期刊
ANALYST
卷 135, 期 3, 页码 545-549出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b924014d
关键词
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资金
- National Basic Research Program of China [2006CB705700]
- National Natural Science Foundation of China [20975055]
- Natural Science Foundation of Tianjin [08JCZDJC21200]
A highly sensitive and selective Hg2+ detection method was developed based on the Hg2+-mediated formation of split G-quadruplex-hemin DNAzymes. In this method, two label-free oligonucleotides are used. In the presence of Hg2+, the two oligonucleotides hybridize to each other to form a duplex, in which T-T mismatches are stabilized by T-Hg2+-T base pair. As a result, the G-rich sequences of the two oligonucleotides can associate to form a split G-quadruplex, which is able to bind hemin to form the catalytically active G-quadruplex-hemin DNAzymes. This can be reflected by an absorbance increase when monitored in the H2O2-ABTS (2,2'-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid) reaction system by using UV-vis absorption spectroscopy. This 'turn-on' process allows the detection of aqueous Hg2+ at concentrations as low as 19 nM using a simple colorimetric technique. With the development of the studies on metal-base pairs, this Hg2+-sensing method can be easily extended to the analysis of other metal ions.
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