期刊
AMERICAN JOURNAL OF VETERINARY RESEARCH
卷 70, 期 3, 页码 365-372出版社
AMER VETERINARY MEDICAL ASSOC
DOI: 10.2460/ajvr.70.3.365
关键词
-
资金
- USDA National Competitive Research [2004-01235]
Objective-To examine effects of in vitro exposure to solutions of hay dust, lipopolysaccharide (LPS), or beta-glucan on chemokine and cell-surface receptor (CSR) gene expression in primary bronchial epithelial cell cultures (BECCs) established from healthy horses and horses with recurrent airway obstruction (RAO). Sample Population-BECCs established from bronchial biopsy specimens of 6 RAO-affected horses and 6 healthy horses. Procedures-5-day-old BECCs were treated with PBS solution, hay dust solutions, LPS, or P-glucan for 6 or 24 hours. Gene expression of interleukin (IL)-8, chemokine (C-X-C motif) ligand 2 (CXCL2), IL-1 beta, toll-like receptor 2, toll-like receptor 4, IL-1 receptor 1, and glyceraldehyde 3-phosphate dehydrogenase was measured with a kinetic PCR assay. Results-Treatment with PBS solution for 6 or 24 hours was not associated with a significant difference in chemokine or CSR expression between BECCs from either group of horses. In all BECCs, treatment with hay dust or LPS for 6 hours increased IL-8, CXCL2, and IL-1 beta gene expression -> 3-fold; at 24 hours, only IL-1 beta expression was upregulated by > 3-fold. In all BECCs, CSR gene expression was not increased following any treatment. With the exception of a 3.7-fold upregulation of CXCL2 in BECCs from RAO-affected horses (following 6-hour hay dust treatment), no differences in chemokine or CSR gene expression were detected between the 2 groups. At 24 hours, CXCL2 gene expression in all BECCs was downregulated. Conclusions and Clinical Relevance-Epithelial CXCL2 upregulation in response to hay dust particulates may incite early airway neutrophilia in horses with RAO. (Am J Vet Res 2009;70:365-372)
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