Article
Optics
Rongji Li, Angze Li, Yunhao Su, Lirong Qiu, Weiqian Zhao
Summary: Non-axial scanning confocal Raman microscopy (NASCRM) is proposed to achieve high-precision topography imaging and Raman mapping by constructing an axial focusing system with a large range and high precision. Compared with CRM using confocal microscopy (CM) focusing, NASCRM improves the axial focusing resolution by 5.3 times and the Raman mapping speed by 2.9 times. This study provides a novel method for both conventional CRM and CRM-based imaging in various fields.
OPTICS AND LASERS IN ENGINEERING
(2023)
Article
Chemistry, Analytical
Kunhua Zhou, Zhizheng Wu, Tianyu Zhang, Feng Li, Azhar Iqbal, Suresh Sivanandam
Summary: A laser scanning confocal microscope (LSCM) is widely used in the field of biological detection to study sub-micron structures. However, the optical aberrations introduced by living biological tissue limit the illumination depth of LSCMs and affect the image resolution. In this study, an image-based adaptive optics technology with an optimized stochastic parallel gradient descent (SPGD) algorithm and adaptive coefficient is proposed to minimize the effect of optical aberrations. Experimental results show that this adaptive optics system effectively reduces aberration interference during depth imaging.
Review
Optics
Jiao Bai, Xinghui Li, Xiaohao Wang, Jianjian Wang, Kai Ni, Qian Zhou
Summary: Dispersion is a common phenomenon in broadband optical systems that affects displacement measurements on sample surfaces. A novel strategy using self-reference spectrum corrects the dispersion from unstable light sources or alterable sample surfaces, making the focus-wavelength unaffected. Robustness tests demonstrate that the self-reference dispersion correction significantly improves measurement accuracy against different light sources and sample surfaces.
OPTICS AND LASERS IN ENGINEERING
(2021)
Article
Engineering, Multidisciplinary
Chunmin Liu, Guoyi Lu, Chunyang Liu, Dong Li
Summary: Based on the high measurement accuracy, fast response speed, and good stability, we propose an integrated compact chromatic confocal sensing system that overcomes device-integrating constraints in industrial environments. We introduce a spectral characteristic compensation algorithm and a peak wavelength extraction method based on Gaussian curve fitting to accurately extract the peak wavelength. A segmented curve calibration algorithm is then applied to achieve accurate mapping between peak wavelength and position. Additionally, a simple thickness measurement model and calibration procedure that do not require previous parameters are proposed for transparent objects. Experimental results demonstrate the effectiveness and feasibility of the proposed sensing system, with a root mean square error (RMSE) of less than 0.1 μm for displacement measurement and less than 1 μm for thickness measurement.
MEASUREMENT SCIENCE AND TECHNOLOGY
(2023)
Article
Optics
Qing Yu, Chong Wang, Yali Zhang, Shengming Hu, Ting Liu, Fang Cheng, Yin Wang, Tianliang Lin, Lin Xi
Summary: In this paper, an error correction method for thickness measurement of transparent samples is proposed. By analyzing and simulating the error correction model, the requirement of measurement at different axial positions is achieved.
Article
Mechanics
Arianna Berto, Pascal Lavieille, Marco Azzolin, Stefano Bortolin, Marc Miscevic, Davide Del Col
Summary: This study investigated the liquid film thickness and heat transfer coefficients during annular flow condensation in a small diameter channel, using specialized measurement techniques and algorithms to analyze the wave structures of the liquid film. The experimental results contribute to a better understanding of the heat transfer mechanisms involved in phase change processes.
