Article
Spectroscopy
Ying Bao, Kexin Han, Zhifang Ding, Yue Li, Ting Li, Ming Guan, Guixin Li
Summary: Polypyrrole-luminol-AuNPs nanocomposites were prepared and utilized for a label-free electrochemiluminescence (ECL) immunosensor for detecting carbohydrate antigen 153 (CA153). The nanocomposites exhibited excellent ECL activity and good film-forming property, allowing them to be fixed on electrode surface without the need for additional film-forming materials.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Yixin Nie, Peilin Wang, Qiang Ma, Xingguang Su
Summary: Based on electronic metal-support interaction (EMSI), a gold single atom confined MXene (AuSA/MXene) heterostructure was developed as a highly efficient electrochemiluminescence (ECL) functional material, which improved the electrochemical properties and expanded the sensing application of MXenes. The AuSA/MXene heterostructure exhibited high catalytic activity and electrical activity, enabling the construction of a high-efficiency ECL sensor for specific substance detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Mengxia Yan, Sinuo Feng, Linying Yu, Yu Xue, Jianshe Huang, Xiurong Yang
Summary: In this study, the aggregation-induced electrochemiluminescence (AIECL) of a distyrylarylene derivative, 4,4'-bis(2,2-diphenylvinyl)-1,1'-biphenyl (DPVBi), was investigated for the first time, showing significantly enhanced luminescence efficiency in organic/water mixtures. DPVBi nanobulks (DPVBi NBs) were prepared and used to develop a label-free immunosensor for cardiac troponin I (cTnI) in aqueous media, with high sensitivity and specificity.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Eduardo Zapp, Daniela Brondani, Tania Regina Silva, Edivandro Girotto, Hugo Gallardo, Iolanda Cruz Vieira
Summary: According to the World Health Organization, cardiovascular diseases (CVDs) are the leading cause of mortality and morbidity worldwide. Therefore, the development of electrochemical biosensors for detecting CVD markers has become an important diagnostic strategy. In this study, a glassy carbon electrode was modified with liquid crystal and gold nanoparticles to successfully detect cardiac troponin I, showing good diagnostic capacity for point-of-care applications.
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mingquan Guo, Jiangnan Shu, Dexin Du, Mohammad A. Haghighatbin, Di Yang, Zhiping Bian, Hua Cui
Summary: A novel ECL nanoluminophore was synthesized in this work for detecting cardiac troponin I with a wide linear dynamic range and low detection limit. The sensor can accurately determine the target analyte based on the ratio of three ECL peaks and offers self-correction analysis capabilities.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Food Science & Technology
Zhiping Yu, Chengqiang Li, Jiashuai Sun, Xia Sun, Guodong Hu, Thierry Noguer
Summary: The accumulation of pesticide residues poses a significant threat to human health and ecological systems. In this study, an electrochemiluminescence aptasensor was developed for the rapid detection of malathion residues. The sensor showed excellent specificity, repeatability, and stability, and was successfully applied to detect pesticide residues in authentic samples.
Article
Chemistry, Analytical
Lingya Ning, Yumin Bai, Zixiao Wang, Wenjun Wen, Junping Wang
Summary: A novel and rapid electro-chemiluminescence (ECL) immunosensor was successfully fabricated for ultrasensitive detection of 3-NT. The sensor utilized gold nanoparticles as the luminophore and electropolymerized polyaniline to promote the luminescence of the nanoparticles. It exhibited good analytical performance for 3-NT detection with a wide linear detection range and low detection limit, and had high recovery rate in practical sample applications.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Yu Du, Siqi Yu, Huangxian Ju
Summary: A quenching-typed electrochemiluminescence immunosensing method was proposed for protein detection using tungsten-based polyoxometalate nanoclusters and free radicals. The method demonstrated excellent detection limit and detectable range.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Wuyi Zhang, Lanlan Chen, Kui Yang, Lijuan Wang, Baoquan Han, Shiguo Sun, Jia Wen
Summary: A sensitive electrochemiluminescence (ECL) immunosensor was developed using functionalized metal organic layers (MOLs) for the detection of carcinoembryonic antigen (CEA). The immunosensor utilized gold nanoparticles (AuNPs) grown on ultrathin two-dimensional MOLs, which acted as electron transfer accelerators and labels for antibody conjugation. The AuNPs@Ir-Zr-MOL nanocomposite exhibited high ECL efficiency and could detect CEA concentration in the range of 1.00 pg mL-1 to 100 ng mL-1 with a detection limit of 0.200 pg mL-1 (S/N = 3).
