Article
Pharmacology & Pharmacy
Zibei Lin, Long Xi, Shaokui Chen, Jinsong Tao, Yan Wang, Xin Chen, Ping Li, Zhenping Wang, Ying Zheng
Summary: The study demonstrates that different sizes of PLGA nanoparticles can affect their uptake by dendritic cells and their trafficking in the skin and lymphoid system. Larger particles are more readily taken up by dendritic cells and are captured and transported more efficiently in vivo.
ACTA PHARMACEUTICA SINICA B
(2021)
Review
Pharmacology & Pharmacy
Preeti Sharma, Alakesh Alakesh, Siddharth Jhunjhunwala
Summary: Particle-based systems are widely used in the clinic, but internalization of particles may have unintended effects on immune cell functions. Understanding the impact of particles on immune cells is crucial for designing biocompatible and efficient particle formulations.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2022)
Article
Medicine, Research & Experimental
Paul R. Hartmeier, Jessica L. Kosanovich, Ketki Y. Velankar, Jennifer Armen-Luke, Madeline A. Lipp, Ellen S. Gawalt, Nick Giannoukakis, Kerry M. Empey, Wilson S. Meng
Summary: This study used biotin-modified PLGA nanoparticles to deliver proteins and stimulate professional antigen-presenting cells. The results showed that these nanoparticles were capable of retaining proteins and had self-adjuvant properties, inducing specific antigen-presenting cell responses. In addition, bNPs demonstrated feasibility and effectiveness in vivo.
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Multidisciplinary
Hongbin Wang, Xiaona Li, Jing Wang, Jiannan Wang, Hongyan Zou, Xuesong Hu, Linqing Yang, Penghui Shen, A. Rong, Kaiqi Wang, Yingbo Li, Jie Yang, Kai Wang, Lili Yang, Lina Wu, Xilin Sun
Summary: Recent advances in intratracheal delivery strategies have generated significant interest in the field of biomedical research for the diagnosis and treatment of lung cancer. However, there is a lack of studies investigating the behavior and mechanism of imaging nanoparticles (NPs) after intratracheal delivery. This study demonstrates that nanosized perfluoro-15-crown-5-ether (PFCE NPs) show enhanced 19F-MRI signal-to-noise ratio (SNR) compared to perfluorooctyl bromide (PFOB NPs) up to day 7 after intratracheal delivery. Alveolar macrophages (AMs) engulf PFCE NPs, migrate to the tumor margin, and increase tumor PFCE concentration and 19F-MRI signals. The translocation of PFCE NPs by AMs to lung draining lymph nodes (dLNs) reduces background PFCE concentration. These findings provide insights into the dynamic AMs-mediated translocation of intratracheally delivered PFC NPs for effective lung tumor visualization, suggesting a pathway for the clinical translation of an intratracheal delivery-based imaging strategy.
Review
Biochemistry & Molecular Biology
Marcelle Mehu, Chandrakala Aluganti Narasimhulu, Dinender K. Singla
Summary: Atherosclerosis is a chronic disease characterized by damage to the intima, inflammatory cell recruitment, and lipid accumulation followed by calcification and plaque rupture. Inflammation is believed to play a crucial role in the development and progression of the disease. This paper discusses the various types of inflammatory cells involved in atherosclerosis and their significance in the disease's development and progression. Understanding the role of these cells at different stages of the disease provides valuable insights for targeted therapy.
Editorial Material
Hematology
Wen Xuan Zhang, Paul Kubes
Summary: In this study, NOX2-deficient mice were used to investigate the underlying mechanisms of noninfectious inflammatory conditions in patients with CGD. The researchers identified a new population of AMs that exhibited significant inflammatory properties.
Article
Medicine, Research & Experimental
Zunyong Feng, Zhou Jing, Qiang Li, Liuxi Chu, YuXin Jiang, Xuanbo Zhang, Liang Yan, Yinhua Liu, Jing Jiang, Ping Xu, Qun Chen, Ming Wang, Hui Yang, Guoren Zhou, Xiaochun Jiang, Xiaoyuan Chen, Hongping Xia
Summary: Exosomes derived from AEC-IIs, called ADEs, can balance airway immunity and alleviate damage and fibrosis by regulating the immune and metabolic status of TRAMs. In a mouse fibrosis model, supplementation with ADEs reduced acute injury, prevented advanced fibrosis, alleviated ventilatory impairment, and decreased mortality.
