期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 378, 期 -, 页码 264-269出版社
ELSEVIER
DOI: 10.1016/j.ijms.2014.08.035
关键词
Exosomes; Top-down proteomics; Post-translational modifications; Cytokines; Histones
资金
- NIH [GM021248]
Top-down analysis is reported for a portion, of the protein cargo of exosomes shed by myeloid-derived suppressor cells that participate in intracellular signaling in the tumor microenvironment. Instrument mass resolution limited the study to proteins of molecular masses below 30 kDa. A two-step fractionation strategy was used, including open tubular gel electrophoresis and C3 reverse phase high performance liquid chromatography. Twenty-one unique proteins were identified among more than 200 proteoforms, and comprising primarily two functionally important protein families: the S100 proinflammatory mediators and an abundance of histones. Fifty-six percent of the total protein in these exosomes was determined to comprise histones, of which H2B variants contribute 42%. (C) 2014 Elsevier B.V. All rights reserved.
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