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Engineering, Environmental
Zilian Zhang, Ding Jiang, Hanling Ding, Xueling Shan, Wenchang Wang, Hiroshi Shiigi, Zhidong Chen
Summary: This study presents a novel luminol-DO electrochemiluminescence system with increased dissolved oxygen content and accelerated reduction efficiency by using micro-nano bubbles and CoS2/S-Ti3C2 heterostructure. It demonstrates excellent performance for the detection of chloramphenicol and provides a new avenue for the development of high-performance luminol-DO ECL systems.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Saloni Kakkar, Sakshi Chauhan, Manojkumar Rohit, Vijayender Bhalla
Summary: This study reports a label-free ultrasensitive electrochemical aptasensor (GO@GNP-A) for detecting cardiac biomarker Troponin I (TnI). The nanohybrid of graphene oxide (GO) and gold nanoparticles (GNP-A) is self-assembled to form the nanohybrid (GO@GNP-A), onto which the biomarker protein is analyzed. TnI interactions at the aptamer biointerfaced nanohybrid surface cause electrochemical signal enhancement. The sensitivity of this nanohybrid aptasensor platform is 0.001 pg/mL. The study has been further validated in clinical samples for early, sensitive, and efficient point-of-care detection of TnI in cases of Acute Myocardial Infarction (AMI).
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Aihua Lyu, Yisha Wang, Hua Cui
Summary: In this study, a new mechanism for enhancing chemiluminescence (CL) was developed through the coembedding of N-(aminobutyl)-N-(ethylisoluminol) (ABEI) and Co2+ into gold nanoparticle-modified covalent-organic frameworks (COFs). The confinement of ABEI and Co2+ into Au-COF brought over 20-fold enhancement of CL intensity. This work proposes a novel enhancement mechanism for CL based on crystalline porous materials, which provides a new perspective for the development of CL-functionalized materials for biosensors and bioassays.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Shuting Cheng, Rui Xu, Fengzhen Yang, Jingcheng Huang, Xia Sun, Xue Huang, He Li, Falan Li, Yemin Guo, Mohammad Hasanzadeh, Yelong Zhu
Summary: A novel sandwich electrochemiluminescence aptasensor was developed for highly sensitive detection of kanamycin. The aptasensor used PAMAM, molybdenum disulfide, and multi-walled carbon nanotubes as the substrate to improve the ECL intensity. The sandwiched aptasensor formed between aptamer1 and aptamer2 resulted in a proportional increase of ECL intensity. The aptasensor exhibited a wide linear range and low detection limit.
