Article
Gastroenterology & Hepatology
Sa'ed H. Zyoud
Summary: This study provides the most up-to-date and comprehensive data on the trends in publications related to C.difficile infection. The number of published papers on this topic has significantly increased in the past decade. The United States is one of the countries with the highest publication rate. The study identifies three clusters of research areas, including illness spectrum and severity, laboratory diagnosis and characterization, and risk factors for C.difficile infection.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Microbiology
Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Deirdre A. Collins, Thomas V. Riley
Summary: This study evaluated the epidemiology, molecular characteristics, and antimicrobial susceptibility of C. difficile isolated from adults with diarrhoea in Vietnam. The overall prevalence of C. difficile was 15.1%, with 9.8% being toxigenic strains and 6.3% being non-toxigenic strains. The prevalence of multidrug resistance was 27.3%. Rating: 8 out of 10.
Article
Microbiology
Peng An Khun, Long Duc Phi, Phuong Thi Pham, Ha Thi Thu Nguyen, Quyen Thi Huyen Vu, Deirdre A. Collins, Thomas V. Riley
Summary: This study investigated the prevalence, molecular epidemiology, and antimicrobial susceptibility of Clostridioides difficile in children with diarrhea in Vietnam. The results showed a high prevalence of C. difficile, but a comparatively low proportion of toxigenic strains.
Article
Agriculture, Dairy & Animal Science
Melanie Werner, Patricia Eri Ishii, Rachel Pilla, Jonathan A. Lidbury, Joerg M. Steiner, Kathrin Busch-Hahn, Stefan Unterer, Jan S. Suchodolski
Summary: This study examined the relationship between gut bacteria imbalance and the presence of C. difficile in dogs. The results showed that dogs with C. difficile had a more severe imbalance in gut bacteria and lower levels of another bacteria (C. hiranonis). However, the presence of C. difficile did not affect the treatment response for digestive problems in dogs. Therefore, special treatment is not required for dogs carrying C. difficile.
Review
Medicine, General & Internal
Ibrahim A. Al-Zahrani
Summary: Clostridioides difficile (C. difficile) infection is a global threat to many healthcare settings. Excessive use of antimicrobials, lack of optimal antibiotic policies, and suboptimal infection control practices have fueled the development of this health issue. Prudent use of antimicrobials and simple infection control measures can significantly reduce infection rates.
SAUDI MEDICAL JOURNAL
(2023)
Article
Rehabilitation
Justin Weppner, Joelle Gabet, Mark Linsenmeyer, Mohamed Yassin, Gary Galang
Summary: The study found the presence of C difficile spores in an acute rehabilitation environment and showed that proper disinfection methods can effectively reduce the risk of infection. It is important to sporicidally disinfect surfaces such as beds and wheelchairs to decrease the rates of C difficile infection.
AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION
(2021)
Article
Medicine, General & Internal
Harris Carmichael, Steven M. Asch, Eran Bendavid
Summary: The study found that using antibiotics in patients with aURIs increases the occurrence of adverse events such as diarrhea, candidiasis, and Clostridium difficile infection, especially affecting female patients. Despite higher NNH compared to previous methods, the new analysis still shows that prescribing antibiotics in aURIs results in significant iatrogenic harm.
JOURNAL OF INTERNAL MEDICINE
(2023)
Article
Chemistry, Analytical
Waleed A. Hassanain, Julia Spoors, Christopher L. Johnson, Karen Faulds, Neil Keegan, Duncan Graham
Summary: The study introduces a novel testing platform for the rapid and simultaneous detection of two specific biomarkers of Clostridium difficile infection, providing a rapid, selective, sensitive, and cost-effective clinical evaluation method for CDI.
Article
Environmental Sciences
Eun Ju Oh, Jang Mook Kim, Jae Kyung Kim
Summary: This study analyzed the impact of climate factors on the incidence and severity of foodborne diseases, finding positive correlations between certain pathogens and variables such as sunshine intensity, wind-chill temperature, relative humidity, and particulate matter content. The findings may help predict relationships among climatic factors and inform environmental health policies, but further research involving large-scale data, molecular biology, and epidemiology is needed for effective prevention and management of foodborne bacterial infections.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Jianfeng Wang, Chu Yang, Chao Zhang, Xiaoyan Mao, An Lizhe
Summary: In this study, the genome of Clostridium difficile was studied using metagenomic technology, revealing its gene sequence information and potential toxicological genes. Through gene function analysis and pathogenicity analysis, the metabolism activity, catalytic activity, carbohydrate-active enzyme genes and other aspects of the bacterium were understood, and an assessment was made on its virulence genes.
Review
Multidisciplinary Sciences
Tebelay Dilnessa, Alem Getaneh, Workagegnehu Hailu, Feleke Moges, Baye Gelaw
Summary: A high prevalence of C. difficile was observed among hospitalized patients, emphasizing the need for attention and prevention. The resistance of C. difficile to metronidazole and vancomycin was lower compared to other antibiotics.
