期刊
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
卷 80, 期 2, 页码 294-301出版社
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2009.80.294
关键词
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资金
- U.S. Public Health Service [A133096]
- James P. Nataro and Danish Council for Strategic Research [2101-07-0023]
- Statens Serum Institut, Copenhagen, Denmark
Enteroaggregative Escherichia coli (EAEC) pathogenesis is thought to comprise intestinal colonization followed by the release of enterotoxins and cytotoxins. Here, we use a polymerase chain reaction (PCR) to determine the prevalence of 10 genes encoding serine protease autotransporter toxins (SPATEs) in a collection of clinical EAEC isolates. Eighty-six percent of EAEC strains harbored genes encoding one or more class I cytotoxic SPATE proteins (Pet, Sat. EspP, or SigA). Two Class II, non-cytotoxic, SPATE genes were found among EAEC strains: pic and sepA, each originally described in Shigella flexneri 2a. Using a multiplex PCR for five SPATE genes (pet, sat, sigA, pic, and sepA), we found that most of the Shigella isolates also harbored more than one SPATE, whereas members of most other E. coli pathotypes rarely harbored a cytotoxic SPATE gene. SPATEs may be relevant to the pathogenesis of both EAEC and Shigella spp.
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