Review
Genetics & Heredity
Quentin Menetrey, Pauline Sorlin, Estelle Jumas-Bilak, Raphael Chiron, Chloe Dupont, Helene Marchandin
Summary: In patients with cystic fibrosis, the lung microbiome experiences important selective pressures, leading to colonization by both common and emerging pathogens. Competitive interactions and evolutionary modifications among bacteria are major drivers of persistence in the CF lung, with emerging pathogens like Achromobacter xylosoxidans and Stenotrophomonas maltophilia becoming important members of the complex bacterial community in the CF lung.
Article
Cardiac & Cardiovascular Systems
Mark L. Metersky, Radmila Choate, Timothy R. Aksamit, Douglas Conrad, Nicole C. Lapinel, Diego J. Maselli, Pamela J. McShane
Summary: This study analyzed the prevalence of Stenotrophomonas maltophilia in patients with bronchiectasis and its association with patient characteristics and disease severity. The results suggest that patients with S. maltophilia may have worse outcomes, but further research is needed to confirm these trends.
RESPIRATORY MEDICINE
(2022)
Article
Immunology
Sz-Yun Pan, Yung-Luen Shih, Hsin-Hui Huang, Li-Hua Li, Yi-Tsung Lin, Tsuey-Ching Yang
Summary: This study aimed to elucidate the function and significance of the PacIRA system in Stenotrophomonas maltophilia, an important nosocomial pathogen, to better understand its characteristics and infection mechanisms.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Respiratory System
Charlotte Marsac, Laura Berdah, Guillaume Thouvenin, Isabelle Sermet-Gaudelus, Harriet Corvol
Summary: This study showed that Achromobacter xylosoxidans infection leads to worsening lung function and increased severity in children with cystic fibrosis. In the 2 years before and after infection, cases had more pulmonary exacerbations, hospitalizations, and antibiotic courses compared to controls.
Article
Microbiology
Melissa S. McDaniel, Natalie R. Lindgren, Caitlin E. Billiot, Kristina N. Valladares, Nicholas A. Sumpter, W. Edward Swords
Summary: Despite advances in treatment options for people with cystic fibrosis (CF), bacterial infections remain the main cause of illness and death in this patient population. This study reveals that Pseudomonas aeruginosa infection can enhance the persistence of Stenotrophomonas maltophilia by disrupting the integrity of the epithelial barrier. These findings provide insights into the molecular mechanisms governing this important interaction between multiple species and may have implications for understanding pulmonary diseases such as CF.
MICROBIOLOGY SPECTRUM
(2023)
Article
Respiratory System
Christian Ronn, Peter Kamstrup, Josefin Eklof, Louise Lindhardt Toennesen, Jonas Bredtoft Boel, Christian Ostergaard Andersen, Ram Benny Dessau, Jon Torgny Wilcke, Pradeesh Sivapalan, Charlotte Suppli Ulrik, Jens-Ulrik Staehr Jensen
Summary: This observational cohort study found that a lower respiratory tract culture positive for S. maltophilia in COPD patients was associated with significantly increased mortality and hospitalisation for exacerbation of COPD. Randomised controlled trials are suggested to explore the potential of antibiotic treatment targeting S. maltophilia.
RESPIRATORY RESEARCH
(2023)
Article
Infectious Diseases
Hsin-Hui Huang, Bo-Kuan Wu, Li-Hua Li, Yi-Tsung Lin, Tsuey-Ching Yang
Summary: The PhoPQ TCS in S. maltophilia plays a key role in regulating beta-lactam resistance by down-regulating specific genes, attenuating beta-lactamase activity, and increasing beta-lactam influx. Inactivation of PhoPQ leads to compromised beta-lactam resistance through alterations in gene expression and increased drug influx.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Megan Y. Nas, Jeffrey Gabell, Nicholas P. Cianciotto
Summary: Previously, it was found that Stenotrophomonas maltophilia possesses a T4SS that can kill other bacteria, with two effectors, RS14245 and RS14255, identified as key components in killing pathogenic Pseudomonas aeruginosa. These findings represent a significant advancement in understanding S. maltophilia, T4SS function, clinically relevant interactions, and bactericidal activities.
