Article
Biochemistry & Molecular Biology
Mark Marsland, Amiee Dowdell, Chen Chen Jiang, James S. Wilmott, Richard A. Scolyer, Xu Dong Zhang, Hubert Hondermarck, Sam Faulkner
Summary: There is increasing evidence suggesting the involvement of nerve growth factor (NGF) and its receptors in cancer growth. In this study, the expression of NGF, proNGF, TrkA, p75(NTR) and sortilin in melanoma was investigated. The results showed downregulation of NGF/ProNGF and TrkA in melanoma, indicating a potential therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Vanessa M. Ruscetta, Taj J. J. Seaton, Aleen Shakeel, Stanley N. S. Vasconcelos, Russell D. D. Viirre, Marc J. J. Adler, Michael F. F. Olson
Summary: Cytoskeleton organization and dynamics are regulated by post-translational modifications of key target proteins, including the phosphorylation of myosin light chain proteins by MRCK kinases. Compared to ROCK kinases, the contributions of MRCK kinases are less characterized due to the late discovery of selective inhibitors. The recent development of selective MRCK inhibitors has expanded the tools to study MRCK function and shown therapeutic benefits in cancer studies.
Article
Plant Sciences
Weikun Jing, Yonghong Li, Shuai Zhang, Xiaofeng Zhou, Junping Gao, Nan Ma
Summary: Aquaporins (AQPs), as an ancient superfamily protein, play a crucial role in maintaining fluid homeostasis in organisms across different environments. This review summarizes the recent findings on the involvement of AQPs in plant cell signal transduction and highlights the unresolved issues for future investigation.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Neurosciences
Fan Zhang, Xinyue Lin, Aihua Liu, Jian Chen, Zhongyan Shan, Weiping Teng, Xiaohui Yu
Summary: Maternal subclinical hypothyroidism (SCH) during pregnancy can negatively impact the neurodevelopment and cognitive function of the offspring. Treatment with levothyroxine (L-T-4) may ameliorate cognitive impairment in SCH maternal rats' offspring by modulating nerve growth factor (NGF)-related signaling pathways.
MOLECULAR NEUROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Aziz M. Rangwala, Victoria R. Mingione, George Georghiou, Markus A. Seeliger
Summary: Phosphorylation plays a vital role in regulating biological processes, and protein kinases have been extensively studied for their involvement in human health and disease. Some kinases possess additional catalytic functions in addition to phosphotransferase activity, while others have lost their catalytic activity completely. In this study, we analyzed the UniProtKB database for bifunctional protein kinases and focused on those crucial for bacterial and human cellular homeostasis. These kinases have diverse functional roles in environmental sensing, metabolic regulation, immune-host defense, and cell cycle control. This article explores their dual catalytic activities and their contributions to disease pathogenesis.
Article
Biochemistry & Molecular Biology
Giovanna Testa, Marco Mainardi, Eleonora Vannini, Laura Pancrazi, Antonino Cattaneo, Mario Costa
Summary: The binding of nerve growth factor (NGF) to the receptors TrkA and p75(NTR) activates multiple pathways, regulating processes such as proliferation, differentiation, membrane potential, synaptic plasticity, and pain. In this study, PC12 cells lacking TrkA, p75(NTR), or both were created using CRISPR/Cas9 genome editing. TrkA-null cells were unresponsive to NGF, while the absence of p75(NTR) enhanced the phosphorylation of TrkA and its effectors. Activation of TrkA and p75(NTR) by NGF had antagonizing effects on the membrane potential, as demonstrated by patch-clamp experiments.
Article
Biochemical Research Methods
Harumi Kagiwada, Takayuki Kiboku, Hitomi Matsuo, Masashi Kitazawa, Kazuhiko Fukui, Katsuhisa Horimoto
Summary: Cellular protein phosphorylation is crucial for signal transduction, and the newly developed phosphorylation array system effectively assesses the activity of signaling pathways perturbed by external stimuli, offering a new approach to understanding intracellular signal transduction mechanisms.
