Review
Oncology
Robert H. Eibl, Markus Schneemann
Summary: Liquid biopsy has shown promising potential in the diagnosis, prognosis, monitoring, and therapy of brain tumors, with the ability to monitor tumor progression and treatment response based on genetic profiling. However, challenges and disappointments also exist, highlighting the urgent need for suitable biomarkers and standards to enhance modern clinical management of brain tumor patients.
Review
Genetics & Heredity
Bowen Jiang, Deqian Xie, Shijin Wang, Xiunan Li, Guangzhen Wu
Summary: During the last decade, non-invasive liquid biopsy methods have replaced traditional imaging and invasive pathological methods for cancer diagnosis. These methods enable early screening and diagnosis of solid tumors, as well as continuous monitoring of tumor progression. Recent advancements in isolation and analysis techniques have improved accuracy, expanded sample acquisition and target testing, and extended the detection of solid tumors from blood and other body fluids.
FRONTIERS IN GENETICS
(2023)
Review
Chemistry, Analytical
Joanna Bogusiewicz, Barbara Bojko
Summary: Brain tumor removal is an important step in therapeutic regimens. Accurate differentiation of cancerous and non-cancerous tissue, quick assessment of lesion types and grades, and real-time monitoring of tumors can improve surgical outcomes. Mass-spectrometry-based methods have shown promising results in these aspects.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Oncology
Malak Abedalthagafi, Nahla Mobark, May Al-Rashed, Musa AlHarbi
Summary: Brain tumors in children are different from those in adults, and recent research has identified key molecular and genetic changes in pediatric tumors. These findings have led to the development of novel epigenetic and immunotherapeutic treatment approaches that are currently being evaluated in clinical trials to better address pediatric brain tumors.
NPJ PRECISION ONCOLOGY
(2021)
Review
Pharmacology & Pharmacy
Gelare Ghajar-Rahimi, Kyung-Don Kang, Stacie K. Totsch, Sam Gary, Abbey Rocco, Sarah Blitz, Kara Kachurak, M. R. Chambers, Rong Li, Elizabeth A. Beierle, Asim Bag, James M. Johnston, James M. Markert, Joshua D. Bernstock, Gregory K. Friedman
Summary: Malignant brain tumors are a significant health issue in children and current treatment options have poor outcomes and long-term morbidity. Oncolytic virotherapy has emerged as a promising immunotherapy for pediatric brain tumors. This review focuses on past and current clinical trials using oncolytic virotherapy in pediatric brain tumors and discusses future research directions.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Pharmacology & Pharmacy
Yi Zhao, Ping Yue, Yao Peng, Yuanyuan Sun, Xing Chen, Ze Zhao, Bingjie Han
Summary: Brain tumor is a significant cause of mortality and poor survival rates, with various challenges in treatment due to the blood-brain barrier, tumor heterogeneity, and drug resistance. This review focuses on the challenges faced by multi-functional nano drug delivery systems in penetrating the blood-brain barrier and targeting cancer cells or organelles. It also discusses the progress in combining different therapies using multi-functional nano drug delivery systems for brain tumor treatment.
Article
Clinical Neurology
Coral M. Stredny, Melissa M. Blessing, Vivian Yi, Morgan E. Ryan, Bo Zhang, Isaac H. Solomon, Sanjay P. Prabhu, Sanda Alexandrescu, Mark P. Gorman
Summary: This study emphasizes the importance of pursuing non-invasive testing before performing a brain biopsy for suspected small vessel primary angiitis of the central nervous system.
ANNALS OF NEUROLOGY
(2023)
Article
Spectroscopy
M. Kopec, M. Blaszczyk, M. Radek, H. Abramczyk
Summary: This study evaluates the Raman biomarkers to distinguish tumor types, demonstrating that specific frequency bands in the spectra can serve as universal biomarkers for assessing the aggressiveness of human brain tumors. The clinical potential of Raman spectroscopy in oncological diagnostics is confirmed by the presented results.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Oncology
Agostino Chiaravalloti, Maria Ricci, Andrea Cimini, Francesca Russo, Francesco Ursini, Luca Filippi, Orazio Schillaci
Summary: This study aims to assess the correlation between SUV-derived indices and volumetric uptake parameters in 18F-FDOPA PET/CT for primary brain tumors. The results demonstrate that volumetric uptake parameters are easier to assess in primary brain tumors with higher SUV max and SUV max ratios, highlighting the emerging role of volumetric parameters in data interpretation.
Article
Oncology
Matyas Bukva, Gabriella Dobra, Juan Gomez-Perez, Krisztian Koos, Maria Harmati, Edina Gyukity-Sebestyen, Tamas Biro, Adrienn Jenei, Sandor Kormondi, Peter Horvath, Zoltan Konya, Almos Klekner, Krisztina Buzas
Summary: The study explored the potential role of serum-derived small extracellular vesicles (sEVs) in diagnosing CNS tumors through Raman spectroscopic analyses. The results showed that Raman spectroscopic analysis of sEV-sized particles could distinguish different tumor types with high sensitivity and specificity, suggesting it is a promising liquid-biopsy-based method for CNS tumor diagnosis.
