期刊
AMERICAN JOURNAL OF VETERINARY RESEARCH
卷 71, 期 4, 页码 476-482出版社
AMER VETERINARY MEDICAL ASSOC
DOI: 10.2460/ajvr.71.4.476
关键词
-
资金
- Equine Health Studies Program, School of Veterinary Medicine, Louisiana State University
- CNPq - Brazil [201044/2005-6]
Objective-To identify differentially expressed genes in pulmonary tissues of horses affected with summer pasture-associated obstructive pulmonary disease (SPAOPD), which is a form of recurrent airway obstruction (RAO), compared with those of unaffected horses. Animals-6 horses with SPAOPD-RAO and 6 unaffected (healthy) horses. Procedures-Horses were assigned to 2 groups on the basis of medical history, clinical score, and transpleural pressure. Total RNA from each of the 5 lung lobes of each of the 6 SPAOPD-RAO-affected horses was extracted and pooled. Similarly, total RNA from unaffected horses was pooled. Differential display (DD) PCR assay was performed, and differentially expressed bands were purified and cloned into a plasmid vector. Plasmids were extracted from recombinant colonies, and purified DNA was sequenced. Genes of interest for RAO pathogenesis were identified. Real-time PCR assay was performed to confirm findings for the DD PCR assay. Results-18 differentially expressed genes (17 upregulated and 1 downregulated) were identified. Three genes of particular interest were found to be altered (2 upregulated and 1 downregulated) in horses with SPAOPD-RAO by use of real-time FOR assay, and these findings matched the differential expression found by use of the DD PCR assay. Conclusions and Clinical Relevance-SPAOPD-RAO in horses is a multifactorial, complex disease involving several genes. Upregulated genes, particularly beta 2-microglobulin, and the downregulated secretoglobin gene can serve as marker genes that may help to identify SPAOPD-RAO at an early age. (Am J Vet Res 2010;71:476-482)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据