INTERNATIONAL JOURNAL OF MULTIPHASE FLOW
(2021)
Article
Anatomy & Morphology
Marcus N. Leiwe, Satoshi Fujimoto, Takeshi Imai
Summary: In this study, chromatic aberrations in multicolor imaging of large samples were evaluated and found to be particularly problematic in axial aberrations, often dependent on depth. A program was developed to align depths for different fluorescence channels based on reference samples, which successfully corrected chromatic aberrations found in confocal images of multicolor-labeled brain tissues. The simple post hoc correction strategy proved useful for obtaining large-scale multicolor images of cleared tissues with minimal chromatic aberrations.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Chemistry, Analytical
Mei Li, Lu Zhang, Ling-Li Jiang, Zhi-Bo Zhao, You-Hua Long, Dong-Mei Chen, Jun Bin, Chao Kang, Ya-Juan Liu
Summary: This study proposed a label-free, high-throughput imaging method for cellular investigation of apple fruit ring rot infected by Botryosphaeria dothidea. By using confocal Raman microspectroscopic imaging technology combined with multivariate curve resolution-alternating least squares algorithm (MCR-ALS), accurate and pure molecular imaging was achieved. Additionally, a rapid and non-destructive classification method was developed using a fiber-optic probe near-infrared reflection spectrometer in conjunction with machine learning.
Article
Microbiology
Wanying He, Ruining Cai, Shichuan Xi, Ziyu Yin, Zengfeng Du, Zhendong Luan, Chaomin Sun, Xin Zhang
Summary: Microorganisms play a significant role in the formation of deep-sea elemental sulfur, and understanding their growth and dynamic sulfur metabolism is crucial for studying the deep-sea sulfur cycle. However, existing methods have limitations in conducting near real-time nondestructive metabolic studies of microorganisms.
MICROBIOLOGY SPECTRUM
(2023)
Article
Optics
Bai Haodong, Xu Nan, Zhou Jiankang, Zou Kuaisheng, Shen Weimin
Summary: This study aims to design a long-axial dispersive spectral confocal lens with chromatic dispersion to enhance the measurement accuracy and range. The design reduces the axial length of the lens by about 35% compared with conventional finite-range lenses. Increasing the image numerical aperture improves the signal-to-noise ratio but affects linearity and dispersion range. The design of a compact and efficient dispersion lens is crucial for miniaturized measuring instruments.
ACTA OPTICA SINICA
(2023)
Article
Optics
Siyi Qiu, Haihua Fan, Lin He
Summary: Microwave radiation has been found to have effects on dry spores at the molecular level, as well as on their germination, growth, and morphology. The microwave treatment leads to an increase in Ca2+-dipicolinic acid content and denaturation of nucleic acid in spores. Additionally, it inhibits the germination and growth rates of spores, causing them to become wrinkled and rougher.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2023)
Article
Engineering, Multidisciplinary
Alexander Gyurkovich, Carsten Mehring
Summary: The chromatic confocal measurement technique is used to study the dynamics of liquid sheets generated by liquid jet impinging on a harmonically excited cylinder. Comparison is made between measurements of sinuous-mode capillary waves and experimental results reported by Bremond et al. (2007) as well as analytical solutions from two inviscid linear theories. The agreement between experimental observations and thinning theory is found in terms of radial positions, but the absolute amplitude values are significantly overpredicted.
ATOMIZATION AND SPRAYS
(2023)
Article
Optics
Bai Haodong, Zhao Yang, Zhou Jiankang, Zhu Jiacheng, Shen Weimin
Summary: This paper proposes a design method for a wide-field dispersion lens, which can be used for various applications by adjusting its performance parameters. By replacing the back group, the lens's performance parameters can be adjusted to meet the needs of different measurement objects. Experimental tests demonstrate the feasibility of the proposed design method.
ACTA OPTICA SINICA
(2023)
Article
Spectroscopy
Shenghu Li, I-Ming Chou
Summary: In this study, the alpha-beta quartz phase transition temperatures were determined using in situ Raman spectroscopy at different pressures. The results showed that the Raman peak near 128 cm(-1) is more sensitive for detecting the phase transition. The corresponding phase transition pressure was calculated using three equations of state. The experimental results agree with previous data and can be used as a reliable pressure calibrant in HDAC.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Proceedings Paper
Engineering, Manufacturing
Harald Ries, Ralf Leutz
Summary: Chromatic aberrations are a limitation to lens imaging performance, and traditional correction methods add volume, surfaces, and cost to optical systems. A novel approach using diffractive optical elements as thin layers on glass substrates has been proposed to improve efficiency.
