal microfluidic platform, rapid amplification and detection of viral RNA from nasopharyngeal swab samples with high specificity and sensitivity were achieved.
Article
Food Science & Technology
Silvia Petronilho, Andre Oliveira, M. Rosario Domingues, Fernando M. Nunes, Manuel A. Coimbra, Idalina Goncalves
Summary: The addition of spent frying oil (SFO) and KOH during starch-based film production can greatly improve the transparency, hydrophobicity, and flexibility of the films, making them more suitable for various applications. Experimental results using a one-step approach have shown the effectiveness of this method in enhancing material properties.
Article
Green & Sustainable Science & Technology
Josefina Sullivan-Porras, Maria Badilla-Sanchez, Renato Rimolo-Donadio, Federico Masis-Melendez
Summary: This study investigates the application of hydrophobic-coated sand as a hydrophobic capillary barrier. Synthetic sand was coated with fatty acids extracted from palm oil sludge, and various methods were used to evaluate its contact angle, water holding capacity, hydraulic conductivity, and effectiveness as a capillary barrier.
Article
Chemistry, Analytical
Siyuan Wang, Hong Duan, Jianhan Lin
Summary: This study developed a bacterial detection platform based on chitosan electrostatic adsorption for nucleic acid purification, RAA-T7-CRISPR/Cas13a system for nucleic acid quantification, and a centrifugal microfluidic platform for full-automatic operation. The platform showed the capability of automatically detecting Salmonella within 1 h, with a detection limit of 10 CFU/mL. The average recovery and standard deviation of the platform were 106.9% and 6.4%, respectively.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Rooge Suvanasuthi, Sarin Chimnaronk, Chamras Promptmas
Summary: This study demonstrates a simple technique for creating hydrophobic barriers on paper for muPADs using a commercialized 3D printer. The designed prototype is capable of discriminating dengue virus serotypes based on small nucleotide sequence differences, providing a versatile platform for rapid prototyping of POCT.
Article
Engineering, Biomedical
Zhilin Dai, Chengpan Li, Zhengdi Shi, Shibo Li, Tianzhi Luo, Weiping Ding
Summary: This study proposed a hollow fiber-based biomimetic glomerulus chip that can regulate filtration in response to blood pressure and hormone levels. The cell morphology, viability, and metabolic function were evaluated and compared under fluidic and static conditions. In addition, the diffusion of fluorescein isothiocyanate (FITC)-labeled substances was monitored, and for the first time, the chip achieved on-chip filtration regulation in response to the hormone atrial natriuretic peptide. The potential application of the chip in drug nephrotoxicity evaluation was also demonstrated.
Article
Materials Science, Biomaterials
Dongxu Lan, Yulian Shang, Hongxian Su, Minhua Liang, Yang Liu, Haofei Li, Qi Feng, Xiaodong Cao, Hua Dong
Summary: This study presents a novel method to fabricate biomimetic hollow hydrogel microfibers using 3D printing-assisted soft lithography, showing excellent mass transport capacity, biocompatibility, and mechanical strength. The hollow hydrogel microfibers exhibit promising potential as candidates for blood capillaries.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Article
Biochemical Research Methods
Hong Hanh Tran, Kieu The Loan Trinh, Nae Yoon Lee
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2013)
Article
Chemistry, Analytical
Hong Hanh Tran, Wenming Wu, Nae Yoon Lee
SENSORS AND ACTUATORS B-CHEMICAL
(2013)
Article
Biochemical Research Methods
Hong Hanh Tran, Bin Chen, Hui Chen, Rima Menassa, Xiuming Hao, Mark Bernards, Norman P. A. Huner, Aiming Wang
JOURNAL OF VIROLOGICAL METHODS
(2019)
Article
Virology
Sizolwenkosi Mlotshwa, Nitika Khatri, Jane Todd, Hong Hanh Tran, Lucy R. Stewart
Summary: In this study, a full-length infectious clone of an East African isolate of maize yellow mosaic virus (MaYMV) was constructed and shown to be infectious in maize and Nicotiana benthamiana plants. Symptomatic infections were observed in maize plants while infections in N. benthamiana were asymptomatic. The cloned virus was also shown to be aphid transmissible to maize with experimental transmission rates up to 97%, indicating a breakthrough for studies on molecular interactions between maize, polerovirus, and aphids.
Article
Virology
Sizolwenkosi Mlotshwa, Nitika Khatri, Kristen Willie, Junhuan Xu, Jane Todd, Hong Hanh Tran, Lucy R. Stewart
Summary: In this study, the expression strategy of two coat proteins of maize rayado fino virus (MRFV) was investigated, and their roles in virus transmission were examined. The results showed that CP1 is essential for leafhopper transmission.
Article
Plant Sciences
Surapathrudu Kanakala, Cesar A. D. Xavier, Kathleen M. Martin, Hong Hanh Tran, Margaret G. Redinbaugh, Anna E. Whitfield
Summary: This study reports the construction of an infectious clone for the maize-infecting Alphanucleorhabdovirus maydis, which is the first efficient NSR vector for maize. The infectious clone was established using reverse genetics technologies and a GFP reporter gene was inserted to monitor virus infection. The results demonstrate the stability and efficiency of this infectious clone in plant and insect hosts.
MOLECULAR PLANT PATHOLOGY
(2023)