Article
Engineering, Environmental
Juan Manuel Gutierrez-Villagomez, Geraldine Patey, Tuan Anh To, Molly Lefebvre-Raine, Linda Ramona Lara-Jacobo, Jerome Comte, Bert Klein, Valerie S. Langlois
Summary: The research revealed that while Bti biopesticides had limited impact on certain parameters of Lithobates sylvaticus and Anaxyrus americanus tadpoles, they might affect the time to metamorphosis and the intestinal microbiota composition.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Entomology
David Fernandez-Chapa, Hugo Alberto Luna-Olvera, Jesica Ramirez-Villalobos, Guadalupe Rojas-Verde, Katiushka Arevalo-Nino, Luis Jesus Galan-Wong
Summary: The study evaluated the biological activity of reconstituted Bti endotoxins after 40 years of storage against Aedes aegypti larvae. All reconstituted endotoxins from stored extracts showed an increase in potency. Protein profiles in polyacrylamide gels revealed a greater presence of Cyt toxins compared to the stored Bti extracts, which maintained their activity at high concentrations.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2021)
Article
Entomology
Nantipat Chimkhan, Sutticha Na-Ranong Thammasittirong, Sittiruk Roytrakul, Sucheewin Krobthong, Anon Thammasittirong
Summary: This study investigated the molecular responses of Aedes aegypti larvae to green synthesized silver/silver chloride nanoparticles (Ag/AgCl NPs). The results showed that Ag/AgCl NPs could cause mitochondrial dysfunction, DNA and protein damage, inhibition of cell proliferation, and cell apoptosis in A. aegypti larvae. These findings provide insights into the mechanisms of action of Ag/AgCl NPs on the control of A. aegypti larvae.
Article
Parasitology
Norbert Becker, Sophie Min Langentepe-Kong, Artin Tokatlian Rodriguez, Thin Thin Oo, Dirk Reichle, Renke Luehken, Jonas Schmidt-Chanasit, Peter Luethy, Arianna Puggioli, Romeo Bellini
Summary: This study shows that an integrated control program consisting of community participation, door-to-door intervention, and the sterile insect technique can effectively control the population of Aedes albopictus. The use of Bacillus thuringiensis israelensis-based fizzy tablets and water-dispersible granular formulation is the most effective intervention in reducing the population, while the mass release of sterile male mosquitoes also yields good results.
PARASITES & VECTORS
(2022)
Review
Immunology
Intikhab Alam, Khadija Batool, Aisha Lawan Idris, Weilong Tan, Xiong Guan, Lingling Zhang
Summary: This review summarizes the functional role of C-type lectins in Ae. aegypti mosquitoes and the mechanism by which CTLs interfere with the interaction between Cry toxins and receptors, altering Cry toxicity. Additionally, future research directions on elucidating the Bti resistance mechanism are outlined. This study provides a foundation for understanding Bti resistance and developing novel insecticides.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agronomy
Angela F. Harris, Jose Sanchez Prats, Nicole Nazario Maldonado, Cesar Piovanetti Fiol, Marla Garcia Perez, Patricia Ramirez-Vera, Julieanne Miranda-Bermudez, Marianyoly Ortiz, Peter DeChant
Summary: The study in Puerto Rico found that repeated wide-area larvicide spray applications of Bti AM65-52 WDG effectively suppressed the Ae. aegypti population, resulting in a reduction in adult female mosquito numbers. The success of this trial has led to the expansion of the program to other municipalities in Puerto Rico.
PEST MANAGEMENT SCIENCE
(2021)
Article
Public, Environmental & Occupational Health
Steven Gowelo, Paola Meijer, Tinashe Tizifa, Tumaini Malenga, Monicah M. Mburu, Alinune N. Kabaghe, Dianne J. Terlouw, Michele van Vugt, Kamija S. Phiri, Themba Mzilahowa, Constantianus J. M. Koenraadt, Henk van den Berg, Lucinda Manda-Taylor, Robert S. McCann, Willem Takken
Summary: Larval source management (LSM) is an effective strategy to reduce malaria transmission, and involving communities in LSM can increase intervention coverage and promote sustainability. In this study, community-led LSM activities were carried out in 26 villages in Malawi, resulting in a reduction in anopheline larval densities. However, the impact of community-led LSM could not be detected due to low mosquito densities after the scale-up of core malaria control interventions. The study highlights the potential benefits of increasing community knowledge about malaria and its control methods.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2023)
Article
Chemistry, Multidisciplinary
Hadi Loutfi, Nancy Fayad, Fabrice Pellen, Bernard Le Jeune, Maissa Chakroun, Dalel Benfarhat, Roger Lteif, Mireille Kallassy, Guy Le Brun, Marie Abboud
Summary: This paper reports a morphological study of crystals and spores synthesized by seven different strains of Bacillus thuringiensis, revealing variations in size distribution and morphology among the strains. The research suggests that there may be unexplored differences between strains of the same serovar, indicating that they may be less identical than previously believed.
