Article
Chemistry, Analytical
Daniel D. Vallejo, Jukyung Kang, Jill Coghlan, Carolina Rojas Ramirez, Daniel A. Polasky, Ruwan T. Kurulugama, John C. Fjeldsted, Anna A. Schwendeman, Brandon T. Ruotolo
Summary: The study demonstrates the sensitivity of CIU to changes in mAb structure induced by heat stress, with DSC being less sensitive to these alterations. Both instrumental platforms are able to differentiate mAbs, although minor differences between the results obtained on the two instruments were observed. CIU-based heatmaps and classification offer a rapid assessment of the most differentiating charge states for analysis of infliximab, conservatively estimated to improve mAb stability and HOS measurements' efficiency by 30-fold compared to standard DSC tools.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yi Yang, Fan Zhang, Yutian Gan, Hui-Min Zhang, Peilu Liu, Anna Mah, Lynn Gennaro, Christian Schoneich
Summary: Characterization of antibody charge heterogeneity is crucial in antibody drug development. This study presents a new workflow combining untargeted and targeted analyses to identify and characterize the induced acidic variants in a highly oxidized IgG1 antibody. The study identified 28 site-specific oxidation products as the sources of the induced charge heterogeneity and provides new insights into understanding the acidic charge heterogeneity of antibody drugs. The characterization workflow presented in this study can be widely applied as a platform approach in the biotechnology industry for in-depth characterization of antibody charge variants.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi Yang, Fan Zhang, Yutian Gan, Hui-Min Zhang, Peilu Liu, Anna Mah, Lynn Gennaro, Christian Schoeneich
Summary: Characterization of antibody charge heterogeneity is crucial for antibody drug development. A correlation between acidic charge heterogeneity and metal catalyzed oxidation has been observed, but the acidic variants induced by metal-catalyzed oxidation are not fully understood. This study presents a new characterization workflow combining untargeted and targeted analyses to identify and characterize acidic variants in a highly oxidized IgG1 antibody. The results provide new insights into the acidic charge heterogeneity of antibody drugs and offer a platform for in-depth characterization of antibody charge variants.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Kiran Patil, Amin Pathan, Sukanta Naik, Vineet Zope, Rajesh Chavan, Ravindra Yeole
Summary: A precise and accurate LC-MS/MS bioanalytical method has been developed and validated for quantification of WCK 4234 and meropenem in dog plasma, allowing assessment of pharmacokinetics in beagle dogs.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Chemistry, Analytical
Deseree J. Reid, Tapasyatanu Dash, Zhihan Wang, Craig A. Aspinwall, Michael T. Marty
Summary: Daptomycin is a cyclic lipopeptide antibiotic that interacts with lipid membranes in a non-specific manner. The interaction strength depends on membrane rigidity, with more rigid membranes showing stronger interactions. More fluid membranes may undergo pore formation to expose daptomycin to oxidation. These findings highlight the complementary use of native mass spectrometry, fast photochemical oxidation of peptides, and membrane conductance experiments in studying antibiotic-membrane interactions.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yue Huang, Si Mou, Yadi Wang, Ruipeng Mu, Meina Liang, Anton Rosenbaum
Summary: Antibody-drug conjugates (ADCs) present challenges in bioanalysis due to their intricate structures and complex metabolism. Traditional measurement methods involve quantifying ADCs and Total Ab, but recent advances in instrumentation, such as high resolution mass spectrometers (HRMS), allow for more precise analysis of large molecules like ADCs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Juraj Konc, Libby Brown, Daniel R. Whiten, Yukun Zuo, Peter Ravn, David Klenerman, Goncalo J. L. Bernardes
Summary: A new bioconjugation platform has been developed for the preparation of DNA-antibody bioconjugates using a simple reagent, resulting in improved homogeneity of the prepared conjugates suitable for fluorescence or super-resolution microscopy cell imaging experiments.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Juan L. Benede, Alberto Chisvert, Rafael Lucena, Soledad Cardenas
