Article
Cell Biology
Takuto Fujii, Mizuki Katoh, Manami Ootsubo, Oanh T. T. Nguyen, Mayumi Iguchi, Takahiro Shimizu, Yoshiaki Tabuchi, Yasuharu Shimizu, Hiroshi Takeshima, Hideki Sakai
Summary: CGs can inhibit glucose metabolism of cancer cells by inhibiting Na+,K+-ATPase and decreasing the expression of GLUT1. The binding of CGs with intracellular alpha 3NaK elicits NAADP-mediated calcium mobilization.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elena Arystarkhova, Mads S. Toustrup-Jensen, Rikke Holm, Jae-Kyun Ko, Kyung Eun Lee, Polina Feschenko, Laurie J. Ozelius, Allison Brashear, Bente Vilsen, Kathleen J. Sweadner
Summary: ATP1A3 encodes the alpha 3 isoform of Na,K-ATPase and is expressed only in neurons. Mutations in this gene result in a variety of phenotypes, with specific syndromes associated with unique substitutions. The pathogenicity of the R756H mutation was tested in mammalian cells and found to have reduced turnover rate, altered ion affinity, and resistance to misfolding.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Magda F. El-Adl, Taha M. El-Katony, Reham M. Nada
Summary: The study found that the specific ion effects of Na+ and K+ were different on the growth of Ulva lactuca. Ulva lactuca showed better growth with high concentrations of NaCl, while KCl might have adverse effects on photosynthesis and cell wall. Transcription of PM H+-ATPase was induced specifically by high concentrations of Na+, while the induction of PM-Na+/K+-ATPase-like alone could not improve the growth of KCl-treated Ulva lactuca.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Aline Yammine, Lizette Auezova, Gerard Lizard, Helene Greige-Gerges
Summary: The aim of this study was to investigate whether quercetin, resveratrol, or caffeic acid, at nano-and low micromolar concentrations, can improve the activity of Na+/K+- and Ca2+-ATPases in human erythrocyte membranes with excess cholesterol. These polyphenols belong to different chemical classes and are widely present in plant foods.
Article
Cell Biology
Rajkumar Rajanathan, Claudia Vilaseca i Riera, Tina Myhre Pedersen, Christian Staehr, Elena V. Bouzinova, Jens Randel Nyengaard, Morten B. Thomsen, Hans Erik Botker, Vladimir V. Matchkov
Summary: This study investigated the expression of two isoforms (alpha(1) and alpha(2)) of the Na+,K+-ATPase in the cardiovascular system, and the contribution of the alpha(2)-isoform to cardiac contractility. The study found that hearts with the alpha(+/G301R)(2) mutation exhibited greater contractility and a stronger response to ouabain, which was associated with increased systolic work.
Article
Neurosciences
Song Jiao, Kory Johnson, Cristina Moreno, Sho Yano, Miguel Holmgren
Summary: Research indicates that Na+/K+-ATPase α1 and α3 subunits are enriched in specific neuronal populations in the brain, with α3-expressing neurons playing a crucial role in cerebellar neuronal circuits. Furthermore, co-expression network analysis reveals key genes positively correlated with the expression of Na+/K+-ATPase α1 and α3.