Review
Oncology
Alessia Pellerino, Priscilla K. Brastianos, Roberta Ruda, Riccardo Soffietti
Summary: Leptomeningeal metastases from solid tumors are a devastating complication with poor survival, diagnosed through neurological examination, cerebrospinal fluid cytology, and brain/spinal MRI. Novel techniques like liquid biopsy aim to enhance detection sensitivity and specificity.
Review
Oncology
Zhenjie Yi, Lifu Long, Yu Zeng, Zhixiong Liu
Summary: Radiomics plays a crucial role in extracting quantitative features from complex clinical imaging arrays to study their relevance with tumor histological features. Recent studies have shown broad applications of radiomics in identifying primary tumors, conducting differential diagnosis, grading, evaluating mutation status and aggression, predicting treatment response and recurrence.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Grazia Menna, Giacomo Piaser Guerrato, Lal Bilgin, Giovanni Maria Ceccarelli, Alessandro Olivi, Giuseppe Maria Della Pepa
Summary: The lack of studies on liquid biopsy for brain tumors indicates that it is currently not widely used in the field of central nervous system cancers. This systematic review focuses on the application of machine learning to liquid biopsy for brain tumors, aiming to provide practical guidance for neurosurgeons and explore potential challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Polymer Science
Jisan Ahamed, B. H. Jaswanth Gowda, Waleed H. Almalki, Neelima Gupta, Amirhossein Sahebkar, Prashant Kesharwani
Summary: Malignant brain tumors have a poor prognosis and low survival rate due to the challenges in drug delivery, including crossing the blood-brain barrier, achieving optimal drug distribution within the tumor, and minimizing damage to healthy cells. Nanotechnology has emerged as a promising approach for brain tumor treatment by using nanoparticles to overcome these obstacles.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Clinical Neurology
Shiva Bayat, Ali Mamivand, Alireza Khoshnevisan, Abolfazl Maghrouni, Sasan Shabani, Mohammad-Taghi Raouf, Mehdi Yaseri, Hiva Saffar, Mina Tabrizi
Summary: The study found higher expression of HIF1a in glioma samples compared to meningioma, and higher expression levels of YAP1 and GPRC5A in meningioma. GPRC5A, HIF1a, and YAP1 showed acceptable power for differential diagnosis of glioma from meningioma.
WORLD NEUROSURGERY
(2021)
Review
Biochemical Research Methods
Ghayathri Balasundaram, Christoph Krafft, Ruochong Zhang, Kapil Dev, Renzhe Bi, Mohesh Moothanchery, Juwrgen Popp, Malini Olivo
Summary: Breast conserving surgery is popular but incomplete tumor removal may require reoperation. Biophotonic technologies are evolving to be an intraoperative tool for assessing breast cancer surgical margins.
JOURNAL OF BIOPHOTONICS
(2021)
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Joao L. Lagarto, Enrico Baria, Melis Goktas, Patrick Igoche Onoja, Kerstin G. Blank, Francesco S. Pavone, Juergen Popp, Christoph Krafft, Riccardo Cicchi
Summary: The study demonstrates the potential of nondestructive optical imaging techniques such as SHG, RS, and FLIM in monitoring collagen degradation and assessing mechanical properties in tissues. These techniques can provide label-free monitoring of collagen degradation and rough evaluation of mechanical properties in a nondestructive manner.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Eric Boateng Osei, Liliia Paniushkina, Konrad Wilhelm, Juergen Popp, Irina Nazarenko, Christoph Krafft
Summary: Extracellular vesicles (EVs) ranging in size from 60 to 800 nm were isolated from plasma of control and prostate cancer patients using differential centrifugation and ultrafiltration techniques. Spontaneous Raman and surface-enhanced Raman spectroscopy (SERS) were employed to identify fractions that best discriminate between control and cancer patients, with results showing that EV12 and EV120 fractions provide the most effective separation.
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Enrico Baria, Xinyue Wang, Florian Korinth, Joao L. Lagarto, Christiane Hoeppener, Francesco S. Pavone, Volker Deckert, Juergen Popp, Riccardo Cicchi, Christoph Krafft
Summary: This study investigated the biochemical, structural, and biomechanical effects of genipin (GE) cross-linking on equine pericardium (EP) using optical imaging techniques and atomic force microscopy (AFM). Results showed that GE cross-linking led to a decrease in second-harmonic signal and an increase in autofluorescence, indicating changes in biochemical and structural properties. Additionally, cross-linking increased the Young's modulus of EP.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Dana Cialla-May, Christoph Krafft, Petra Roesch, Tanja Deckert-Gaudig, Torsten Frosch, Izabella J. Jahn, Susanne Pahlow, Clara Stiebing, Tobias Meyer-Zedler, Thomas Bocklitz, Iwan Schie, Volker Deckert, Juergen Popp
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Uta Barbara Metzing, Christian von Loeffelholz, Ricardo Steidl, Bernd Romeike, Rene Winkler, Falk Rauchfuss, Utz Settmacher, Christian Stoppe, Sina M. Coldewey, Claudia Weinmann, Martin O. Weickert, Ralf A. Claus, Andreas L. Birkenfeld, Christian Kosan, Paul Horn
Summary: This study provides a descriptive characterization of the unfolded protein response (UPR) in skeletal muscle of human patients with peritoneal sepsis and a sepsis model of C57BL/6J mice. The expression patterns of UPR and inflammatory injury markers were found to be different in patients and mice with sepsis.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Karina Kocot, Katarzyna Pytlakowska, Ewa Talik, Christoph Krafft, Rafal Sitko
Summary: In this study, ss-cyclodextrin/graphene oxide (GO-ss-CD) was used as an effective adsorbent for dispersive micro-solid phase extraction (DMSPE) of uranyl ions (UO22+) from water samples. The adsorption capacity and resistance to high ionic strength make it a potential method for preconcentration of UO22+ ions. The accuracy of the method was verified by analyzing certified reference material and spiked water samples.
