期刊
ANALYST
卷 137, 期 3, 页码 680-685出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1an15942a
关键词
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资金
- Natural Science Research Foundation of China [21175058, 51003039]
- Natural Science Foundation of Shandong Province, China [ZR2011BQ019]
- Excellent Young and Middle-Aged Scientist Reward Foundation of Shandong Province [2008BS09006]
- Technology Development Plan of Shandong Province, China [2011GGB01153, 2009GG20003022]
In this work, we reported a simple and sensitive sandwich-type electrochemiluminescence (ECL) immunosensor for carcinoembryonic antigen (CEA) on a gold nanoparticles (AuNPs) modified glassy carbon electrode (GCE). The Ru-silica (Ru(bpy)(3)(2+)-doped silica) capped nanoporous gold (NPG) (Ru-silica@NPG) composite was used as an excellent label with amplification techniques. The NPG was prepared with a simple dealloying strategy, by which silver was dissolved from silver/gold alloys in nitric acid. The primary antibody was immobilized on the AuNPs modified electrode through L-cysteine and glutaraldehyde, and then the antigen and the functionalized Ru-silica@NPG composite labeled secondary antibody were conjugated successively to form a sandwich-type immunocomplex through the specific interaction. The concentrations of CEA were obtained in the range from 1 pg mL(-1) to 10 ng mL(-1) with a detection limit of 0.8 pg mL(-1). The as-proposed ECL immunosensor has the advantages of high sensitivity, specificity and stability and could become a promising technique for tumor marker detection.
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