JOURNAL OF COMPARATIVE NEUROLOGY
(2022)
Article
Plant Sciences
Qi Chen, Kai Xu, Yan Xu, Dehua Ji, Changsheng Chen, Chaotian Xie, Wenlei Wang
Summary: This study analyzed the function of Na+/K+-ATPase in the response of Neoporphyra haitanensis to salt stress and found that it played a crucial role in maintaining the K+/Na+ homeostasis in the algae. The inhibitor ouabain caused Na+ accumulation and K+ leakage in the cells, disrupting the K+/Na+ balance and leading to decreased photosynthesis and oxidative damage in the algae.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Marlene U. Fruergaard, Ingrid Dach, Jacob L. Andersen, Mette Ozol, Azadeh Shahsavar, Esben M. Quistgaard, Hanne Poulsen, Natalya U. Fedosova, Poul Nissen
Summary: In this study, the crystal structure and functional properties of the E2-BeFx complex of pig kidney Na+,K+-ATPase are investigated. The complex resembles the E2P intermediate and is able to bind ADP/ATP and Na+. The results suggest a relation between E2-BeFx and the E*P intermediate in the functional cycle of Na+, K+-ATPase.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Violetta V. Kravtsova, Arina A. Fedorova, Maria Tishkova, Alexandra A. Livanova, Oleg V. Vetrovoy, Alexander G. Markov, Vladimir V. Matchkov, Igor I. Krivoi
Summary: This study investigated the protective effect of ouabain on ionizing radiation-induced disturbances in skeletal muscle Na,K-ATPase function. Ouabain was found to prevent depolarization and loss of electrogenic contribution of the alpha 2 isozyme of Na,K-ATPase, accompanied by an increase in circulating ouabain concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Santiago E. Faraj, Wanda M. Valsecchi, Mariela Ferreira-Gomes, Mercedes Centeno, Elina Malen Saint Martin, Natalya U. Fedosova, Juan Pablo FC. Rossi, Monica R. Montes, Rolando C. Rossi
Summary: The Na+/K+-ATPase is a plasma membrane glycoprotein that exchanges intracellular Na+ for extracellular K+ by hydrolyzing ATP. The asymmetric distribution of Na+ and K+ is essential for cellular life. Current research indicates that Na+ becomes spontaneously occluded in the E1 dephosphorylated form of the Na+/K+-ATPase, showing positive interactions between binding sites. Adding ATP does not increase Na+ occlusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Microbiology
Cassiano Felippe Goncalves-de-Albuquerque, Carolina Medina Coeli da Cunha, Leo Victor Grimaldi de Castro, Caroline de Azevedo Martins, Marcos Roberto Colombo Barnese, Patricia Burth, Mauricio Younes-Ibrahim
Summary: In 1915, Inada and Ido identified Leptospira sp. as the pathogen responsible for Weil's Disease, which was later confirmed to cause leptospirosis. The molecular mechanisms and interactions between bacterial components and the host, as well as the systemic dysfunctions, still need to be fully understood. Leptospirosis affects all animal species and involves a cellular mechanism common to all eukaryotes. Na/K-ATPase is targeted by the leptospiral endotoxin, leading to dysfunctions and organ disorders. Additionally, metabolic disorders and inflammatory responses are triggered during leptospirosis, involving various molecular mechanisms. Na/K-ATPase plays a critical role in the disease and can be a potential therapeutic target or an indicator of disease severity.
Article
Cell Biology
Bruno Sterlini, Alessandra Romei, Chiara Parodi, Davide Aprile, Michele Oneto, Anita Aperia, Pierluigi Valente, Flavia Valtorta, Anna Fassio, Pietro Baldelli, Fabio Benfenati, Anna Corradi
Summary: This study reveals that PRRT2 deficiency affects the function of Na+/K+ ATPase (NKA) in neurons, leading to increased clustering of NKA on the plasma membrane, which can be reversed by re-expression of PRRT2. Additionally, PRRT2 deficiency also reduces the NKA-dependent afterhyperpolarization in neurons following high-frequency firing.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Qing Su, Xiao-Jing Yu, Xiao-Min Wang, Bo Peng, Juan Bai, Hong-Bao Li, Ying Li, Wen-Jie Xia, Li-Yan Fu, Kai-Li Liu, Jin-Jun Liu, Yu-Ming Kang
Summary: Numerous studies have shown that a high salt diet can inhibit brain Na+/K+-ATPase activity and induce oxidative stress and inflammation in the paraventricular nucleus (PVN). This study explores the role of Na+/K+-ATPase alpha 2-isoform (NKA alpha 2) in regulating oxidative stress and inflammation in the PVN during salt-induced hypertension. The results suggest that inhibiting NKA alpha 2 can decrease oxidative stress and inflammation, leading to reduced sympathetic activity in rats with salt-induced hypertension.
