期刊
ANALYST
卷 138, 期 24, 页码 7294-7298出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3an01540h
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资金
- Engineering and Physical Sciences Research Council (EPSRC) [EP/I029036/1]
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Faculte des Sciences of the Universite du Quebec a Montreal
- Ministere de l'Education, du Loisir et du Sport du Quebec
- NSERC
- Centre Quebecois sur les Materiaux Fonctionnels (CQMF)
- Fonds de Recherche du Quebec - Nature et Technologies (FRQNT)
- Engineering and Physical Sciences Research Council [EP/H044795/1, EP/I029036/1] Funding Source: researchfish
- EPSRC [EP/I029036/1] Funding Source: UKRI
Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERG(S5-S6) can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.
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