Article
Chemistry, Multidisciplinary
Xuancheng Fu, Wei Dong, Chang Liu, Jing Han, Chengzhi Huang
Summary: A label-free fluorescence resonance energy transfer (FRET) platform based on cationic conjugated polymers and aptamers was developed for ultrasensitive and specific ractopamine detection. The method exhibited a wide linear range from 0.05 to 500 μM and a low limit of detection of 47 nM, making it an attractive assay platform for foodborne doping.
Article
Chemistry, Analytical
Pengbo Zhang, Ke Qin, Anand Lopez, Zhengping Li, Juewen Liu
Summary: In this study, a cationic conjugated polymer (CCP)-based binding assay was developed for aptamer binding. The conformational change of aptamer after binding with a target was reflected by the fluorescence change of the CCP. Four aptamers for different targets were characterized, and the longer stem of the stem-loop structured aptamer facilitated target binding and enlarged the detection signal. This label-free and cost-effective method showed high specificity and sensitivity, making it a useful tool for aptamer binding evaluation and target detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Runfang Hao, Yangfan Yue, Leilei Li, Jianlong Ji, Qiang Zhang, Lifeng Ding, Shengbo Sang, Qiang Li
Summary: This paper presents a simple and inexpensive method for label-free detection of IgG using an active dielectric bilayer field effect transistor, showing high selectivity and broad commercialization prospects.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xi Zhou, Doudou Zhang, Ying Yan, Hailun He, Yukui Zhou, Changbei Ma
Summary: A label-free fluorescent method for detecting glutathione (GSH) based on a thioflavin T/G-quadruplex conformational switch is developed, showing high sensitivity and selectivity. The method can also be applied to detect other amino acids with satisfactory efficacy.
Article
Chemistry, Multidisciplinary
Pengfei Fan, Can Liu, Congcong Hu, Feifei Li, Xi Lin, Fubing Xiao, Hao Liang, Le Li, Shengyuan Yang
Summary: N,S-CDs with strong orange fluorescence were synthesized through a hydrothermal method, showing excitation-independent photoluminescent behavior and excellent stability. A label-free and sensitive detection method for VB12 in drugs was established based on the inner filter effect between VB12 and N,S-CDs.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Analytical
Cong Zheng, Da Wu, Tao Wang, Jianhong Xiao, Li Yu
Summary: This study developed a novel colorimetric method for detecting hydrogen peroxide (H2O2) and glucose in sodium chloride solutions, based on gold nanoparticles, single-stranded DNA, and ceria nanoparticles. The method can detect the target substances by observing the color change of the aqueous solution, providing a potential avenue for quantitative detection in clinical diagnostics.
Article
Chemistry, Analytical
Jing-Lin He, Ling Tang, Shi-Qing Liao, Mei-Tong Guo, Ling Wu, Yinghui Song, Sulai Liu, Zhong Cao
Summary: A label-free platform utilizing target-triggered hybridization and inhibition of gold nanoparticle aggregation was developed for naked-eye detection of telomerase activity. This method offers a cost-effective approach for cancer diagnosis and identification of breast cancer phenotypes.