Review
Microbiology
Marta Mattana, Riccardo Tomasello, Claudia Cammarata, Paola Di Carlo, Teresa Fasciana, Giulio Giordano, Alessandro Lucchesi, Sergio Siragusa, Mariasanta Napolitano
Summary: This review analyzed the correlation between coagulation complications related to C. difficile infection and inflammasome activation, particularly the pyrin-dependent one. The evidence suggests that inflammasome activation increases the risk of venous thromboembolism, and inhibition of molecules involved in coagulation activation could block this process. It may be possible to reduce complications and mortality associated with C. difficile infection by reducing inflammatory activity and preventing thromboembolic complications.
Article
Agriculture, Dairy & Animal Science
Paul Dawson, Ahmet Buyukyavuz, Claudia Ionita, Julie Northcutt
Summary: The combination of PCR and DNA extraction techniques can be used to enumerate Campylobacter spp. from poultry gastrointestinal tract samples. The study developed a DNA standard curve for Campylobacter spp. DNA primers and a cell/genomic DNA extraction protocol for complex samples. The results showed that qPCR and standard plate count methods were highly correlated for enumerating Campylobacter spp. in the specified range.
Article
Multidisciplinary Sciences
Srijita Basak, Debashrito Deb, Utkarsh Narsaria, Tamalika Kar, Filippo Castiglione, Indraneel Sanyal, Pratap D. Bade, Anurag P. Srivastava
Summary: Clostridium difficile infection is a major health-associated infection with high incidence and mortality rates. A chimeric vaccine candidate was designed using immunoinformatics, which showed stability and reliability in silico and molecular dynamics simulations. Docking studies demonstrated stable interactions with immune receptors, and in silico modeling indicated competent expression in E. coli system for potential immune response.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Nguyen Thi Bui, Quyen Thi Huyen Vu, Luong Duy Trinh, Deirdre A. Collins, Thomas Riley
Summary: This study investigated the prevalence, molecular type, and antimicrobial susceptibility of Clostridioides difficile in the environment in Vietnam. It found that the overall contamination rate of C. difficile was 24.5%, with the highest prevalence in pig farm and hospital soils. C. difficile was also found in pig feces and potato surfaces. These findings highlight the importance of environmental sources in the epidemiology of C. difficile infection in Vietnam.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Evolutionary Biology
Krzysztof Fiedoruk, Magdalena Zakrzewska, Tamara Daniluk, Ewelina Piktel, Sylwia Chmielewska, Robert Bucki
GENOME BIOLOGY AND EVOLUTION
(2020)
Article
Nanoscience & Nanotechnology
Ewelina Piktel, Karolina H. Markiewicz, Agnieszka Z. Wilczewska, Tamara Daniluk, Sylwia Chmielewska, Katarzyna Niemirowicz-Laskowska, Joanna Mystkowska, Paulina Paprocka, Paul B. Savage, Robert Bucki
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2020)
Article
Pharmacology & Pharmacy
Sylwia Joanna Chmielewska, Karol Sklodowski, Joanna Depciuch, Piotr Deptula, Ewelina Piktel, Krzysztof Fiedoruk, Patrycja Kot, Paulina Paprocka, Kamila Fortunka, Tomasz Wollny, Przemyslaw Wolak, Magdalena Parlinska-Wojtan, Paul B. Savage, Robert Bucki
Summary: By linking cationic steroid antimicrobial ceragenin CSA-131 with gold nanoparticles Au NPs, a new class of potent nanoantibiotics with strong bactericidal activity against multidrug-resistant bacteria has been developed, showing potential for effective eradication of MDR strains.
Article
Biochemistry & Molecular Biology
Piotr Deptula, Lukasz Suprewicz, Tamara Daniluk, Andrzej Namiot, Sylwia Joanna Chmielewska, Urszula Daniluk, Dariusz Lebensztejn, Robert Bucki
Summary: This study focuses on the molecular mechanisms related to Helicobacter pylori infection and gastric cancer development. It suggests that the nanomechanical properties of infected tissues could serve as markers of H. pylori presence and the mechanical response of gastric cells to this infection may play a role in promoting cancer development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ewelina Piktel, Lukasz Suprewicz, Joanna Depciuch, Sylwia Chmielewska, Karol Sklodowski, Tamara Daniluk, Grzegorz Krol, Paulina Kolat-Brodecka, Piotr Bijak, Anna Pajor-Swierzy, Krzysztof Fiedoruk, Magdalena Parlinska-Wojtan, Robert Bucki
Summary: The study synthesized a series of varied-morphology gold nanoparticles with potent antibacterial activity against a spectrum of clinical strains, including Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus, and uropathogenic Escherichia coli. The optimized synthesis of gold nanoparticles allowed for the development of nanomaterials significantly more potent than previous formulations, with antimicrobial spectrum covering strains with different drug resistance mechanisms. The research highlights the potential for using non-spherical gold nanoparticles as new coatings targeting multidrug-resistant pathogens causing device-associated infections.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Suhanya Prasad, Ewelina Piktel, Joanna Depciuch, Alexey Maximenko, Lukasz Suprewicz, Tamara Daniluk, Jakub Spalek, Urszula Wnorowska, Piotr M Zielinski, Magdalena Parlinska-Wojtan, Paul B Savage, Slawomir Okla, Krzysztof Fiedoruk, Robert Bucki
Summary: The study showed that ceragenin-conjugated nonspherical gold nanoparticles have strong antibacterial and antibiofilm activity against common pathogens causing otitis media, with good biocompatibility and the ability to decrease IL-8 release from keratinocyte cells. This new treatment method has the potential to effectively eradicate biofilm-forming pathogens associated with otitis media.