Article
Biochemistry & Molecular Biology
Francesca Paoletti, Franci Merzel, Alberto Cassetta, Iza Ogris, Sonia Covaceuszach, Joze Grdadolnik, Doriano Lamba, Simona Golic Grdadolnik
Summary: NGF plays a crucial role in the maintenance and growth of neuronal populations, with interactions with ATP impacting the binding to specific receptors. The study suggests that ATP may serve as a transient molecular modulator of NGF signaling in health and disease states.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Review
Medicine, General & Internal
Jiawen Chen, Yafei Li, Chong Du, Tianwen Wei, Tiankai Shan, Liansheng Wang
Summary: Protein kinases play a crucial role in cardiovascular diseases and their study is of great significance for heart repair and treatment.
CHINESE MEDICAL JOURNAL
(2022)
Article
Cell Biology
Adrianne L. Stefanski, Michael D. Renecle, Anita Kramer, Shilpi Sehgal, Purnima Narasimhan, Kristen K. Rumer, Virginia D. Winn
Summary: This study demonstrates that Siglec-6 may act as a signaling molecule in the pregnancy-specific disorder preeclampsia, with phosphorylation by Src family kinases playing a crucial role in recruiting phosphatases like SHP-2 for downstream signaling.
Article
Pathology
Nathan Griffin, Fangfang Gao, Phillip Jobling, Christopher Oldmeadow, Vanessa Wills, Marjorie M. Walker, Sam Faulkner, Hubert Hondermarck
Summary: The study revealed that the expression of NGF and NGFR in esophageal cancer was similar to that in normal tissues, while NTRK1 expression was significantly higher in esophageal squamous cell carcinoma and lower in adenocarcinoma. The findings suggest that NTRK1 could serve as a potential biomarker and therapeutic target in esophageal squamous cell carcinoma.
Review
Pharmacology & Pharmacy
Giovanna Testa, Antonino Cattaneo, Simona Capsoni
Summary: NGF plays a crucial role in regulating pain transmission and perception in the nervous system. Mutations in the NGF gene can lead to different clinical manifestations, with R221W causing congenital insensitivity to pain but no cognitive impairments, while V232fs and R121W also result in intellectual disabilities.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Jingtong Zhao, Zhijun Luo
Summary: The Ras-Raf-MEK-ERK signaling pathway plays essential roles in cell proliferation, survival, differentiation, and development. The mechanisms of A-Raf and C-Raf activation are still not completely understood, while B-Raf is frequently mutated in many cancers. Understanding the regulation of Raf kinases is of great importance in tumorigenesis and cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yu-Lin Yang, Li -Bo Cao, Wen-Rui He, Li Zhong, Yi Guo, Qing Yang, Hong -Bing Shu, Ming-Ming Hu
Summary: The current understanding of nucleic acid-mediated innate immunity is that the binding of intracellular sensors to nucleic acids is enough to activate them. However, this study reveals that the endocytosis of viruses or foreign DNA provides a priming signal for the activation of the DNA sensor cGAS in the innate immune response. This finding demonstrates that the activation of cGAS following endocytosis requires tyrosine phosphorylation mediated by the V-ATPase-SYK pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Immunology
Weiyuan Wang, Melanie Cristina Lopez McDonald, Christine Kim, Mirielle Ma, Zetao (Tommy) Pan, Charlotte Kaufmann, David A. Frank
Summary: STATs are a family of transcription factors involved in various cellular processes. Dysregulation of STATs in tumors directly contributes to cancer pathogenesis. STAT1 suppresses tumor formation while STAT3 promotes tumor development. This review investigates the specific roles of STAT1 and STAT3 in both normal physiology and cancer biology, explores their interactions, and provides insights into therapeutic strategies by modulating their transcriptional activity.
FRONTIERS IN IMMUNOLOGY
(2023)