Article
Multidisciplinary Sciences
Jaclyn G. McCutcheon, Andrea Lin, Jonathan J. Dennis
Summary: This study characterizes the bacteriophage AXL1, which was isolated from soil and has the ability to selectively kill multiple pathogenic bacteria. It also demonstrates the presence of antibiotic resistance in the phage. Additionally, the study reveals the mechanism of AXL1 using the type IV pilus as its host receptor for infection.
SCIENTIFIC REPORTS
(2022)
Article
Ophthalmology
Chan-Ho Cho, Sang-Bumm Lee
Summary: Compared with PA keratitis, SM keratitis has slower progression and its clinical characteristics and treatment outcomes are not worse. In both groups, hypopyon is a risk factor for poor clinical outcomes.
EYE & CONTACT LENS-SCIENCE AND CLINICAL PRACTICE
(2021)
Article
Plant Sciences
Anu Singh, Sanjeet Kumar, Kanika Bansal, Prabhu B. Patil
Summary: Stenotrophomonas maltophilia is a versatile bacterium found in various environments, and recent studies have shown that it is a complex consisting of multiple hidden species. Several of these species have been identified as plant pathogens, requiring further taxonomic assessment for systematic studies and management.
Article
Hematology
Takashi Sakoh, Muneyoshi Kimura, Shinsuke Takagi, Sho Ogura, Masayo Morishima, Ryosuke Yamamuro, Kyosuke Yamaguchi, Mitsuhiro Yuasa, Daisuke Kaji, Kosei Kageyama, Yuki Taya, Aya Nishida, Kazuya Ishiwata, Hisashi Yamamoto, Go Yamamoto, Yuki Asano-Mori, Atsushi Wake, Naoyuki Uchida, Shuichi Taniguchi, Hideki Araoka
Summary: We developed a scoring system to distinguish Stenotrophomonas maltophilia (SM) bacteremia from Pseudomonas aeruginosa (PA) bacteremia using clinical indicators in order to avoid treatment delay. The study identified no evidence of PA colonization, antipseudomonal beta-lactam breakthrough bacteremia, and central venous catheter insertion as independent predictors of SM bacteremia. The scoring system showed good predictive performance in distinguishing SM bacteremia from PA bacteremia.
ANNALS OF HEMATOLOGY
(2023)
Article
Immunology
Nimet Melis Bilen, Zumrut Sahbudak Bal, Gizem Guner Ozenen, Sema Yildirim Arslan, Gulcihan Ozek, Nihal Ozdemir Karadas, Pinar Yazici, Feriha Cilli, Zafer Kurugol
Summary: This study aimed to evaluate the risk factors for infection and mortality associated with Stenotrophomonas maltophilia bloodstream infections in children and compare them with Pseudomonas aeruginosa bloodstream infections. Results showed that previous Pediatric Intensive Care Unit (PICU) admission, prior glycopeptide and carbapenem use were significantly more common in patients with S. maltophilia BSIs. C-reactive protein (CRP) levels were significantly higher in S. maltophilia BSIs. Prior carbapenem use was identified as a significant risk factor for developing S. maltophilia BSIs.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Michaela Prothiwa, Verena Filz, Sebastian Oehler, Thomas Boettcher
Summary: A novel substrate-based chemical probe was developed against the central quinolone biosynthesis enzyme HmqD in Burkholderia species, leading to the discovery of the first known HmqD inhibitors with single-digit micromolar efficacy in blocking quinolone production in Burkholderia cultures.
Article
Public, Environmental & Occupational Health
Veronica Franca Diniz Rocha, Thiago Pereira Cavalcanti, Jailton Azevedo, Helena Ferreira Leal, Giulyana Evelyn Oliveira Silva, Allan Roberto Xavier Malheiros, Ledilce Almeida Ataide, Jose Admirco Lima Filho, Antonio Raimundo Pinto Almeida, Nadia de Andrade Khouri, Mitermayer Galvao Reis, Joice Neves Reis
Summary: An outbreak of bloodstream infections caused by Stenotrophomonas maltophilia and Burkholderia cepacia occurred among dialyzed patients at a large hemodialysis center in Brazil. After collecting water samples and identifying genetic patterns, it was found that the outbreak was due to microbial biofilm contamination of the piping system. Changing the entire plumbing system successfully ended the outbreak.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Veterinary Sciences
Sofia Wareham-Mathiassen, Vera Pinto Glenting, Lene Bay, Marie Allesen-Holm, Henrik Bengtsson, Thomas Bjarnsholt
Summary: The microflora of pig neck skin is similar to that of human skin, although there are some differences at the species level. Pig neck skin can be used as an injection model, but there are certain limitations.