Article
Pharmacology & Pharmacy
Karol Sklodowski, Sylwia Joanna Chmielewska, Joanna Depciuch, Piotr Deptula, Ewelina Piktel, Tamara Daniluk, Magdalena Zakrzewska, Michal Czarnowski, Mateusz Ciesluk, Bonita Durnas, Magdalena Parlinska-Wojtan, Paul B. Savage, Robert Bucki
Summary: Patients with chronic diabetes are more susceptible to infections.
Article
Microbiology
Jakub Spalek, Tamara Daniluk, Adrian Godlewski, Piotr Deptula, Urszula Wnorowska, Dominika Ziembicka, Mateusz Ciesluk, Krzysztof Fiedoruk, Michal Ciborowski, Adam Kretowski, Stanislaw Gozdz, Bonita Durnas, Paul B. Savage, Slawomir Okla, Robert Bucki
Summary: The study demonstrates that ceragenins have significant candidacidal activities in vitro, effectively eradicating Candida species responsible for voice prosthesis failure, with minimal development of resistance. Immersing VPs in ethanol solution containing CSA-131 results in silicon impregnation with ceragenin molecules and inhibits fungal biofilm formation on VP surfaces.
Review
Microbiology
Tomasz Wollny, Tamara Daniluk, Ewelina Piktel, Urszula Wnorowska, Anna Buklaha, Katarzyna Gluszek, Bonita Durnas, Robert Bucki
Summary: Irritable bowel syndrome (IBS) is a common, chronic, functional disorder that impacts a large proportion of the world population, with its pathophysiology not fully understood. Emerging evidence suggests that gut microbiota plays a significant role in the development of IBS, and therefore targeting microbial modulation may effectively enhance therapy.
Article
Biochemistry & Molecular Biology
Jakub Spalek, Przemyslaw Ociepa, Piotr Deptula, Ewelina Piktel, Tamara Daniluk, Grzegorz Krol, Stanislaw Gozdz, Robert Bucki, Slawomir Okla
Summary: This article describes the clinical application of different types of biomaterials in the field of otorhinolaryngology, focusing on their antimicrobial properties. Various biomaterials, such as ceramics and polymers, are widely used in ear, nose, and throat surgery. It is important to consider tissue restoration and prevention of microbial colonization when designing implant materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Joanna Tokajuk, Piotr Deptula, Sylwia J. Chmielewska, Karol Sklodowski, Zaneta A. Mierzejewska, Malgorzata Gradzka-Dahlke, Adam Tolstoj, Tamara Daniluk, Paulina Paprocka, Paul B. Savage, Robert Bucki
Summary: Ceragenin CSA-44 has been found to prevent biofilm formation on the surface of teeth and composite filling, as well as reduce the mass of established biofilm. This suggests that CSA-44 may serve as a potential agent in the development of new methods to combat oral pathogens and reduce the severity of oral infections.
Review
Biochemistry & Molecular Biology
Joanna Tokajuk, Piotr Deptula, Ewelina Piktel, Tamara Daniluk, Sylwia Chmielewska, Tomasz Wollny, Przemyslaw Wolak, Krzysztof Fiedoruk, Robert Bucki
Summary: The mechanisms for maintaining oral cavity homeostasis are influenced by environmental factors, and the oral microbiota and immune molecules play important roles. The LL-37 peptide is involved in maintaining microbial balance and is associated with the development of oral pathologies and systemic diseases.
Article
Environmental Sciences
Emilia Szumska, Przemyslaw Czajkowski, Michal Zablocki, Dorota Rozkiewicz
Summary: Hand hygiene is crucial in preventing nosocomial infections, yet some healthcare workers still practice improper and ineffective hand disinfection. Nurses exhibit better performance in properly disinfecting their hands compared to physicians, while long and artificial/polished nails are more commonly observed in nurses.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Medicine, Research & Experimental
Iwona Radziejewska, Malgorzata Borzym-Kluczyk, Katarzyna Leszczynska
Summary: Luteolin, a natural flavonoid, shows potential therapeutic effects on gastric cancer by reducing the expression levels of key factors like MUC1, sT antigen, and ADAM-17. It also induces the expression of inflammatory markers like IL-8 and cytokines such as IL-10 and NF-kappa B. The combination of luteolin and Helicobacter pylori seems to have a synergistic effect on suppressing gastric cancer progression.
BIOMEDICAL REPORTS
(2021)