期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 83, 期 5, 页码 1146-1155出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2010.10-0212
关键词
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We evaluated performance of three commercial Japanese encephalitis virus (JEV) IgM antibody capture enzyme linked immunosorbent assay (MAC ELISA) kits with a panel of serological specimens collected during a surveil lance project of acute encephalitis syndrome in India and acute meningitis and encephalitis syndrome in Bangladesh The serum and cerebral spinal fluid specimens had been referred to the Centers for Disease Control and Prevention (CDC) for confirmatory testing The CDC results and specimen classifications were considered the reference standard All three commercial kits had high specificity (95-99 5%), but low sensitivities ranging from 17-57% with both serum and cerebro spinal fluid samples Specific factors contributing to low sensitivity compared with the CDC ELISA could not be determined through further analysis of the limits and dilution end points of IgM detection
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