LABORATORY ANIMALS
(2023)
Article
Microbiology
Mona Bove, Mette Kolpen, Mads Lichtenberg, Thomas Bjarnsholt, Tom Coenye
Summary: In this study, the fitness, antimicrobial susceptibility, metabolic activity, gene expression, in vitro production of virulence factors and in vivo virulence of experimentally evolved Pseudomonas aeruginosa PAO1 were evaluated. Mutations in mexT and fusA1 were found in the evolved strains, leading to altered fitness, metabolic activity, mexE expression, and antimicrobial susceptibility. However, the in vitro production of virulence factors and virulence in vivo were unchanged compared to the wild-type strains. These evolved strains also showed reduced susceptibility to tobramycin in an in vivo mouse model. Overall, this study highlights the impact of experimental evolution on the fitness and antimicrobial susceptibility of Pseudomonas aeruginosa.
Article
Multidisciplinary Sciences
Yvonne Adams, Anne Skovsbo Clausen, Peter Ostrup Jensen, Malin Lager, Peter Wilhelmsson, Anna J. Henningson, Per-Eric Lindgren, Daniel Faurholt-Jepsen, Helene Mens, Peter Kraiczy, Kasper Norskov Kragh, Thomas Bjarnsholt, Andreas Kjaer, Anne-Mette Lebech, Anja R. Jensen
Summary: We screened a group of low-passage Borrelia burgdorferi s.l. isolates using a novel human-derived 3D blood-brain barrier (BBB)-organoid model. The results showed that human-derived BBB-organoids can be invaded by Borrelia spirochetes, leading to swelling and loss of structural integrity. The BBB-organoid model highlights the tropism between B. burgdorferi s.l. genospecies and their ability to cross the blood-brain barrier, contributing to central nervous system infection.
Article
Microbiology
Eva Armengol, Kasper Norskov Kragh, Tim Tolker-Nielsen, Josep M. Sierra, Doaa Higazy, Oana Ciofu, Miguel Vinas, Niels Hoiby
Summary: The combination of colistin and rifampicin has been found to effectively kill colistin-resistant Pseudomonas aeruginosa, suggesting it as a promising treatment for infections caused by this strain.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Francesco Ricci, Kshitij Tandon, Maria Mosshammer, Ellie H. -J. Cho, Linda L. Blackall, Michael Kuhl, Heroen Verbruggen
Summary: In this study, the researchers used chemical imaging and microbiome characterization to investigate the impact of physicochemical gradients on the coral skeleton microbiome. They found that the microbial community in the coral skeleton was influenced by the physicochemical environment, and the microbiome composition was linked to the abiotic conditions.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Dermatology
Anne -Sofie Halling, Blaine Gabriel Fritz, Trine Gerner, Maria Rasmussen Rinnov, Lene Bay, Mette Hjorslev Knudgaard, Nina Haarup Ravn, Simon Trautner, Iben Frier Ruge, Caroline Olesen, Isabel Diiaz-Pinees Cort, Lone Skov, Nikolaj Sorensen, Amalie Thorsti Moller Ronnstad, Simon F. Thomsen, Alexander Egeberg, Ivone Jakasa, Sanja Kezic, Thomas Bjarnsholt, Jacob P. Thyssen
Summary: This study examined the association between alterations in the skin microbiome and the risk of atopic dermatitis (AD) in a Danish birth cohort. While no overall association was found, the study showed that lower alpha diversity in the skin microbiome at 2 months of age was associated with an increased risk of AD, particularly in children with parents who have atopy.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Plant Sciences
Kasper Elgetti Brodersen, Michael Kuhl
Summary: In many terrestrial seeds, photosynthetic activity provides oxygen to the developing plant embryo. However, it is unclear whether seagrass seeds have similar photosynthetic capacity to alleviate intra-seed hypoxic stress. This study used various techniques to determine the oxygen microenvironment and photosynthetic activity in developing seeds and seedlings of seagrass. The findings showed that sheath-covered seeds had high oxygen concentrations in the photosynthetic active seed sheath and low oxygen concentrations at the position of the embryo. Photosynthesis in the seed sheath increased oxygen availability in central parts of the seed, enabling enhanced respiratory energy generation for biosynthetic activity.