Article
Chemistry, Analytical
Junhyun Park, Hyogu Han, Chihyun Park, Jun Ki Ahn
Summary: In this study, a personal glucose pyrophosphatase (PPase) detection method is demonstrated using cascade enzymatic reaction (CER) promoted by hexokinase and pyruvate kinase. Compared with previous methods, this method eliminates the need for expensive equipment and complex procedures, and allows quantitative evaluation of PPase activity within a dynamic linear range.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xueyun Chen, Liu Wang, Fang He, Ganghui Chen, Linlin Bai, Kaiyu He, Fang Zhang, Xiahong Xu
Summary: A novel label-free, colorimetric method for visual detection of V. parahaemolyticus was proposed, utilizing CRISPR/Cas12a to trim G-quadruplex DNAzyme and prevent color development of ABTS(2-). The method showed sensitivity comparable to real-time LAMP, detecting 6.1 x 10^2 CFU/mL V. parahaemolyticus in shrimp samples, making it a promising universal biosensing strategy for field pathogenic bacterial testing.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jan Oscar Cross-Zamirski, Elizabeth Mouchet, Guy Williams, Carola-Bibiane Schonlieb, Riku Turkki, Yinhai Wang
Summary: Cell Painting is a detection method used in drug discovery to predict bioactivity, assess toxicity, and understand the mechanisms of chemical and genetic disruptions. This study investigates label-free Cell Painting and finds that it can predict five fluorescent channels from brightfield input. Deep learning models were trained and validated, achieving high correlation and accuracy in predicting image features. The label-free Cell Painting method also shows potential for toxicity analysis and repurposing imaging channels for targeted biological research.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Yue Guan, Junning Ma, Jing Neng, Bolei Yang, Yan Wang, Fuguo Xing
Summary: In this study, a novel and label-free chemiluminescence aptasensor was constructed for rapid and accurate detection of the mycotoxin Zearalenone (ZEN). The aptasensor, based on a ZEN aptamer and a G-quadruplex DNAzyme, exhibited a perfect linear relationship for ZEN detection within a concentration range of 1-100 ng/mL, with a detection limit of 2.85 ng/mL. The aptasensor also showed satisfactory recovery rates for ZEN-spiked wheat and maize samples. Overall, the label-free chemiluminescence aptasensor displayed simplicity, sensitivity, specificity, and practicality in real samples, holding high application prospects in the food supply chain.
Article
Engineering, Electrical & Electronic
Massood Tabib-Azar, Braden Aaron Brown
Summary: Ionization spectra can be used to detect airborne and saliva SARS-COV-2 viruses without labels, and the technique shows potential for point-of-care diagnostic and testing applications.
IEEE SENSORS JOURNAL
(2022)
Article
Chemistry, Analytical
Jugal Suthar, Beatriz Prieto-Simon, Gareth R. Williams, Stefan Guldin
Summary: Extracellular vesicles, particularly exosomes, play a crucial role in intercellular communication and disease propagation. A novel biosensing platform, electrochemical quartz crystal microbalance with dissipation monitoring (EQCM-D), is demonstrated for dual-mode and label-free detection of plasma exosomes. By combining QCM-D with in situ electrochemical impedance spectroscopy measurement, relationships between mass, viscoelasticity, and impedance properties of each functional layer and analyte are revealed. This platform offers improved sensing specificity and lower limit of detection, making it an important technique for exosome analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Shaoguang Li, Huilin Liu, Xiaoying Zhang, Jiasong Cai, Kunlong Huang, Bing Chen, Liying Huang, Zhen Lin, Xinhua Lin, Hong Yao
Summary: The label-free colorimetric assay developed for detecting GSH utilizes the oxidation of OPD by Au3+ in the presence of GSH, leading to a decrease in absorbance intensity which can be used for quantification. This rapid and sensitive method has a wide detection range and can be easily conducted for the determination of GSH in biological fluids.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Cell Biology
YoungJu Jo, Hyungjoo Cho, Wei Sun Park, Geon Kim, DongHun Ryu, Young Seo Kim, Moosung Lee, Sangwoo Park, Mahn Jae Lee, Hosung Joo, HangHun Jo, Seongsoo Lee, Sumin Lee, Hyun-seok Min, Won Do Heo, YongKeun Park
Summary: Jo et al. develop a broadly applicable deep-learning approach to predict fluorescence (FL) based on label-free refractive index (RI) measurements, 'RI2FL' (RI to FL). The trained model can be used across cell types without retraining.
NATURE CELL BIOLOGY
(2021)
Article
Chemistry, Physical
Anisha Anand, Hong-Jyuan Jian, Hao-Hsin Huang, Li Er Hean, Yu-Jia Li, Jui-Yang Lai, Hung-Da Chou, Yu-Chuan Kang, Wei-Chi Wu, Chi-Chun Lai, Chih-Ching Huang, Huan-Tsung Chang
Summary: In this study, anti-angiogenic carbon nanovesicles (CNVs) were synthesized for sustained release of anti-VEGF antibody to treat ocular neovascularization. The CNVs showed good loading efficiency, low cytotoxicity, and strong inhibitory effects on VEGF-induced cell proliferation and migration. In vivo studies demonstrated the superior efficacy of CNVs compared to bevacizumab alone, making them a promising therapeutic agent for age-related macular degeneration.
