Article
Medical Laboratory Technology
Morgan Lundgren, Peter Ridefelt, Mathias Karlsson, Anna Norling, Anders Larsson
Summary: NT-proBNP is commonly used to rule out heart failure, but there is limited standardization between manufacturers, leading to different treatment of patients. A common calibrator and reference materials are needed to address this issue.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medicine, Research & Experimental
Meichun Zeng, Qingjun Jia, Jingjing Chen, Liming Xu, Li Xie, Qinglin Cheng, Qingchun Li, Ming Xiao, Zijian Fang
Summary: The relationship between plasma EBV-DNA load (PEDL) and Epstein-Barr virus (EBV)-encoded small RNA (EBER) during the early treatment of lymphoma is not well-established. This study found that although EBER affects the levels of PEDL, there is poor concordance between EBER and qualitative analysis of PEDL (qPEDL).
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Article
Oncology
Xiao-Hui Zheng, Xi-Zhao Li, Ting Zhou, Cheng-Tao Jiang, Cao-Li Tang, Chang-Mi Deng, Ying Liao, Yong-Qiao He, Tong-Min Wang, Wei-Hua Jia
Summary: Detecting EBV DNA load in nasopharyngeal brushing samples has attracted attention for the diagnosis of nasopharyngeal carcinoma. The diagnostic performance of EBV DNA methylation is less affected by brush sampling methods, showing great potential for the diagnosis of NPC.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Microbiology
Kenneth Gavina, Lauren C. Franco, Christopher M. Robinson, Weston Hymas, Guang-Sheng Lei, Will Sinclair, Tara Hall, John Carlquist, John-Paul Lavik, Christopher L. Emery, Phillip R. Heaton, David Hillyard, Bert K. Lopransi, Ryan F. Relich
Summary: The demand for SARS-CoV-2 testing during the COVID-19 pandemic has led to a wide range of analytical tests with different approaches and designs. The lack of standardized reference materials early in the pandemic has created challenges in harmonizing viral quantitation data.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
K. Mourik, S. A. Boers, A. L. van Rijn, J. C. P. Thijssen, R. Doorn, S. Svraka, A. Bart, E. Wessels, E. C. J. Claas, R. H. T. Nijhuis
Summary: This study evaluated the qualitative and quantitative performance of the NeuMoDx CMV and EBV assays compared to lab developed tests (LDT) and assays on the Abbott m2000 system. The results showed that the NeuMoDx assays are suitable for medium-to-high throughput diagnostic settings, but there are differences in sensitivity and quantification compared to the Abbott m2000 system, especially for EBV.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Medicine, General & Internal
Justyna Moppert, Krzysztof Domagalski, Sylwia Wrotek, Malgorzata Pawlowska
Summary: This study aimed to evaluate the predictive role of TNF-α, IL-6, s-ICAM-1, and EBV DNA load levels in hepatological complications of EBV infection in children. The findings revealed that elevated concentrations of IL-6, TNF-α, and s-ICAM-1 may indicate the risk of hepatitis with concomitant biliary pole damage.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Virology
Zhikai Wan, Ying Chen, Jiangjin Hui, Yongzheng Guo, Xiaorong Peng, Mengyan Wang, Caiqin Hu, Yirui Xie, Junwei Su, Ying Huang, Xiaoke Xu, Yan Xu, Biao Zhu
Summary: This study analyzed the characteristics of Epstein-Barr Virus (EBV) infection in patients infected with human immunodeficiency virus-1 (HIV) in southeastern China. The results showed that EBV-1 was the main strain in HIV-infected patients, and EBV-2 had a higher viral load. HIV-infected patients had higher active virus replication and different mutations of EBV LMP-1 C-ter compared to non-HIV-infected patients.
Article
Pathology
Kseniya Petrova-Drus, Andres E. Quesada, Anita S. Bowman, Ryan Ptashkin, Jinjuan Yao, Maria E. Arcila, Caleb Ho, Christine Moung, Jennifer Regalado, Ryma Benayed, Jamal K. Benhamida, Pallavi K. Galera, Ahmet Dogan, Chad Vanderbilt
Summary: Using hybridization capture-based next-generation sequencing, EBV-related viral DNA can be effectively detected in patients with hematological neoplasms. The study showed a strong correlation between the quantitation of EBV reads by NGS and EBV-encoded RNA in situ hybridization, particularly in cases with significant EBV involvement. This approach provides a valuable tool for diagnosing and monitoring EBV-associated conditions in patients with hematologic malignancies.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Biochemical Research Methods
Alison Devonshire, Gerwyn Jones, Ana Fernandez Gonzalez, Olga Kofanova, Johanna Trouet, Pamela Pinzani, Stefania Gelmini, Serena Bonin, Carole Foy
Summary: The analysis of circulating cell-free DNA (ccfDNA) in liquid biopsies is becoming increasingly important in clinical practice. However, the accuracy of diagnosis is greatly impacted by the quality of the samples, and standardized approaches for assessing the quality of ccfDNA have not yet been established. This study evaluates the use of nucleic acid spike-in control materials as a quality control (QC) and standardization method for cfDNA isolation in in vitro diagnostic assays.