BIOELECTROCHEMISTRY
(2022)
Article
Acoustics
Lin Du, Huixin Zhang, Zhenyu Wang, Tingting Zhuang, Zonghua Wang
Summary: A strategy combining ultrasound and nanomaterial was designed to enhance the ECL signal of the luminol-O2 system. Ultrasound pretreatment generated ROS in situ and triggered the ECL signal, while 1T/2H MoS2 catalyzed the oxidation of luminol and further enhanced the ECL response. The ECL biosensor, combined with catalytic DNA assembly, showed excellent performance for the detection of miRNA-155 with a detection limit of 0.057 fM.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Jue Wang, Chao Hu, Yi-sha Wang, Hua Cui
Summary: In this study, a chemiluminescent 2D metal-organic framework (MOF) with dual functionality of a chemiluminescence reagent and noble metal nanoparticles was developed. The material exhibited strong and stable chemiluminescence properties and outstanding peroxidase-mimicking activity, making it suitable for the design of highly sensitive biosensors.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Fang Li, Anqi Zhao, Zimu Li, Yachao Xi, Jianming Jiang, Jianbo He, Jue Wang, Hua Cui
Summary: In this study, multifunctional hydrogel beads with intense and multicolor chemiluminescence (CL) were successfully prepared and applied for label-free CL imaging immunoassays. Two different types of hydrogel beads were synthesized, both exhibiting high stability and easy operability. The hydrogel bead-based immunoassay method showed high sensitivity and specificity in the determination of acute myocardial infarction (AMI) biomarkers, with linear ranges and low detection limits. The results demonstrate the potential of these hydrogel beads as universal platforms for a wide range of CL immunoassays.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Rui Yang, Chuanbao Yuan, Wenxuan Dong, Yueran Ren, Yangyang Xue, Hua Cui
Summary: In this study, magnetic nanoparticles with excellent catalytic performance and fast magnetic separation ability were synthesized and used for constructing a label-free chemiluminescence immunosensor for antioxidant detection. The developed immunosensor exhibited a wide linear range and low detection limit, making it suitable for clinical diagnosis.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Fang Li, Yachao Xi, Jianming Jiang, Hao Peng, Bing Li, Jianbo He, Jiangnan Shu, Hua Cui
Summary: Graphitic carbon nitride (g-CN) with wavelength-tunable electrochemiluminescence (ECL) emission is synthesized in this study through the condensation of melamine using o-fluorobenzoic acid as a regulation reagent. The porous structure and nitrogen defects of the synthesized g-CN enable wavelength-tunable multicolor ECL emissions. A multicolor ECL sensing array is proposed for the discrimination of polyphenols using g-CN and porous g-CN as sensing materials. The study provides a simple strategy to adjust the ECL wavelength of g-CN and presents a simple way to fabricate multicolor ECL sensing array, with great potential for multiplexed analysis and multicolor ECL imaging sensing.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Tianhua Zheng, Wei Nie, Lu Yu, Jiangnan Shu, Yaohua Li, Changlin Tian, Wei Wang, Hua Cui
Summary: This study presents a chemical timer approach for manipulating the emission onset time of chemiluminescence (CL) reactions. By adding thiourea, the delay of CL emission can be achieved through a slow-generation-scavenging mechanism, which is applicable not only to CL reactions but also to other common chromogenic reactions. The precise regulation of CL kinetics can be utilized in dynamic chemical coding with improved coding density and security.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Fajin Qu, Jiangnan Shu, Shanshan Wang, Mohammad A. Haghighatbin, Hua Cui
Summary: In this study, nanocarriers were used to immobilize ABEI and Co2+, resulting in the synthesis of PMAANGs-ABEI/Co2+ with extraordinary CL performance. The nanogels exhibited excellent catalytic ability, CL stability, and were utilized in the development of an immunoassay for SARS-CoV-2 nucleocapsid protein.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Aihua Lyu, Yisha Wang, Hua Cui
Summary: In this study, a new mechanism for enhancing chemiluminescence (CL) was developed through the coembedding of N-(aminobutyl)-N-(ethylisoluminol) (ABEI) and Co2+ into gold nanoparticle-modified covalent-organic frameworks (COFs). The confinement of ABEI and Co2+ into Au-COF brought over 20-fold enhancement of CL intensity. This work proposes a novel enhancement mechanism for CL based on crystalline porous materials, which provides a new perspective for the development of CL-functionalized materials for biosensors and bioassays.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Yisha Wang, Jiangnan Shu, Aihua Lyu, Manli Wang, Chao Hu, Hua Cui
