☆ 4.6 Article

Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

ANALYST (2010)

期刊

ANALYST

卷 135, 期 6, 页码 1370-1374

出版社

ROYAL SOC CHEMISTRY

DOI: 10.1039/b927012d

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类别

Chemistry, Analytical

资金

BMBF [05KS7PC2]
DFG [HA2394/12-2]

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摘要

Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus, the neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of ultraviolet radiation on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milliseconds. Chemical changes within the sub-cellular structure of the sperm cells were recorded as a function of ultraviolet light exposure time, showing the proof-of-principle that Raman microspectroscopy can be a fast diagnostic method for detecting the mitochondrial and motility status of human spermatozoa.

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