Summary: The study presents a proof-of-concept of using a polyamide-coated paper-based sorptive phase for simultaneous extraction of six different penicillin-derived antibiotics from human saliva samples. The method features simple synthesis, low cost, and high efficiency.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Carolina Rojas Ramirez, Jessica A. Espino, Lisa M. Jones, Daniel A. Polasky, Alexey I. Nesvizhskii
Summary: Monitoring protein structure before and after environmental alterations can provide insights into protein function. Fast photochemical oxidation of proteins (FPOP) coupled with mass spectrometry allows for monitoring of structural rearrangements by exposing proteins to radicals. However, the challenges of processing FPOP data have limited its proteome-scale uses. In this study, a computational workflow was developed to analyze FPOP data sets more efficiently, enabling the identification of modified peptide spectra at a faster rate than previous methods.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Juraj Piestansky, Michaela Matuskova, Ivana Cizmarova, Dominika Olesova, Peter Mikus
Summary: A capillary electrophoresis-mass spectrometry method was developed and validated for quantifying branched chain amino acids in various samples, showing favorable validation parameters and good agreement with declared content. This method represents a suitable alternative to traditional approaches in branched chain amino acid quality control and bioanalysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Roderick Corstiaan Abraham Versloot, Patricia Arias-Orozco, Matthijs Jonathan Tadema, Florian Leonardus Rudolfus Lucas, Xinghong Zhao, Siewert J. J. Marrink, Oscar Paul Kuipers, Giovanni Maglia
Summary: Mass spectrometry cannot distinguish between molecules with the same mass-to-charge ratio, but nanopore technology can differentiate diastereomeric and enantiomeric differences in peptides that cannot be observed by conventional MS analysis. The resolution in nanopore analysis is likely due to multiple chiral interactions during peptide transport across the nanopore, similar to chiral chromatography. Nanopore technology also offers possibilities for the rapid and cost-effective analysis of peptides, including those that cannot be easily differentiated by mass spectrometry.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemical Research Methods
Max Sauter, Kathrin Foerster, Julia Benzel, Stefan Pfister, Kristian W. Pajtler, Walter E. Haefeli, Juergen Burhenne
Summary: An UPLC-MS/MS assay was established for the investigation of selinexor in mice, with high sensitivity and scalable sample processing capabilities. The assay showed high accuracy and was suitable for studying selinexor in small animal experiments.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Cindy X. Cai, Nicole A. Schneck, Taryn Cozine, Vera B. Ivleva, Daniel Ragheb, Deepika Gollapudi, Aakash Patel, Nathan Barefoot, Daniel B. Gowetski, Q. Paula Lei
Summary: Research on rTTHC during purification process revealed the coexistence of three different forms of cysteine modification, providing crucial guidance for vaccine development.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Multidisciplinary
Corinne A. Lutomski, Tarick J. El-Baba, Joshua D. Hinkle, Idlir Liko, Jack L. Bennett, Neha V. Kalmankar, Andrew Dolan, Carla Kirschbaum, Kim Greis, Leonhard H. Urner, Parth Kapoor, Hsin-Yung Yen, Kevin Pagel, Christopher Mullen, John E. P. Syka, Carol V. Robinson
Summary: By using an infrared laser in a high-pressure linear ion trap, researchers were able to remove detergent micelles and obtain good sequence coverage for membrane proteins and their complexes through top-down mass spectrometry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Deseree J. Reid, Tapasyatanu Dash, Zhihan Wang, Craig A. Aspinwall, Michael T. Marty
Summary: In this study, native mass spectrometry (MS) and fast photochemical oxidation of peptides (FPOP) were used to investigate the interactions between the antibiotic daptomycin and different lipid bilayer nanodiscs. The results showed that daptomycin incorporates randomly into bilayers and has stronger interactions with rigid membranes. Pore formation may occur in fluid membranes, exposing daptomycin to oxidation. These findings demonstrate the complementarity of native MS, FPOP, and membrane conductance experiments in understanding antibiotic peptide-membrane interactions.
ANALYTICAL CHEMISTRY
(2023)
