期刊
ANAEROBE
卷 18, 期 3, 页码 344-349出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.anaerobe.2012.04.004
关键词
Microbial diversity; Fermentation; Wild rabbits; Farm rabbits; Caecum
类别
资金
- Ministerio de Educacion y Ciencia (Spain) [AGL 2006-07596]
- Universidad Nacional Experimental del Tachira (Venezuela)
- Spanish National Research Council (CSIC, JAE-Doc Programme)
In order to study the microbial caeca] ecosystem of wild and domestic rabbits through the fermentation characteristics and concentration and diversity of bacterial and archaeal communities, caecal samples from sixteen wild rabbits (WR) were contrasted with two groups (n = 4) of farm rabbits receiving low (LSF) or high (HSF) soluble fibre diets from 28 (weaning) to 51 days of age. DNA was extracted for quantifying bacteria and Archaea by qPCR and for biodiversity analysis of microbial communities by DGGE. Samples from WR had lower caecal pH and ammonia and higher volatile fatty acids concentration than farm animals. Lower acetate and higher butyrate proportions were detected in WR. Bacterial and archaeal DGGE profiles were clearly different between wild and farm rabbits, and diet-affected population of farm rabbits. Similarity index of bacteria was lower than 0.40 among WR, and 0.52 among farm rabbits. In conclusion, caecal fermentation characteristics differ between wild and farm rabbits, which harbour clearly different bacterial and archaeal communities. In farm rabbits, diversity is influenced by the dietary level of soluble fibre. (C) 2012 Elsevier Ltd. All rights reserved.
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