EIGHTH EUROPEAN SEMINAR ON PRECISION OPTICS MANUFACTURING
(2021)
Editorial Material
Astronomy & Astrophysics
Jean-Pierre de Vera, Mashal Alawi, Theresa Backhaus, Mickael Baque, Daniela Billi, Ute Boettger, Thomas Berger, Maria Bohmeier, Charles Cockell, Rene Demets, Rosa de la Torre Noetzel, Howell Edwards, Andreas Elsaesser, Claudia Fagliarone, Annelie Fiedler, Bernard Foing, Frederic Foucher, Joerg Fritz, Franziska Hanke, Thomas Herzog, Gerda Horneck, Heinz-Wilhelm Huebers, Bjoern Huwe, Jasmin Joshi, Natalia Kozyrovska, Martha Kruchten, Peter Lasch, Natuschka Lee, Stefan Leuko, Thomas Leya, Andreas Lorek, Jesus Martinez-Frias, Joachim Meessen, Sophie Moritz, Ralf Moeller, Karen Olsson-Francis, Silvano Onofri, Sieglinde Ott, Claudia Pacelli, Olga Podolich, Elke Rabbow, Guenther Reitz, Petra Rettberg, Oleg Reva, Lynn Rothschild, Leo Garcia Sancho, Dirk Schulze-Makuch, Laura Selbmann, Paloma Serrano, Ulrich Szewzyk, Cyprien Verseux, Jennifer Wadsworth, Dirk Wagner, Frances Westall, David Wolter, Laura Zucconi
Article
Biochemical Research Methods
Joerg Doellinger, Andy Schneider, Marcell Hoeller, Peter Lasch
MOLECULAR & CELLULAR PROTEOMICS
(2020)
Article
Microbiology
Pascal Muelner, Elisa Schwarz, Kristin Dietel, Helmut Junge, Stefanie Herfort, Max Weydmann, Peter Lasch, Tomislav Cernava, Gabriele Berg, Joachim Vater
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Chemistry, Analytical
Joerg Doellinger, Christian Blumenscheit, Andy Schneider, Peter Lasch
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Peter Lasch, Andy Schneider, Christian Blumenscheit, Joerg Doellinger
MOLECULAR & CELLULAR PROTEOMICS
(2020)
Article
Microbiology
Joerg Doellinger, Andy Schneider, Timo D. Stark, Monika Ehling-Schulz, Peter Lasch
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Chemistry, Analytical
Christian Blumenscheit, Yvonne Pfeifer, Guido Werner, Charlyn John, Andy Schneider, Peter Lasch, Joerg Doellinger
Summary: This study introduces an unbiased proteomics workflow for rapid detection of bacterial species and AMR-related proteins. Validation showed high sensitivity and specificity, making it a promising tool for clinical microbiology.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Pascal Muelner, Elisa Schwarz, Kristin Dietel, Stefanie Herfort, Jennifer Jaehne, Peter Lasch, Tomislav Cernava, Gabriele Berg, Joachim Vater
Summary: Paenibacilli are efficient producers of potent agents against bacterial and fungal pathogens, producing lipopeptides like fusaricidins and polymyxins. These compounds are of interest in medicine and agrobiotechnology for their potential therapeutic applications, particularly against multiresistant Gram-negative bacteria. Volatile organic compounds (VOCs) production is strain and medium-dependent, with Paenibacilli known for forming volatile pyrazine derivatives.
Article
Multidisciplinary Sciences
Christian Blumenscheit, Jennifer Jaehne, Andy Schneider, Jochen Blom, Thomas Schweder, Peter Lasch, Rainer Borriss
Summary: Here, we report the complete genome sequence data of the biocontrol strains Bacillus velezensis BP1.2A and BT2.4 isolated from Vietnamese crop plants, which are closely related to the model strain for plant growth promoting bacilli, FZB42.
