Article
Chemistry, Analytical
Tanja Ducic, Milena Ninkovic, Immaculada Martinez-Rovira, Swetlana Sperling, Veit Rohde, Dragoljub Dimitrijevi, Gabriel Vicent Jover Manas, Lisa Vaccari, Giovanni Birarda, Ibraheem Yousef
Summary: This study focused on the biochemical profiling of live GBM cells to understand tumor heterogeneity. Riluzole treatment affected carbohydrate metabolism and DNA structure in all GBM cell lines. There were also changes in lipid structures and protein secondary conformation after riluzole treatment.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yadi Wang, Wentao Dai, Zhixiao Liu, Jixiang Liu, Jie Cheng, Yuanyuan Li, Xueling Li, Jun Hu, Junhong Lu
Summary: A method based on single-cell FTIR microspectroscopy was developed to quantitatively evaluate cellular heterogeneity by calculating the cell-to-cell similarity distance, revealing that infrared phenotypes may reflect dynamic heterogeneity changes in cell populations. This provides an alternative label-free optical approach for quantifying cellular heterogeneity and can be combined with other single-cell analysis tools to understand the genotype-to-phenotype relationship in cellular populations.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jakub Maciej Surmacki, Isabel Quiros-Gonzalez, Sarah Elizabeth Bohndiek
Summary: This study demonstrates the capability of Raman spectroscopy in monitoring redox biochemical changes in cells under different antioxidant and pro-oxidant environments. The results show that Raman spectroscopy can accurately characterize the intracellular conditions associated with oxidative stress, providing valuable insights for further research in this area.
Article
Chemistry, Analytical
Alice Spadea, Joanna Denbigh, M. Jayne Lawrence, Mustafa Kansiz, Peter Gardner
Summary: This study demonstrates the first use of a novel completely optically-based photothermal method to obtain infrared spectra of biological cells and to combine it with Raman spectra at submicron spatial resolution. The new instrumentation is capable of analyzing subcellular structures, identifying protein secondary structures, and lipid-rich bodies on the submicron scale, showing great potential in cell analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Lewis M. Dowling, Paul Roach, Eirik A. Magnussen, Achim Kohler, Srinivas Pillai, Daniel G. van Pittius, Ibraheem Yousef, Josep Sule-Suso
Summary: Liquid biopsies have the potential to improve personalized cancer management, but current methods for identifying circulating tumor cells are not reliable enough. Fourier Transform Infrared (FTIR) microspectroscopy could be a new technique for identifying these cells, regardless of tumor type.
Review
Instruments & Instrumentation
Ahmed Refaat, Gihan Kamel
Summary: This article introduces the important role of synchrotron radiation-FTIR microspectroscopy in the field of biomedical research, with a focus on neurodegenerative diseases, diabetes, preeclampsia, and other relevant areas.
APPLIED SPECTROSCOPY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Ines Delfino, Valerio Ricciardi, Maria Lepore
Summary: Fourier transform infrared microspectroscopy using a synchrotron radiation source has great potential in studying the ionizing radiation effects on human cells. It has been successfully employed in shedding light on cell processes exposed to hadron therapy, and can contribute to the improvement of cancer treatment strategies.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Applied
Xiao-Bo Xu, Yan-Yang He, Fenglin Wang, Wen Chen, Li Juan Tang, Jian-Hui Jiang
Summary: Researchers have developed a near-infrared fluorescent indicator called TAQ, which allows for activatable imaging of G4 structures in live cells. The indicator interacts with different G4 structures and exhibits high selectivity for G4s over other nucleic acids. It has a wide dynamic range for detecting G4 concentrations and can be used to track the dynamics of RNA/DNA G4s in live cells.
Article
Environmental Sciences
Jeff Wagner, William Robberson, Harry Allen
Summary: This study demonstrates how the precision of microplastic (MP) analyses can be assessed in a laboratory setting. The results show that the MP methods used in different laboratories generally yield repeatable results. The precision estimates obtained from the analyses can be used to ensure the quality of MP results and compare them to other data. Additionally, re-analyses of reference samples can be used to assess and compare precision between laboratories.
Article
Chemistry, Multidisciplinary
Hye-Youn Jung, Boram Kim, Min Ho Jeon, Yoonkyung Kim
Summary: Fluorescence imaging is an indispensable tool in modern biological research, but it is limited by autofluorescence, light scattering, and limited penetration depth of light in living systems. This study prepares a water-soluble and safe fluorescence photoswitch for near-infrared imaging in living systems.
Article
Environmental Sciences
Josef Brandt, Franziska Fischer, Elisavet Kanaki, Kristina Enders, Matthias Labrenz, Dieter Fischer
Summary: Analyzing the environmental occurrence of MP particles has received significant attention in the past decade, with a focus on effective risk assessment through accurate sampling and analysis methods. The choice of subsampling methods is crucial for reducing MP particle count errors, and increasing the fraction of MP particles in samples can help achieve lower errors.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2021)
Article
Spectroscopy
Xiaojie Zhou, Weilin Wang, Jiajia Zhong, Wenjie Yu, Yuzhao Tang, Qun Shu
Summary: Gestational diabetes mellitus (GDM) is a common complication during pregnancy that can have severe effects on the health of the mother and newborn. This study used Fourier transform infrared microspectroscopy to analyze the biochemical composition of GDM placental tissue and found changes in lipid conformation, increased lipid oxidation, and alterations in protein structure. These findings provide insight into how GDM affects the placenta and causes metabolic changes in the mother and fetus.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Agricultural Engineering
Johnny Beaugrand, Camille Goudenhooft, Camille Alvarado, Marie-Francoise Devaux, Camille Rivard, Sylvie Durand, Hugo Chauvet, Matthieu Refregiers, Frederic Jamme, Fabienne Guillon, Christophe Baley, Alain Bourmaud
Summary: The study focused on the cell wall composition dynamics of flax stems, investigating the fluorescence emission patterns and intensity differences after a 90 degrees tilt. Results showed a significant increase in fluorescence intensity in tilted plants, and differences in stem fluorescence intensity were observed among different developmental stages.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Analytical
Hanchuang Zhu, Xue Zhang, Caiyun Liu, Yan Zhang, Meijun Su, Xiaodi Rong, Xin Wang, Mengyuan Liu, Xiaohui Zhang, Wenlong Sheng, Baocun Zhu
Summary: This study developed a fluorescent probe CB-NIR for the real-time reversible detection of volatile SO2 and FA, with many optical advantages.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Environmental
Shumin Huang, Xiaoxin Huang, Ran Bi, Qiuxia Guo, Xiaolin Yu, Qinghui Zeng, Ziyu Huang, Tianming Liu, Haisheng Wu, Yuliang Chen, Jialong Xu, Yinge Wu, Pi Guo
Summary: Microplastic pollution is an emerging environmental problem that hasn't received much research attention regarding its impact on human health. This study investigated the presence of microplastics in human sputum and their potential inhalation by analyzing sputum samples from 22 patients with different respiratory diseases. The results showed that microplastics are present in all sputum samples, suggesting inhalation as a potential pathway for plastic entry into the human body.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
