期刊
ANALYST
卷 137, 期 10, 页码 2415-2420出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2an35255a
关键词
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资金
- National Natural Science Foundation of China [21075100]
- Ministry of Education of China [708073]
- Specialized Research Fund for Doctoral program of Higher Education [20100182110015]
- Natural Science Foundation of Chongqing City [CSTC-2004BB4149, 2005BB4100]
- State Key Laboratory of Electroanalytical Chemistry (SKLEAC) [2010009]
- High Technology Project Foundation of Southwest University [XSGX 020]
Herein, we successfully fabricated a highly sensitive label-free electrochemical aptasensor for thrombin based on the amplification of graphene (Gra). The excellent electrochemical probe of nickel hexacyanoferrate nanoparticles (NiHCFNPs) was introduced to form Nafion-Graphene-NiHCFNPs (Nf-Gra-NiHCFNPs) nanocomposites membrane on the gold electrode. The employment of graphene not only enhanced the surface area of the electrode with increased NiHCFNPs immobilization, but also improved the conductivity of the electrode, which further effectively improved the sensitivity of this proposed aptasensor. Subsequently, AuNPs layer was formed to immobilize the thrombin aptamer (TBA) and enhance the stability of the composite monolayer mentioned above. Then, thiol-modified TBA was assembled onto the AuNPs layer. Thereafter, hexanethiol (HT) was employed to block the possible remaining active sites. With the dual amplification of Gra and AuNPs, the resulting aptasensor exhibited good current response to target thrombin with a wide linear range extended from 1 pM to 80 nM (the detection limit was 0.3 pM). Additionally, the morphologies of bare Au substrate, nickel hexacyanoferrate nanoparticles (NiHCFNPs) and nanocomposites were successfully characterized by atomic force microscopy (AFM).
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