期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 84, 期 2, 页码 28-34出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2011.09-0499
关键词
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资金
- WHO-IVR
- World Health Organization [LA-35735G]
- National Institute of Allergy and Infectious Diseases through Tropical Medicine Research Centers [49486]
- National Heart, Lung and Blood Institute (NHLBI), Bethesda
- COLCIENCIAS
- Colombian Ministry for Social Protection [253-2005, 207-2007]
- International Center of Excellence for Malaria Research NIAID/ICEMR [U 19AI089702]
Vaccine development for Plasmodium vivax malaria is underway. A model to assess the protective efficacy of vaccine candidates in humans is urgently needed. Given the lack of continuous P vivax cultures, we developed a system to infect Anopheles albimanus mosquitoes using blood from P vivax-infected patients and determined parameters for challenge of malaria-naive volunteers by mosquito bite. Absence of co-infections in parasitized blood was confirmed by tests consistent with blood bank screening. A total of 119 experiments were conducted using batches of 900-4,500 mosquitoes fed by an artificial membrane feeding method. Optimal conditions for mosquito probing and infection were determined. Presence of oocyst and sporozoites were assessed on Days 7-8 and 14-15, respectively, and conditions to choose batches of infected mosquitoes for sporozoite challenge were established. Procedures to infect volunteers took a 2-hour period including verification of inoculum dose. Anopheles albimanus mosquitoes represent a valuable resource for P. vivax sporozoite challenge of volunteers.
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