Article
Pharmacology & Pharmacy
Sohaib Mahri, Eleonore Hardy, Tobias Wilms, Herlinde De Keersmaecker, Kevin Braeckmans, Stefaan De Smedt, Cynthia Bosquillon, Rita Vanbever
Summary: Conjugation with high molecular weight polyethylene glycol (PEG) significantly prolongs the lung residence time of recombinant human deoxyribonuclease I (rhDNase) by reducing transport across lung epithelial cells and uptake by macrophages, thus improving therapeutic efficacy after pulmonary delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Hector Garcia Romeu, Sarah Deville, Anna Salvati
Summary: This study introduces a flow cytometry-based method to easily detect and characterize the uptake and trafficking of nanoparticles within cells, providing spatial and temporal resolution data. The method enables high-throughput analysis of nanoparticle uptake and intracellular trafficking, allowing for the determination of how nanoparticle design influences their behavior within cells.
Article
Pharmacology & Pharmacy
Christophe Delehedde, Ivan Ciganek, Nathalie Rameix, Nabila Laroui, Cristine Goncalves, Luc Even, Patrick Midoux, Chantal Pichon
Summary: Targeting mRNA formulations to dendritic cells via mannose receptor can enhance the uptake of cationic lipopolyplexes and their routing to acidic compartments, but a high number of tri-mannose-lipid units can dramatically decrease transfection efficiency. Overcoming these side effects can be achieved by using modified mRNA or non-phosphorylation of NF-kappa B and PKR, as well as mTOR activation, to improve transfection efficiency.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Jitkasem Meewan, Sukrut Somani, Partha Laskar, Craig Irving, Margaret Mullin, Stuart Woods, Craig W. Roberts, Abdullah R. Alzahrani, Valerie A. Ferro, Suzanne McGill, Stefan Weidt, Richard Burchmore, Christine Dufes
Summary: The protein corona formed on the nanoparticle surface has a strong influence on cell interactions and uptake of nanomedicines. This study shows that the protein corona affects the uptake of PEGylated zein micelles by cancer cells, macrophages, and dendritic cells. PEGylation gives stealth effects to the zein micelles and makes them promising drug delivery systems for cancer therapy.
Article
Materials Science, Biomaterials
Zhenyu Liu, Jiake Lin, Benke Li, Yuemin Zhou, Chen Li, Yihao Cui, Fengchao Tian, Ruikang Tang, Xiaoyu Wang
Summary: This study presents a method for using manganese-mineralized tumor cells as whole-cell cancer vaccines, which improves the immunogenicity of tumor cells and overcomes immune suppression in solid tumors, leading to effective anti-tumor immune therapy.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Pharmacology & Pharmacy
Wayne Ngo, Sara Ahmed, Colin Blackadar, Bram Bussin, Qin Ji, Stefan M. Mladjenovic, Zahra Sepahi, Warren C. W. Chan
Summary: The accumulation of nanoparticles in diseased tissues is crucial for effective therapy and diagnosis. However, most nanoparticles end up being captured by liver macrophages after administration. This review describes the interactions between liver macrophages and nanoparticles, highlighting the advantages of liver macrophages in interacting with circulating nanoparticles.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Immunology
Ewelina Hoffman, Paulina Napieralska, Rhamiya Mahendran, Darragh Murnane, Victoria Hutter
Summary: This study aimed to distinguish between activated and non-activated alveolar macrophage using high content image analysis techniques, and to explore the generation process of 'foamy' macrophage phenotypes. The results showed distinct morphological and functional features of macrophages activated by LPS/IFN-gamma, as well as the proinflammatory characteristics of 'foamy' macrophages induced by certain compounds.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Siddharth Krishnan, Kelly Wemyss, Ian E. Prise, Flora A. McClure, Conor O'Boyle, Hayley M. Bridgeman, Tovah N. Shaw, John R. Grainger, Joanne E. Konkel
Summary: HSPCs were found to reside and differentiate in healthy gingiva, a key oral barrier. Gingival HSPCs exhibited similar characteristics to bone marrow HSPCs but responded differently to inflammatory insults, only responding to oral rather than systemic inflammation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Oncology
E. Le Reun, A. Casutt, A. Durham, H. Bouchaabe, E. Romano, A. Lovis, T. Krueger, C. Von Garniercg, E. M. Oezsahin, R. Kinj
Summary: This study compared three different radiotherapy devices for pulmonary stereotactic radiotherapy: CyberKnife, Helical Tomotherapy, and volumetric modulated arc therapy (VMAT). The results showed that CyberKnife treatments had comparable tumor volume, smaller planning treatment volume, and lower V5 values compared to other techniques. The incidence of toxicity was lower when V5 values were reduced. CyberKnife is particularly attractive for multiple courses of lung SBRT or lung reirradiation. Rating: 8/10
CANCER RADIOTHERAPIE
(2023)
Article
Respiratory System
Georgia Mitropoulou, Raphael Heinzer, Jean-Paul Janssens, Christophe von Garnier, Maura Prella
Summary: This study reported the real-life experience of adult patients using MI-E devices at home and identified factors associated with its use. Regular home use of MI-E was associated with increased symptom burden and overall satisfaction with the device, and it was not influenced by baseline cough peak flow, bulbar impairment, or non-invasive ventilation use.