Summary: In this study, a simple and effective endogenous strategy of post-synthesis-modified Zn2+ was proposed to enhance electrochemiluminescence (ECL) emissions of nonmetal porphyrin-based metal-organic frameworks (MOFs). The enhanced ECL signal of Zn-TCPP/UiO-66-NH2 nanoluminophores was utilized to develop a label-free ECL biosensor for detecting nanoscale exosomes, which exhibited good sensitivity and a wide detection range. This work provides inspiration for exploring strategies to enhance ECL efficiencies of porphyrin-based MOFs, which has important application prospects in sensitive bioassays.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Analytical
Manli Wang, Jiangnan Shu, Shanshan Wang, Aihua Lyu, Yisha Wang, Dabing Huang, Hua Cui
Summary: A novel magnetic nanomaterial, Fe3O4@Au/Ag/ABEI/Co2+, with strong and stable chemiluminescence (CL) emission was synthesized. A label-free CL immunosensor for exosome detection was established based on this nanomaterial, exhibiting excellent analytical performance. The immunosensor has the potential for early cancer diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Shanshan Wang, Fajin Qu, Ruoxian Zhang, Tengchuan Jin, Tianhua Zheng, Jiangnan Shu, Hua Cui
Summary: Recently, a chemical timer approach to manipulate the onset time of chemiluminescence (CL) emission was developed. By modifying Mn(2+) on the surface of L012-protected gold nanoparticles, an emission onset time-adjustable chemiluminescent L012-Au/Mn2+ was synthesized, which could generate strong and long-time CL emission with adjustable onset time. This approach was applied to construct a label-free CL immunoassay for the high-throughput and sensitive determination of the severe acute respiratory syndrome coronavirus 2 nucleocapsid (N) protein, showing good linearity and low detection limit.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Wei Nie, Ruoxian Zhang, Chao Hu, Tengchuan Jin, Xi Wei, Hua Cui
Summary: A self-enhanced electrochemiluminescence array chip was developed for highly sensitive, label-free detection of SARS-CoV-2 nucleocapsid protein. The chip demonstrated excellent performance in rapid and accurate detection of viral antigens, and it can be easily operated with portable equipment.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Materials Science, Multidisciplinary
Mingquan Guo, Jiangnan Shu, Dexin Du, Yisha Wang, Hua Cui
Summary: The development of potential resolved electrochemiluminescence (ECL) nanoluminophores is crucial for precise and multiplex analysis. In this study, a new nanoluminophore TCPP@CRuSiO2 was designed and synthesized, which exhibited both cathode and anode ECL emissions. The nanoluminophores had a uniform sphere morphology with a diameter of around 50 nm. They generated two ECL emissions at different potentials using K2S2O8 and tripropylamine (TPrA) as coreactants. The ECL mechanisms were proposed based on the reactions between different species. The synthesized TCPP@CRuSiO2 nanoluminophores not only possessed potential and wavelength resolved ECL emissions, but also showed advantages of easy synthesis, high ECL efficiency, good stability, and easy film formation on the electrode surface. This work enriches the study of ECL nanoluminophores and has important potential applications in precise and multiplex analysis.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Chemistry, Multidisciplinary
Yaohua Li, Sa Wang, Xinyu He, Shijun Li, Tianhua Zheng, You-Peng Chen, Hua Cui, Wei Wang
Summary: The study utilized a monolayer of bioluminescent bacteria to visualize the dynamic distribution of oxygen, leading to the discovery of oxygen waves at the interface. Further investigation revealed that the oxygen waves in a microtiter plate were caused by micro-convections induced by inhomogeneous evaporation and thermal fluctuation.
Article
Chemistry, Analytical
Gen Chen, Tan He, Song Sun, Jun Bao, Hua Cui, Chen Gao
Summary: A fiber bundle-based chemiluminescence detection system was developed for simultaneous and efficient detection of multiple chemiluminescent samples. This system, utilizing the large numerical aperture and high transmittance of optical fibers, showed about a 50 times increase in chemiluminescence collection efficiency compared to traditional lens-based imaging systems. This work provides a promising method for multiple sample chemiluminescence detection and could find applications in bioassays.
ANALYTICAL METHODS
(2021)
