期刊
ANALYST
卷 137, 期 14, 页码 3234-3237出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an16154k
关键词
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资金
- KAKENHI [22500448]
- Funding Program for World-Leading Innovative R&D on Science and Technology
- Grants-in-Aid for Scientific Research [23810034, 22500448] Funding Source: KAKEN
We present a new method for rapid microRNA detection with a small volume of sample using the power-free microfluidic device driven by degassed PDMS. Target microRNA was detected by sandwich hybridization taking advantage of the coaxial stacking effect. This method allows us to detect miR-21 in 20 min with a 0.5 mu L sample volume at a limit of detection of 0.62 nM. Since microRNAs can act as cancer markers, this method might substantially contribute to future point-of-care cancer diagnosis.
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