Article
Environmental Sciences
Daria Korejwo, Savvina Chortarea, Chrysovalanto Louka, Marija Buljan, Barbara Rothen-Rutishauser, Peter Wick, Tina Buerki-Thurnherr
Summary: Graphene and its derivatives have a wide range of potential applications in various fields, but it is important to understand their potential adverse effects on human health. This study used gene expression profiling to investigate the transcriptional responses and toxicity pathways induced by graphene oxide (GO) and graphene nanoplatelets (GNP) in human macrophages. The results showed that GO and GNP caused differential expression of a large number of genes, with some overlap between the two types. Further analysis revealed that GNP mainly activated genes involved in inflammatory and apoptotic pathways, while GO had limited inflammatory responses and also affected genes related to antigen processing and presentation.
Article
Biochemistry & Molecular Biology
Giovanni Spiaggia, Patricia Taladriz-Blanco, Stefan Hengsberger, Dedy Septiadi, Christoph Geers, Aaron Lee, Barbara Rothen-Rutishauser, Alke Petri-Fink
Summary: Commercial static cell culture substrates have limited representation of in vivo biomechanical cues due to their inability to change physical properties over time. Gold nanoparticles have been used as transducers to overcome this limitation. In this study, gold nanorods were embedded in an elastomeric matrix to fabricate biocompatible light-responsive substrates. The analysis of the nanocomposite films showed a homogeneous heat distribution and increased stiffness when irradiated with NIR light. These films were biocompatible and cells remained viable, exhibiting a greater proliferation rate and stronger focal adhesion clustering.
Article
Oncology
E. Jamshidi, A. Asgary, S. Setareh, A. Casutt, M. Gonzalez, M. P. Bianchi, A. Lovis, M. De Palma, C. von Garnier, N. Mansouri
Summary: Using machine learning techniques, this study investigated the correlations between medical and personal characteristics of cancer patients and the risk of lung metastasis, leading to potential improvements in clinical management and outcomes. The study identified obesity, advanced age, and underlying lung disease as strong predictors for lung metastasis. The predictive model developed in this study can assist physicians in preventive risk factor control and treatment strategies.