Article
Medicine, General & Internal
Amalie Thorsti Moller Ronnstad, Lene Bay, Iben Frier Ruge, Anne-Sofie Halling, Blaine Gabriel Fritz, Ivone Jakasa, Rosalie Luiten, Sanja Kezic, Simon Francis Thomsen, Thomas Bjarnsholt, Jacob P. Thyssen
Summary: This study aims to investigate the changes in the skin microbiome, skin barrier, and immune response in patients with AD, as well as the temporal relationship between them. The study includes 81 AD patients and 41 matched controls, and collects skin biopsies, tape strips, skin and nasal swabs for analysis using RNA sequencing, multiplex immunoassays, liquid chromatography-mass spectrometry, and 16S rDNA analysis. The findings will be presented at national and international conferences and published in international peer-reviewed publications.
Article
Environmental Sciences
Swathi Murthy, Cristian Picioreanu, Michael Kuehl
Summary: Reef building corals have complex structures to maximize photosynthesis while minimizing damage and stress. A multiphysics modeling approach was used to simulate the internal environment of corals, including light, temperature, and oxygen distribution. The model results were consistent with spatial measurements, providing insights into the effect of coral morphology and light scattering on the coral's internal environment.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Infectious Diseases
Torgny Sunnerhagen, Franziska Schwartz, Lars Christophersen, Thomas Bjarnsholt, Klaus Qvortrup, Nikolaj Eldrup, Katja Vogt, Claus Moser
Summary: This study examines whether Pseudomonas aeruginosa and Staphylococcus aureus form biofilm on EVAR prostheses. The results show that bacteria can rapidly adhere to and form antibiotic-tolerant biofilms on EVAR graft material.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Microbiology
Bruno Jesus, Thierry Jauffrais, Erik Trampe, Vona Meleder, Lourenco Ribeiro, Joan M. Bernhard, Emmanuelle Geslin, Michael Kuhl
Summary: Intertidal microphytobenthic (MPB) biofilms play a key role in coastal primary production through the movement and vertical migration of diatoms exhibiting photo-regulation. This study compared the photo-regulation features and non-photochemical quenching (NPQ) responses of four epipelic diatom species from a natural MPB biofilm. The results showed species-specific photo-regulation and a wide range of NPQ, suggesting the coexistence of epipelic species in compacted light niches. Additionally, the study revealed that diatom cell orientation within MPB can be modulated by light, highlighting the importance of considering species-specific responses and understanding cell orientation and photo-behaviour in MPB research.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biology
Niclas Heidelberg Lyndby, Swathi Murthy, Sandrine Bessette, Sofie Lindegaard Jakobsen, Anders Meibom, Michael Kuhl
Summary: The study used optical coherence tomography (OCT) to investigate the morphology and tissue optical properties of Cassiopea jellyfish. The results showed that white granules in Cassiopea enhanced the local light-availability for symbionts, leading to higher carbon fixation compared to tissue without these granules.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Correction
Multidisciplinary Sciences
Rachel Alderdice, Gabriela Perna, Anny Cardenas, Benjamin C. C. Hume, Martin Wolf, Michael Kuhl, Mathieu Pernice, David J. Suggett, Christian R. Voolstra
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Anne Sofie Laulund, Franziska Angelika Schwartz, Lars Christophersen, Mette Kolpen, Peter Ostrup Jensen, Henrik Calum, Niels Hoiby, Kim Thomsen, Claus Moser
Summary: Chronic wounds have compromised microcirculation and are often infested with microorganisms in biofilms, hindering antibiotic function. This study investigates whether hyperbaric oxygen therapy (HBOT) can counteract this process.