期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 84, 期 2, 页码 35-42出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2011.09-0507
关键词
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资金
- Instituto Colombiano Francisco Jose de Caldas para la Ciencia y la Tecnologia COLCIENCIAS
- Colombian Ministry for Social Protection [216-2006]
- Health Department of the Valle del Cauca, Cali, Colombia
- Tropical Medicine Research Center (TMRC-Cali) (NIAID) [49486-01]
- Brazilian Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Plasmodium vivax circumsporozoite (CS) protein is a leading malaria vaccine candidate. We describe the characterization of specific immune responses induced in 21 malaria-naive volunteers vaccinated with long synthetic peptides derived from the CS protein formulated in Montanide ISA 720. Both antibody- and cell-mediated immune responses were analyzed. Antibodies were predominantly of IgG1 and IgG3 isotypes, recognized parasite proteins on the immunofluorescent antibody test, and partially blocked sporozoite invasion of hepatoma cell lines in vitro. Peripheral blood mononuclear cells from most volunteers (94%) showed IFN-gamma production in vitro upon stimulation with both long signal peptide and short peptides containing CD8+ T-cell epitopes. The relatively limited sample size did not allow conclusions about HLA associations with the immune responses observed. In summary, the inherent safety and tolerability together with strong antibody responses, invasion blocking activity, and the IFN-gamma production induced by these vaccine candidates warrants further testing in a phase II clinical trial.
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