Article
Agriculture, Multidisciplinary
Randy W. Purves, Kim Souster, Michelle West, Azhar M. Huda, Caleb M. E. Fisher, Michael W. Belford, Bryn O. Shurmer
Summary: Thyreostatic drugs are illegally used in animals for food production, leading to lower quality meat and potential adverse effects on humans. Detecting these drugs in animal tissues can be challenging due to their low-molecular-weight properties. The study proposes an improved LC-FAIMS-SRM method, which combines liquid chromatography with high-field asymmetric waveform ion mobility spectrometry and selective reaction monitoring. This method demonstrates significant improvements in signal-to-noise ratio and lower limit of quantification compared to the traditional LC-SRM method.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Medicine, Legal
Asli Atasoy, Nebile Daglioglu, Ismail Ethem Goren, Alperay Girisbay, Rukiye Aslan, Serap Annette Akgur
Summary: Synthetic cannabinoids pose a significant public health and safety issue as their ever-changing structures complicate drug tests and most cannot be detected by routine screening tests, leading to increased usage. A study found that immunoassay screening tests were insufficient and validated chromatographic methods should be used for clinical and forensic cases to accurately detect synthetic cannabinoids in urine samples.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Marina Arenas, Julia Martin, Juan Luis Santos, Irene Aparicio, Omar Fernandez-Sanfrancisco, Esteban Alonso
Summary: This study optimized four techniques for the determination of platinum-based cytostatic drugs in urine samples. LC-MS/MS method exhibited the best analytical features. The selected method was successfully applied for the quantification of platinized cytostatic drugs in urine samples from oncologic patients.
Article
Biochemical Research Methods
Mark Timms, Rohan Steel
Summary: The proteotypic human EPO peptides can be used to confirm the presence of rhEPO in equine samples, but caution is needed due to possible false identification caused by isomeric leucine or isoleucine amino acids. Confirming rhEPO in equine samples through immuno-affinity capture and LC-MS/MS analysis is accurate. Chromatography plays a crucial role in determining LC-MS/MS specificity, and the accuracy of peptide identification can be enhanced by including labeled peptide internal standards.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Shota Yoshida, Hongyan Zhang, Ryuji Takahashi, Sakura Yoshida, Yumi Abiko, Akira Toriba
Summary: This study identified non-polar compounds that coprecipitate with aflatoxins and developed an effective pretreatment method combining zirconia-coated silica gel and IAC. The method has been successfully applied for the quantification of aflatoxins in four types of samples and in real samples where mold growth was encouraged.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
B. Choi, J. H. Kim, K. S. Lee, C. Kim, J. Y. Lee, H. M. Park
Summary: This study aimed to validate the analytical method for detecting multiple mycotoxins in roasted coffee samples. The method used liquid chromatography-tandem mass spectrometry and an immunoaffinity column for sample pre-treatment. The results showed that the validated method was suitable for multimycotoxin detection in roasted coffee samples.
INTERNATIONAL FOOD RESEARCH JOURNAL
(2023)
Review
Chemistry, Analytical
Gabriel Davi Marena, Matheus Aparecido dos Santos Ramos, Tais Maria Bauab, Marlus Chorilli
Summary: This review article collects important information on the physical, chemical, and biological properties of Amphotericin B (AmB) as well as the main analytical methods for quantifying and determining it. The article provides the scientific community with important information for choosing the best analysis method and ensuring the reliability of research results.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Pritam D. Sonawane, Suraj R. Chaudhari, Saurabh B. Ganorkar, Amod S. Patil, Atul A. Shirkhedkar
Summary: This review provides an overview of critical analytical aspects of quantifying AMB in pharmaceutical and biological samples. The review discusses various analytical methods and the physicochemical properties of AMB, offering important background information for further research.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Wei-Jay Chen, Ren-Jang Wu, Hsueh-Hui Yang, Ahai-Chang Lua, Yin-Jeh Tzeng, Shun-Hsing Tuan, Liang Chen, Tsong-Yung Chou
Summary: The abuse of PMMA and MDMA among young people is a growing public health problem. A simple method for simultaneous enantiomeric determination of PMMA and MDMA has been developed, which is rapid, environmentally friendly, and safe.