Article
Multidisciplinary Sciences
Julia Sophie Boeke, Juergen Popp, Christoph Krafft
Summary: This study utilized O-PTIR technology for the detection and identification of microplastic particles, analyzing the spectra of various plastic materials and introducing a novel two-dimensional identification method for better recognition of microplastic particles in environmental and biological samples.
SCIENTIFIC REPORTS
(2022)
Review
Optics
Christoph Krafft, Jurgen Popp
Summary: Modern optical and spectral technologies have the potential to characterize tissues at a molecular level, enabling the identification of pathological tissues and the grading and staging of tumors without the need for labeling. The current tools for intraoperative tumor assessment, as well as the requirements for tissue visualization from a medical and optical perspective, are described. Approaches utilizing optical and spectral techniques for ex vivo and in vivo monitoring are introduced, and proof-of-concept studies utilizing linear and nonlinear spectroscopy and imaging modalities are presented. The use of modern artificial intelligence and deep learning concepts to analyze spectroscopic and imaging datasets has contributed to the advancements in each technique. Finally, the article provides an outlook on the opportunities and prospects of clinical translation.
Article
Oncology
Christoph Krafft, Juergen Popp, Peter Bronsert, Arkadiusz Miernik
Summary: Raman spectroscopy allows for label-free assessment of bladder tissue in intraoperative applications. In a retrospective study, Raman microspectroscopic images were used to identify different components of bladder tissue, including normal bladder, bladder cancer, necrosis, epithelium, and lipid inclusions. Additionally, foreign materials such as microplastic particles and pigments were detected in the specimens. This study demonstrates the potential of Raman spectroscopy for tumor detection and material identification in complex matrices.
Article
Chemistry, Analytical
Tanveer Ahmed Shaik, Anuradha Ramoji, Nils Milis, Juergen Popp, Christoph Krafft
Summary: This study demonstrates how the O-PTIR technique can overcome the limitations of direct mid-infrared spectroscopic imaging of single cells in an aqueous buffer. By using an indirect detection principle, the technique achieves high-resolution discrete wavenumber imaging and fingerprint spectroscopy of cultivated cells. The study also shows the potential applications of O-PTIR in high-content screening and subcellular analysis.
Article
Education, Scientific Disciplines
Christin Kleinsorgen, Andrea Baumann, Barbara Braun, Jan Griewatz, Johannes Lang, Holger Lenz, Johanna Mink, Tobias Raupach, Bernd Romeike, Thomas Christian Sauter, Achim Schneider, Daniel Tolks, Inga Hege
Summary: This review paper examines the development and perspectives of digital teaching, learning, and assessment in the GMS Journal for Medical Education (GMS JME). Through the analysis of 132 articles, it was found that the majority of studies focused on virtual patients or case-based learning, while no articles were published on trends such as serious games or virtual reality. The analysis also reveals a decline in the breadth of research on digital teaching after 2010.
GMS JOURNAL FOR MEDICAL EDUCATION
(2022)
Article
Chemistry, Physical
Stefan Kranz, Markus Heyder, Stephan Mueller, Andre Guellmar, Christoph Krafft, Sandor Nietzsche, Caroline Tschirpke, Volker Herold, Bernd Sigusch, Markus Reise
Summary: The study investigated the effect of a zinc carbonate-hydroxyapatite-containing dentifrice on demineralized enamel and dentin surfaces. The results showed no significant differences in enamel surfaces, but a reduction in roughness and minor mineral precipitation on dentin surfaces.
Article
Spectroscopy
Thomas G. Mayerhoefer, Christoph Krafft
Summary: The spectroscopic literature often suggests that peak shifts are generally correlated with structural changes on the molecular or unit cell level, but there are also situations where peaks shift for reasons unrelated to chemical structural changes. To avoid misinterpretations, practitioners should be aware of these reasons.
Article
Chemistry, Analytical
Florian Korinth, Tanveer Ahmed Shaik, Jurgen Popp, Christoph Krafft
Summary: The study utilized the SERDS technique to process GE-crosslinked EP samples, revealing that the difference spectra generated by SERDS had clearer collagen and GE pigment bands with lower standard deviation, and could be applied to Raman studies on samples with strong fluorescence backgrounds.