期刊
ANALYST
卷 136, 期 8, 页码 1686-1689出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0an00977f
关键词
-
资金
- NSF [0700659]
- Directorate For Engineering [1202857] Funding Source: National Science Foundation
Vapor-liquid-solid (VLS) grown silica nanowires (SiO(2)NWs) have been deposited electrophoretically on a gold electrode and utilized for covalent immobilization of glucose oxidase (GOx). Covalent binding has been achieved via 3-aminopropyltriethoxysilane (APTES) modification and N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. Scanning electron microscopy, transmission electron microscopy and cyclic voltammetry techniques have been used to characterize SiO2NW and GOx/APTES/SiO2NW/Au bioelectrode. Electrochemical studies reveal that SiO2NW increases the effective electro-active surface area thus resulting in higher loading of enzyme. Response characteristics show linearity in the range of interest 25-300 mg dl(-1), with a detection limit of 11 mg dl(-1), sensitivity: 0.463 mu A (mg dl(-1))(-1) and regression coefficient of 0.992.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据