APPLIED SCIENCES-BASEL
(2021)
Article
Environmental Sciences
Emmanuel Hakizimana, Chantal Marie Ingabire, Alexis Rulisa, Fredrick Kateera, Bart van den Borne, Claude Mambo Muvunyi, Michele van Vugt, Leon Mutesa, Gebbiena M. Bron, Willem Takken, Constantianus J. M. Koenraadt
Summary: This study evaluated the entomological impact of community-based application of Bacillus thuringiensis var. israelensis (Bti) in a rice irrigation scheme. The results showed that the community-led implementation program had a greater reduction in Anopheles larval habitats compared to the program supervised by the project team and the control group, and almost completely prevented pupal production.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Food Science & Technology
Khadija Batool, Intikhab Alam, Peiwen Liu, Zeng Shu, Siyu Zhao, Wenqiang Yang, Xiao Jie, Jinbao Gu, Xiao-Guang Chen
Summary: In this study, we improved the mosquitocidal efficiency of AeDNV by introducing Bti toxin peptides into its genome. The recombinant virus showed higher pathogenic activity and stability compared to the wild-type virus.
Article
Entomology
Joseph Davis, Christopher S. Bibbs, Gunter C. Muller, Rui-De Xue
Summary: The attractive toxic sugar baits (ATSB) are a novel and effective mosquito control tool that targets adult Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus based on their sugar-feeding behaviors and oral ingestion. Bacillus thuringiensis israelensis (BTi) shows promising results as an active ingredient in toxic sugar baits (TSB), with high mortality rates for the targeted mosquito species. The study suggests that ingestible BTi TSBs could be a viable alternative to current mosquito control strategies for these three species of mosquitoes.
JOURNAL OF VECTOR ECOLOGY
(2021)
Article
Environmental Sciences
Verena Gerstle, Alessandro Manfrin, Sara Kolbenschlag, Maximilian Gerken, A. S. M. Mufachcher Ul Islam, Martin H. Entling, Mirco Bundschuh, Carsten A. Bruehl
Summary: This study found that Bti may indirectly affect the abundance of dragonflies by reducing the number of chironomid larvae, and also have an impact on the composition of aquatic macroinvertebrate communities. As dragonflies include species of conservation concern, the necessity of Bti applications in habitats such as floodplains should be carefully evaluated.
ENVIRONMENTAL POLLUTION
(2023)
Article
Entomology
T. Sarita Achari, Chinmayee Panda, Tapan Kumar Barik
Summary: The study examined the impact of high temperatures and Bti insecticide on the biochemical profile of Aedes aegypti and Aedes albopictus mosquitoes. Both temperature and Bti significantly influenced the level of protein and enzyme activities in both mosquito species. Enzymes were highly expressed in Ae. aegypti compared to Ae. albopictus, suggesting the importance of considering environmental factors like temperature in evaluating the efficacy of Bti on Aedes mosquitoes.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2022)
Article
Parasitology
Jiangyu Wu, Li Wei, Jiali He, Kang Fu, Xinxin Li, Luyu Jia, Ruiping Wang, Wenfei Zhang
Summary: The novel mosquitocidal toxin Xpp81Aa1, identified from Bacillus thuringiensis strain HSY204, has significant toxicity to Aedes aegypti larvae, shows little similarity to traditional Cry and Cyt toxins, and exhibits a synergistic effect when combined with Cry2Aa and Cry4Aa protein toxins. Safety assessment revealed no cytocidal activity or allergic reactions, making Xpp81Aa1 a promising candidate for mosquito control applications to reduce mosquito-borne diseases.
Article
Entomology
Valerie M. Anderson, Grace F. H. Sward, Christopher M. Ranger, Michael E. Reding, Luis Canas
Summary: The study showed that incorporating Bti into straw substrate can effectively reduce the population of fungus gnats, while S. feltiae was generally ineffective. Bti did not have a negative impact on the growth of oyster mushrooms.