Article
Microbiology
Jacob Heinz, Joerg Doellinger, Deborah Maus, Andy Schneider, Peter Lasch, Hans-Peter Grossart, Dirk Schulze-Makuch
Summary: Little is known about the toxicity of perchlorates for microorganisms on the cellular level. This study conducted the first proteomic investigation on the perchlorate-specific stress responses of the halotolerant yeast Debaryomyces hansenii and compared them to known salt stress adaptations. The study found that the responses to NaCl and NaClO4-induced stresses share many common metabolic features, but also identified several new perchlorate-specific stress responses. These findings are relevant for potential life on Mars.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mickael Baque, Theresa Backhaus, Joachim Meessen, Franziska Hanke, Ute Boettger, Nisha Ramkissoon, Karen Olsson-Francis, Michael Baumgartner, Daniela Billi, Alessia Cassaro, Rosa de la Torre Noetzel, Rene Demets, Howell Edwards, Pascale Ehrenfreund, Andreas Elsaesser, Bernard Foing, Frederic Foucher, Bjorn Huwe, Jasmin Joshi, Natalia Kozyrovska, Peter Lasch, Natuschka Lee, Stefan Leuko, Silvano Onofri, Sieglinde Ott, Claudia Pacelli, Elke Rabbow, Lynn Rothschild, Dirk Schulze-Makuch, Laura Selbmann, Paloma Serrano, Ulrich Szewzyk, Cyprien Verseux, Dirk Wagner, Frances Westall, Laura Zucconi, Jean-Pierre P. de Vera
Summary: Two rover missions to Mars aim to detect biomolecules as evidence of past or present life. However, the stability of Raman-detectable biomolecules in the martian environment is still uncertain. To investigate this, seven biomolecules were exposed to a simulated martian environment for 469 days. Ultraviolet radiation had a significant impact on Raman spectra, but little change was observed when samples were shielded from UV. These findings support Mars missions searching for biosignatures underground and lay the foundation for a consolidated space-proven database of spectroscopy biosignatures in targeted environments.
Article
Microbiology
Jennifer Jaehne, Thanh Tam Le Thi, Christian Blumenscheit, Andy Schneider, Thi Luong Pham, Phuong Thao Le Thi, Jochen Blom, Joachim Vater, Thomas Schweder, Peter Lasch, Rainer Borriss
Summary: This study presents the draft genome sequences of 59 endospore-forming Gram-positive bacterial strains isolated from Vietnamese crop plants. Several of these strains represent novel genomospecies and showed the potential for promoting plant growth and biocontrol against phytopathogens. Genome mining also identified 36 novel natural product biosynthesis gene clusters.
Article
Biochemical Research Methods
Joerg Doellinger, Christian Blumenscheit, Andy Schneider, Peter Lasch
Summary: Reducing the number of data points per peak in short-gradient DIA significantly increases protein identifications while maintaining quantitative precision.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
Cecilia Beatriz Figoli, Marcelo Garcea, Claudio Bisioli, Valeria Tafintseva, Volha Shapaval, Mariana Gomez Pena, Luz Gibbons, Fernando Althabe, Osvaldo Miguel Yantorno, Marcos Horton, Jurgen Schmitt, Peter Lasch, Achim Kohler, Alejandra Bosch
Summary: The study established a metabolomic-based approach using FTIR spectroscopy to identify the most competent embryos for transfer. Analysis revealed a highly heterogeneous nature of nonimplanted embryos' supernatant spectra, with approximately 60% exhibiting diverse metabolic fingerprints.
Article
Microbiology
Le Thi Thanh Tam, Jennifer Jaehne, Pham Thi Luong, Le Thi Phuong Thao, Le Thi Kim Chung, Andy Schneider, Christian Blumenscheit, Peter Lasch, Thomas Schweder, Rainer Borriss
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)