Article
Chemistry, Applied
Huamei Yang, Xiaoli Jiang, Yanli Wang, Chen Li, Li Hang, Weihong Huang
Summary: A new UPLC-ESI-MS/MS method was developed for the accurate determination of semicarbazide in human urine, showing high sensitivity and reproducibility. Residues of semicarbazide in children's 24-h urine samples were found to range from 1.0 to 41.5 µg/L.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Biochemical Research Methods
Stephan Eckert, Yun-Chien Chang, Florian P. Bayer, Matthew The, Peer-Hendrik Kuhn, Wilko Weichert, Bernhard Kuster
Summary: The study evaluated the use of nLC-FAIMS-MS/MS for analyzing FFPE tissue samples, showing comparable proteomic depth and reproducibility to offline fractionation with StageTip-based basic pH reversed-phase, but with 50% less sample required and significantly reduced sample handling time.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Genetics & Heredity
Hao Liu, Jiang Zhu, Qiu Li, Dongjuan Wang, Kexing Wan, Zhaojian Yuan, Juan Zhang, Lin Zou, Xiaoyan He, Jingkun Miao
Summary: Metabolomics was used to successfully differentiate IMDs patients from controls, revealing a broader metabolic spectrum in patients. Several potential novel markers were identified, and the metabolic pathways of IMDs were explored in depth.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2021)
Article
Toxicology
Selina Hemmer, Lea Wagmann, Markus R. Meyer
Summary: The study utilized untargeted metabolomics to investigate the acute effects of amphetamine on the plasma and urinary metabolome in rats. It revealed a downregulation of amino acids in plasma samples after amphetamine exposure, and identified four new potential biomarkers in urine. The research highlights the need for further studies to elucidate altered metabolic pathways associated with acute amphetamine exposure.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Food Science & Technology
Joao V. B. Borsatto, Edvaldo V. S. Maciel, Alejandro Cifuentes, Fernando M. Lancas
Summary: Sample preparation is a complex and time-consuming procedure that directly affects analysis quality. Recent studies have explored online extraction coupled with liquid chromatography-mass spectrometry (OLE-LC-MS) as a promising alternative for analyzing analytes in complex solid matrices. In this study, the lecithin profile in various seeds was analyzed using OLE-LC-MS, demonstrating its feasibility without the need for offline solvent extraction. Optimization of the extraction phase gradient method and exploration of different analytical columns improved separation and repeatability. Overall, the OLE-LC-MS approach showed potential for lipidomics analysis in the future.
Article
Biochemical Research Methods
Carolina Turnes Pasini Deolindo, Cristian Rafael Kleemann, Cristina Bosch-Orea, Luciano Molognoni, Heitor Daguer, Rodrigo Barcellos Hoff, Ana Carolina Oliveira Costa
Summary: Lipophilic marine biotoxins (LMBs) pose a significant risk to the consumption of mussels and oysters. This study demonstrates the use of incurred samples for optimization, validation, and monitoring of a fast ultrasound-assisted extraction (UAE) procedure. An internal quality control material containing okadaic acid was produced and included in all analytical routines. Additionally, a sample pooling protocol reduced instrument analysis time by up to 80%.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Letter
Biochemical Research Methods
Agnes Barnabe, Yves Moulard, Stephane Trevisiol, Sophie Boyer, Mylene Caroff, Wafek Taleb, Sophie Tendon, Laura Drif, Vivian Delcourt, Marie-Agnes Popot, Ludovic Bailly-Chouriberry
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Stephane Trevisiol, Yves Moulard, Vivian Delcourt, Murielle Jaubert, Sophie Boyer, Sophie Tendon, Hayate Haryouli, Wafek Taleb, Mylene Caroff, Benjamin Chabot, Laura Drif, Francois Andre, Patrice Garcia, Benoit Loup, Marie-Agnes Popot, Ludovic Bailly-Chouriberry
Summary: According to international sports institutions, the use of PPAR-delta agonist GW501516 is forbidden for athletes due to its performance-enhancing capabilities. However, it is easily accessible online, posing a challenge for doping control efforts. Urine testing is preferred for its non-invasive nature and wider detection window, but the native form of GW501516 is often undetectable due to metabolism.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Benoit Loup, Francois Andre, Justine Avignon, Marion Lhuaire, Vivian Delcourt, Agnes Barnabe, Patrice Garcia, Marie-Agnes Popot, Ludovic Bailly-Chouriberry
Summary: This study is the first exploration of detecting circulating miRNAs in plasma dedicated to equine doping control, using gene expression analysis to develop a new method for detection and providing a new approach for detecting doping abuse.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Patrice Garcia, Isabelle Perot, Benoit Loup, Frederic Balssa, Murielle Jaubert, Vivian Delcourt, Chris Dujardin, Marie-Agnes Popot, Ludovic Bailly-Chouriberry