Article
Oncology
Juliane Bluemke, Marcus Bauer, Christoforos Vaxevanis, Ofer Mandelboim, Andreas Wilfer, Claudia Wickenhauser, Barbara Seliger, Simon Jasinski-Bergner
Summary: The human Epstein-Barr virus (EBV) is associated with different tumor types and encodes 48 different microRNAs (miRs). Certain EBV-miRs play a role in immune evasion mechanisms of infected cells, and ebv-miR-BART7-3p can downregulate IFNL3 expression.
Article
Virology
Hala Abdullatif, Anil Dhawan, Anita Verma
Summary: Infections after liver transplantation, especially viral infections, have a significant impact on graft function and overall outcome. The study reviewed the epidemiology and risk factors of EBV, CMV, and non-EBV non-CMV viral infections in pediatric liver transplant recipients. The majority of infections were viral in origin, with EBV viremia having the highest incidence. Older donor age, auxiliary graft, and bacterial infections were associated with EBV infection, while younger recipient age, D+R- CMV IgG, and left lateral segment graft were risk factors for CMV infection. Non-EBV and CMV viral infections did not contribute to increased complications or rejection, indicating the importance of identifying and managing these infections in pediatric LT recipients.
Article
Multidisciplinary Sciences
Maria Pena-Francesch, Liliana Danusia Vanoaica, Gao-Feng Zhu, Michael Stumpe, Devanarayanan Siva Sankar, Heike Nowag, Alma Delia Valencia-Camargo, Wolfgang Hammerschmidt, Jarn Dengjel, Laure-Anne Ligeon, Christian Munz
Summary: Autophagy serves as a defense mechanism against intracellular pathogens, but certain viruses, including EBV, exploit it to their advantage. In this study, the researchers found components of the autophagy machinery and viral proteins in purified EBV virions. They also discovered that BVRF2 and BdRF1 interact with LC3B-II and are crucial for capsid assembly and envelope release, respectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Engineering, Environmental
Mano Sivaganesan, Jessica R. Willis, Mohammad Karim, Akin Babatola, David Catoe, Alexandria B. Boehm, Maxwell Wilder, Hyatt Green, Aldo Lobos, Valerie J. Harwood, Stephanie Hertel, Regina Klepikow, Mondraya F. Howard, Pongpan Laksanalamai, Alexis Roundtree, Mia Mattioli, Stephanie Eytcheson, Marirosa Molina, Molly Lane, Richard Rediske, Amanda Ronan, Nishita D'Souza, Joan B. Rose, Abhilasha Shrestha, Catherine Hoar, Andrea I. Silverman, Wyatt Faulkner, Kathleen Wickman, Jason G. Kralj, Stephanie L. Servetas, Monique E. Hunter, Scott A. Jackson, Orin C. Shanks
Summary: Surface water quality qPCR technologies are becoming routine in environmental and public health labs, but the need for reliable reference materials to interpret measurements across labs is crucial. The study investigates the performance of a DNA plasmid construct, SRM 2917, in 12 qPCR assays across 14 different labs. Bayesian analysis is used to generate assay-specific calibration models and assess data acceptance metrics. The results show that SRM 2917 allows for reproducible calibration models and offers options for minimizing variability and improving comparability of data across labs.
Review
Cell Biology
Nicole L. Diggins, Meaghan H. Hancock
Summary: Viruses use various mechanisms to overcome barriers to infection in host cells. One such mechanism is the use of virally-encoded microRNAs (miRNAs) to regulate host gene expression for the benefit of the virus. Despite little sequence homology, different viruses target common cellular pathways such as apoptosis, immune evasion, cell growth, and differentiation. This article highlights the importance of viral miRNAs in lytic and latent infections by targeting these common cellular processes and provides insights into their mimicry of host cell miRNAs and regulation of host miRNA expression.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
A. Sarah Walker, Emma Pritchard, Thomas House, Julie Robotham, Paul J. Birrell, Iain Bell, John Bell, John N. Newton, Jeremy Farrar, Ian Diamond, Ruth Studley, Jodie Hay, Karina-Doris Vihta, Timothy E. A. Peto, Nicole Stoesser, Philippa C. Matthews, David W. Eyre, Koen B. Pouwels
Summary: The study found marked variation in community SARS-CoV-2 Ct values, suggesting that they could be a useful epidemiological early-warning indicator.