Article
Chemistry, Physical
Shih-Chun Wei, Amit Nain, Yu-Feng Lin, Ren-Siang Wu, Pavitra Srivastava, Lung Chang, Yu-Fen Huang, Huan-Tsung Chang, Kuan-Ting Chuang, Chih-Ching Huang
Summary: Bacterial infection in chronic wounds can be effectively treated using light-triggered carbon dot liposomes (CDsomes) to accelerate the healing process by targeting the inflammation and proliferation phase.
Article
Engineering, Environmental
Hung-Yun Lin, Ka-Long Luo, Ju-Yi Mao, Chin-Jung Lin, Chen-Yow Wang, Lauren Panny, Shiow-Yi Chen, Shih-Chao Lin, Chih-Ching Huang, Scott G. Harroun, Robert Y. L. Wang, Chang-Jer Wu
Summary: Pandemic of H1N1 infections are frequently reported around the world, and the emergence of drug-resistant strains and limitations of current anti-flu drugs have increased the demand for novel antivirals. In this study, a potent alginate-derived anti-flu material, Alg@AS CNGs, was developed from seaweeds. The Alg@AS CNGs exhibited high biocompatibility and remarkable viral suppression properties against H1N1, surpassing other sulfated polysaccharides. It was found that Alg@AS CNGs could inhibit H1N1 infection by binding to viral particles and lowering oxidative stress in cells.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Duc Dung Nguyen, Chun-Hsu Yao, Shingjiang Jessie Lue, Chia-Jung Yang, Yun-Han Su, Chih-Ching Huang, Jui-Yang Lai
Summary: The study focuses on the development of topical formulations using chitosan coatings to enhance the penetration of ceria nanocapsules and achieve a controlled release of pilocarpine. The results show promising potential for the efficient management of acute glaucoma and other acute diseases.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Naveed Ahmad, Chung-Feng Jeffrey Kuo, Mujahid Mustaqeem, Arumugam Sangili, Chih-Ching Huang, Huan-Tsung Chang
Summary: In order to address the presence of fluoroquinolone antibiotics in aquatic environments, it is necessary to design a semiconductor photocatalyst with excellent photocatalytic efficiency. This study explored the performance of MnNb2O6/g-C3N4 composites in the photocatalytic degradation of ciprofloxacin and tetracycline-HCl antibiotics. The enhanced photocatalytic activity of MNO/g-CN was found to be due to the electron's shifting between the MNO and g-CN sheets, promoting the formation of photo-generated e(-)/h(+) pairs, indicating the existence of a low-waste, high-performance material for eradicating ciprofloxacin and tetracycline-HCl from wastewater.