Summary: Clodronate, a non-nitrogen-containing bisphosphonate drug, is used in equine veterinary medicine but prohibited in competition horses. A quantitative method using high-performance liquid chromatography-tandem mass spectrometry was developed for detection in plasma, with good accuracy and stability.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Chloe Cloteau, Gaud Dervilly, Zied Kaabia, Florian Bagilet, Vivian Delcourt, Benoit Loup, Yann Guitton, Anne-Lise Royer, Fabrice Monteau, Patrice Garcia, Marie-Agnes Popot, Bruno Le Bizec, Ludovic Bailly-Chouriberry
Summary: A non-targeted metabolomics approach was used to identify potential biomarkers associated with the abuse of Anabolic-Androgenic Steroids in animal competitions using equine urine samples. The model's performances and robustness were successfully preserved in a high-throughput liquid chromatography-selected reaction monitoring method. The transferability of the method to a second laboratory demonstrated its applicability in developing biomarker-based strategies for doping control.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Marjaana Viljanto, Zied Kaabia, Polly Taylor, Tessa Muir, Jocelyn Habershon-Butcher, Ludovic Bailly-Chouriberry, James Scarth
Summary: This study investigated the presence of six steroids in the postrace urine of castrated male horses and found that Boldenone was detected at low concentrations in all samples. The study also found that Boldienone, testosterone, androstenedione, Delta 1-progesterone, and 20(S)-hydroxy-Delta 1-progesterone undergo ex vivo transformation after urine samples are stored at room temperature for 7 days. Therefore, a biomarker approach using Delta 1-progesterone and 20(S)-hydroxy-Delta 1-progesterone concentrations is proposed to differentiate steroid administration and ex vivo transformation.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Stephane Trevisiol, Yves Moulard, Zied Kaabia, Vivian Delcourt, Benoit Loup, Patrice Garcia, Sophie Boyer, Karine Dauriac, Guillaume Groseille, Sebastien Rouger, Ruediger Narbe, Marie-Agnes Popot, Ludovic Bailly-Chouriberry
Summary: Ciclesonide is a highly potent inhaled corticosteroid used for the treatment of asthma in humans and horses. Due to its anti-inflammatory properties, it is prohibited for use in horse competitions, necessitating the development of sensitive analytical methods for detection in plasma.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Vivian Delcourt, Patrice Garcia, Benjamin Chabot, Agnes Barnabe, Maelle Bouscarel, Benoit Loup, Marie-Agnes Popot, Ludovic Bailly-Chouriberry
Summary: Currently, many websites sell products online without approval from EMA or FDA, which may pose risks due to lack of regulation and official approval. In this study, we analyze three misbranded and adulterated products named TB500, TB1000, and SGF1000. Results show that the content of TB500/TB1000 products is inconsistent with their descriptions, and SGF1000 is primarily composed of sheep ECM and blood proteins, with diluted growth promoters.
DRUG TESTING AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Justine Pinetre, Vivian Delcourt, Francois Becher, Patrice Garcia, Agnes Barnabe, Benoit Loup, Marie-Agnes Popot, Francois Fenaille, Ludovic Bailly-Chouriberry
Summary: In the past decade, many innovative biotherapeutics have been marketed. Monoclonal antibodies and Fc-fusion proteins have been developed as targeted therapies for the treatment of various diseases. However, the misuse of these biomolecules as performance enhancers raises concerns. A new screening method using UHPLC-HRMS/MS has been developed for the analysis of mAbs and related macromolecules in equine plasma. This method demonstrates reliable performances at low concentrations with high-throughput capability, enabling the detection of human biotherapeutics in horses.
DRUG TESTING AND ANALYSIS
(2023)
Article
Biochemical Research Methods
Marjaana Viljanto, Zied Kaabia, Polly Taylor, Pamela Hincks, Tessa Muir, Jocelyn Habershon-Butcher, Ludovic Bailly-Chouriberry, James Scarth
Summary: Boldenone, a prohibited substance in equine sports, can be endogenous and potentially formed by microbial activity in horse urine. The concentrations of biomarkers Delta 1-progesterone, 20(S)-hydroxy-Delta 1-progesterone, and 20(S)-hydroxyprogesterone were monitored alongside boldenone in untreated female horse urine. A differentiation approach was developed based on the detection of these biomarkers, and it was found that the presence of certain biomarkers could indicate ex vivo transformation or consumption of altered feed rather than steroid administration.
DRUG TESTING AND ANALYSIS
(2023)