期刊
ANALYST
卷 137, 期 23, 页码 5571-5575出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an36011j
关键词
-
资金
- National Natural Science Foundation of China (NSFC) [20735001, 20975003, 21175006]
- National High Technology Research and Development Program of China [2009AA035200]
A fast, simple and quantitative approach was established for monitoring autophagy in HeLa cells by directly detecting the conversion of green fluorescent protein (GFP) labelled autophagy markers, GFP-LC3-I to GFP-LC3-II, in crude cellular extract using capillary electrophoresis (CE) with laser-induced fluorescence (LIF). Compared with the traditional methods, this proposed method is simpler and more reliable. Moreover, high sensitivity, with a limit of detection (LOD) of 0.48 ppt (bovine serum albumin as standard protein), was obtained by an in-capillary derivatization method coupled with a field-enhanced sample injection (FESI) technique, this may allow the success of this technique in the detection of endogenous markers of autophagy in cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据