Article
Nanoscience & Nanotechnology
Hong-Jyuan Jian, Anisha Anand, Jui-Yang Lai, Binesh Unnikrishnan, Huan-Tsung Chang, Scott G. Harroun, Chih-Ching Huang
Summary: Effective treatment of infectious keratitis requires the elimination of pathogens, reduction of inflammation, and prevention of corneal damage. A nanocomposite (Arg-CQDs/pCur) consisting of arginine-derived carbon quantum dots (Arg-CQDs) and polymeric curcumin (pCur) was prepared. The Arg-CQDs/pCur nanocomposite showed combined antibacterial, antioxidative, anti-inflammatory, and pro-proliferative properties, making it an effective treatment for bacterial keratitis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Environmental
Wei -Yi Chu, Yi-Ru Chiou, Ren-Hao Luo, Tzu-Heng Chen, Cheng-Ju Yu, Yi-Ju Chou, Huan-Tsung Chang, Chien-Fu Chen
Summary: This article introduces a new technique for the synthesis of colloidosomes using a microfluidic droplet device and partially miscible liquids. By dissolving liquids within droplets, hydrophilic nanoparticles can be close-packed at the liquid interface to form colloidosomes. This method also enables colloidosomes to be synthesized within a narrow range of sizes. The close-packing of nanoparticles is confirmed by modifying colloidosomes into signal-enhanced lateral flow assays, which demonstrates their potential application in bacterial detection platforms, drug encapsulation, and biosensing.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Physical
Cheng-Ruei Yang, Yu-Syuan Lin, Ren-Siang Wu, Chin-Jung Lin, Han-Wei Chu, Chih-Ching Huang, Anisha Anand, Binesh Unnikrishnan, Huan-Tsung Chang
Summary: A ratiometric fluorescent probe based on o-phenylenediamine-derived carbonized polymer dots (o-PD CPDs) was developed for real-time intracellular detection of singlet oxygen (1O2). The o-PD CPDs can produce 1O2 and selectively quench the yellow fluorescence, allowing for the selective detection of 1O2 against other reactive oxygen species (ROS). The probe was successfully applied to monitor 1O2 level in the intracellular environment and detect 1O2 and other ROS species in an inflammatory neutrophil cell model.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Girum Getachew, Chien-Hua Hsiao, Aswandi Wibrianto, Akash S. Rasal, Worku Batu Dirersa, Chih-Ching Huang, Neralla Vijayakameswara Rao, Je-Hsin Chen, Jia-Yaw Chang
Summary: The design of therapeutic nanoplatforms based on fluorescent carbon dots (CDs) has become a viable strategy due to their aqueous solubility, biocompatibility, and ease of functionalization. Through doping heteroatoms into CDs structures, N-, Cl-, and S-doped CDs (NClS/CDs) and Se-, N-, and Cl-doped CDs (NClSe/CDs) were synthesized with superior optoelectronic properties. These doped CDs showed enhanced quantum efficiencies and were used for photodynamic therapy (PDT) by efficiently producing reactive oxygen species (ROS) under laser irradiation. Nanovesicles (NVs) loaded with camptothecin (CPT) drug were designed using NClS/CDs, which exhibited ROS-triggered drug release. The study presented an image-guided PDT and chemotherapeutic platform based on the NVs, showing potential for future cancer treatment.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Multidisciplinary Sciences
Snigdha Roy Barman, Shuen-Wen Chan, Fu-Cheng Kao, Hsuan-Yu Ho, Imran Khan, Arnab Pal, Chih-Ching Huang, Zong-Hong Lin
Summary: A next generation of wearable self-powered wound dressing is developed, which can be activated by diverse stimuli from the patient's body and provide on-demand treatment for both normal and infected wounds. The dressing utilizes thermocatalytic bismuth telluride nanoplates (Bi2Te3 NPs) functionalized onto carbon fiber fabric electrodes, generating hydrogen peroxide to effectively inhibit bacterial growth at the wound site. The integrated electrodes connected to a wearable triboelectric nanogenerator (TENG) accelerate wound closure by enhancing cellular proliferation, migration, and angiogenesis. This self-powered dressing has great potential in personalized and user-friendly wound care with improved healing outcomes.
Article
Chemistry, Physical
Amit Nain, Yu-Ting Tseng, Akash Gupta, Yu-Feng Lin, Sangili Arumugam, Yu-Fen Huang, Chih-Ching Huang, Huan-Tsung Chang
Summary: We have developed multifunctional nanogels with antimicrobial, antioxidant, and anti-inflammatory properties, facilitating rapid wound healing. The nanogels, formed using quercetin and a carbonization process, possess excellent antioxidative and bacterial targeting properties. By incorporating copper sulfide nanoclusters, the nanogels show enhanced antimicrobial effects and can eradicate biofilms in diabetic mice wounds. The presence of the nanogels also regulates the expression of inflammatory and anti-inflammatory factors, promoting angiogenesis, epithelialization, and collagen synthesis for accelerated wound healing.
NANOSCALE HORIZONS
(2023)