Article
Biochemistry & Molecular Biology
Violetta V. Kravtsova, Arina A. Fedorova, Maria Tishkova, Alexandra A. Livanova, Viacheslav O. Matytsin, Viacheslav P. Ganapolsky, Oleg V. Vetrovoy, Igor I. Krivoi
Summary: The Na,K-ATPase plays a crucial role in adaptation to hypoxia, and hypoxic preconditioning can protect against functional impairments. Mild hypoxia was found to modulate the Na,K-ATPase and improve skeletal muscle electrogenesis, which may have implications for countermeasures in disuse-induced pathology and hypoxic therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Jin Zheng, Ping Lan, Meihe Li, Min-chao Kang, Meng Xun, Xiangyun Ma, Mengyao Yan, Dan Sun, Yu Shen, Xinyi Fu, Xiaoming Ding, Xiaofei Yan, Wu-jun Xue
Summary: Reduced Na+/K+-ATPase (NKA) activity and NKA alpha 1 expression are implicated in the pathogenesis of renal diseases. NKA alpha 1 deficiency exacerbates tubulointerstitial lesions and fibrosis in a mouse model of UUO. Activation of NKA alpha 1 by an antibody against its extracellular DR region (DRm217) prevents UUO-induced tubulointerstitial lesions, preserves kidney function, and reduces renal fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Veterinary Sciences
E. Jose-Cunilleras, I. D. Wijnberg
EQUINE VETERINARY JOURNAL
(2016)
Letter
Physiology
Eric van Breda, Paul F. M. Schoffelen, Guy Plasqui
JOURNAL OF APPLIED PHYSIOLOGY
(2017)
Letter
Hematology
Eric van Breda, Steven Truijen
LANCET HAEMATOLOGY
(2017)
Letter
Hematology
Jacob Bejder, Lars Nybo, Michael N. Sawka, Michael J. Joyner, Nikolai Baastrup Nordsborg
LANCET HAEMATOLOGY
(2017)
Review
Oncology
Vesile Yildiz Kabak, Patrick Calders, Tulin Duger, Jibril Mohammed, Eric van Breda
SUPPORTIVE CARE IN CANCER
(2019)
Article
Sport Sciences
Paul F. M. Schoffelen, Marcel den Hoed, Eric van Breda, Guy Plasqui
SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS
(2019)
Review
Oncology
Nick Gebruers, Melissa Camberlin, Fleur Theunissen, Wiebren Tjalma, Hanne Verbelen, Timia Van Soom, Eric van Breda
SUPPORTIVE CARE IN CANCER
(2019)
Review
Clinical Neurology
Joris Lemmens, Joke De Pauw, Timia Van Soom, Sarah Michiels, Jan Versijpt, Eric van Breda, Rene Castien, Willem De Hertogh
JOURNAL OF HEADACHE AND PAIN
(2019)
Article
Sport Sciences
Thomas Peeters, Eric van Breda, Wim Saeys, Evi Schaerlaken, Jochen Vleugels, Steven Truijen, Stijn Verwulgen
INTERNATIONAL JOURNAL OF SPORTS MEDICINE
(2019)
Article
Veterinary Sciences
Wilhelmina Bergmann, Marjolijn de Mik-van Mourik, Stefanie Veraa, Jan van den Broek, Inge D. Wijnberg, Willem Back, Andrea Grone
EQUINE VETERINARY JOURNAL
(2020)
Article
Veterinary Sciences
C. Graubner, W. Bergmann, V Gerber, S. Veraa, A. Oevermann, I. D. Wijnberg
SCHWEIZER ARCHIV FUR TIERHEILKUNDE
(2020)
Article
Multidisciplinary Sciences
Gijsbert J. Jansen, Marit Wiersma, Willem J. B. van Wamel, Inge D. Wijnberg
Summary: A new method using saliva samples for detecting SARS-CoV-2 can distinguish between the presence and activity of the virus, potentially providing valuable information in the qualitative results of the virus.
Article
Pathology
Wilhelmina Bergmann, Chris van de Lest, Saskia Plomp, Johannes C. M. Vernooij, Inge D. Wijnberg, Willem Back, Andrea Grone, Mark W. Delany, Nermin Caliskan, Marianna A. Tryfonidou, Guy C. M. Grinwis
Summary: The gross morphology of intervertebral discs in horses is similar to dogs and humans, but there are differences in the biochemical composition and histological changes. Age and degeneration are associated with advanced glycation end-products (AGEs) and fibrosis. Unlike dogs and humans, there is no consistent difference in glycosaminoglycan content and hydration between the annulus fibrosus (AF) and nucleus pulposus (NP) in horses. Degeneration starts in the NP, indicated by decreased hydroxylysine and increased collagen 1 and AGEs. Increased AGE deposition and collagen content in the caudal cervical NP suggest a higher risk for spinal diseases in this area.
VETERINARY PATHOLOGY
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
Steven Truijen, Wim Saeys, Erik Haring, Hilde Feys, Emilie Meyvis, Anouche Van den Eynde, Kristof Vaes, Eric van Breda, Evi Schaerlaken, Stijn Verwulgen
ADVANCES IN USABILITY AND USER EXPERIENCE
(2020)
Article
Obstetrics & Gynecology
T. Van Soom, W. Tjalma, S. El Bakkali, H. Verbelen, N. Gebruers, E. van Breda
FACTS VIEWS AND VISION IN OBGYN
(2018)