期刊
AMINO ACIDS
卷 37, 期 2, 页码 341-348出版社
SPRINGER WIEN
DOI: 10.1007/s00726-008-0159-x
关键词
Nanomaterials; Graphitic nanofibres (GNF); Derivatization; Mass spectrometry (MS); Matrix-assisted laser desorption/ionization (MALDI); Material-enhanced laser desorption/ionization (MELDI); Protein profiling
资金
- Genome Research in Austria (GEN-AU) (Federal Ministry for Education, Science and Culture, Vienna, Austria)
- West Austrian Initiative for Nano Networking (WINN)
- Austrian Science Foundation (FWF)
- SFB Project 021 (Vienna, Austria)
Graphitic nanofibres (GNFs), 100-200 nm in diameter and 5-20 mu m in length have been modified in order to yield different affinities (Cu2+ and Fe3+ loaded immobilized metal affinity chromatography (IMAC) as well as cation and anion exchange materials) for the extraction of a range of biomolecules by their inherited hydrophobicity and the hydrophilic chemical functionalities, obtained by derivatization. Modified GNFs have for the first time been employed as carrier materials for protein profiling in material-enhanced laser desorption/ionization (MELDI) for the enrichment and screening of biofluids. For that purpose, the derivatized GNF materials have comprehensively been characterized regarding surface area, structural changes during derivatization, IMAC, as well as ion exchange and protein-loading capacity and recovery. GNF derivatives revealed high protein-binding capacity (2,000 mu g ml(-1) for insulin) and ideal sensitivities, resulting in a detection limit of 50 fmol mu l(-1) (for insulin), which is crucial for the detection of low abundant species in biological samples. Compared to other MELDI carrier materials, sensitivity was enhanced on GNF derivatives, which might be ascribed to the fact that GNFs support desorption and ionization mechanisms and by absorbing laser energy in addition to matrix.
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