Article
Immunology
Marc-Olivier Boldi, Justin Denis-Lessard, Rina Neziri, Rene Brouillet, Christophe von-Garnier, Valerie Chavez, Jesica Mazza-Stalder, Katia Jaton, Gilbert Greub, Onya Opota
Summary: Polymerase chain reactions (PCRs) have a significant impact on the diagnosis of tuberculosis (TB), with higher sensitivity and specificity than microscopy. Bronchial aspirate has a higher diagnostic yield than bronchoalveolar lavage (BAL) and sputum in respiratory specimens. Therefore, PCR should be performed on bronchial aspirates when available.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Allergy
Renaud Louis, Tim W. Harrison, Pascal Chanez, Francesco Menzella, George Philteos, Borja G. Cosio, Njira L. Lugogo, Gustavo de Luiz, Annie Burden, Timothy Adlington, Nanna Keeling, Justin Kwiatek, Esther Garcia Gil
Summary: This study demonstrates the ability of benralizumab to improve asthma control and allow for a reduction in background medications.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Medicine, General & Internal
Laurence Bamps, Jean-Philippe Armenti, Mirela Bojan, Bruno Grandbastien, Christophe von Garnier, Renaud Du Pasquier, Florian Desgranges, Matthaios Papadimitriou-Olivgeris, Lorenzo Alberio, Martin Preisig, Jurg Schwitter, Benoit Guery
Summary: This study collected health complaints from 474 patients one year after COVID-19 diagnosis and found that age, comorbidity index, and smoking habits were associated with hospital admission. Patients with a history of thromboembolism before SARS-CoV-2 infection had a higher risk of recurrence of thromboembolism at 1 year. Fatigue was the most common neurologic symptom, followed by feeling slowed down, headache, and smell disturbance. COVID-19 patients scored higher in anxiety, depression, fatigue, and sleep measures compared to the healthy population.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Food Science & Technology
Eva Susnik, Sandor Balog, Patricia Taladriz-Blanco, Alke Petri-Fink, Barbara Rothen-Rutishauser
Summary: This study investigated the interaction between nanoparticles (NPs) and intestinal epithelial cells, and found that cholera toxin subunit B (CTxB) did not significantly affect the membrane structure of differentiated Caco-2 cells or stimulate NP uptake, but it did reduce NP uptake in macrophages. The presence of CTxB was found to influence the uptake of NPs differently in phagocytic and differentiated intestine cells.
Article
Chemistry, Multidisciplinary
Giulia Mirabello, Lukas Steinmetz, Christoph Geers, Barbara Rothen-Ruthishauser, Mathias Bonmarin, Alke Petri-Fink, Marco Lattuada
Summary: This work demonstrates the application of lock-in thermography (LIT) in quantifying the concentration of gold nanoparticles embedded in polyvinyl alcohol (PVA) films. The study used films with different concentrations and sizes of gold nanoparticles and quantitatively analyzed the concentration by measuring the amplitude of the thermal signal emitted by the nanoparticles. The results showed that LIT is a reliable and non-destructive method to quantify nanoparticle concentrations and can detect concentration gradients in samples.
NANOSCALE ADVANCES
(2023)
Article
Engineering, Environmental
Jessica Caldwell, Alke Petri-Fink, Celine Loussert-Fonta, Gaelle Toullec, Niclas Heidelberg Lyndby, Beat Haenni, Patricia Taladriz-Blanco, Barbara Rothen-Rutishauser
Summary: Many researchers are studying the interaction between microplastics and marine fauna and working to monitor exposure pathways, concentrations, and their impacts. This study focuses on Cassiopea andromeda jellyfish, which live in (sub-)tropical coastal regions that are potentially contaminated by plastic waste. Fluorescent microplastics were detected using an optimized analytical protocol, and their interaction with the jellyfish was found to be influenced by the properties of the microplastics, such as density and hydrophobicity.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Radiology, Nuclear Medicine & Medical Imaging
Chiara Pozzessere, Christophe von Garnier, Catherine Beigelman-Aubry
Summary: Lung cancer screening programs using low-dose Computed Tomography (LDCT) are being implemented globally, but concerns over radiation exposure and potential risks may impact participation. This review aims to assess the radiation risk from LDCT in the screening program, providing estimates of cumulative dose and risk to improve awareness and attendance. International trials and factors influencing the radiation risk will be summarized, and future directions for personalized screening programs and dose reduction will be discussed.
Article
Chemistry, Multidisciplinary
Jessica Caldwell, Patricia Taladriz-Blanco, Laura Rodriguez-Lorenzo, Barbara Rothen-Rutishauser, Alke Petri-Fink
Summary: The presence of submicron- and nanoplastic particles has attracted increasing interest, but there are few analytical techniques capable of detecting these small plastic particles at low concentrations. This study investigates the potential of gold nanostar-based SERS substrates for the detection of submicron- and nanoplastic particles. The results demonstrate the promising potential of these substrates for nanoplastic detection, with variations in analytical performance depending on the properties of the plastics being studied.
ENVIRONMENTAL SCIENCE-NANO
(2023)